Difference between revisions of "Team:EPF Lausanne/Part Collection"
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Revision as of 16:27, 7 September 2015
E. coli.
dCas9-ω
dCa9s-ω is our main tool to build our project in E. coli. This catalytically dead Cas9 fused to the RNA polymerase ω subunit can act as an activator or as an inhibitor when provided the right guide RNA.
PAM rich URS J23117
This promoter is composed of the weak J23117 promoter, BBa_J23117, fused to a PAM rich upstream regulatory region (URS) that is needed for dCas9 to bind as dCas9 cannot bind without a PAM sequence.
sgRNA Z0
the gRNA Z0 inhibit PAM rich URS J23117 promoter when in complex with dCas9-ω.
sgRNA Z4
the gRNA Z4 activates PAM rich URS J23117 promoter when in complex with dCas9-ω.
