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Revision as of 09:55, 9 September 2015

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Labjournal Cellfree September

Fri, 04.09.

Cell-free Expression of HA-GFP-His6-His6, His10-GFP-Spy and GFP Bielefeld with KK and Promega

(Luisa)

  • 50 µl reactions were incubated at 37°C in the platereader (480/520 nm) for 4 hours in a 384 well plate. afterwards the foil covering the plate was removed a spectrum was obtained
  • comparison to GFP 1mg/ml dilutions
  • plate layout:
1 2 3 4 5
A HA-GFP-His6-His6 (KK) His10-GFP-Spy (KK) GFP Bielefeld (KK) no DNA (KK) air
B HA-GFP-His6-His6 (KK) His10-GFP-Spy (KK) GFP Bielefeld (KK) no DNA (KK) air
C HA-GFP-His6-His6 (KK) His10-GFP-Spy (KK) GFP Bielefeld (KK) no DNA (KK) air
D HA-GFP-His6-His6 (PP) His10-GFP-Spy (PP) GFP Bielefeld (PP) no DNA (PP) air
E HA-GFP-His6-His6 (PP) His10-GFP-Spy (PP) GFP Bielefeld (PP) no DNA (PP) air
F HA-GFP-His6-His6 (PP) His10-GFP-Spy (PP) GFP Bielefeld (PP) no DNA (PP) air
G GFP 1:10 GFP 1:20 GFP 1:40 GFP 1:80 air