Difference between revisions of "Team:Austin UTexas/Project/Spring Data"

 
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{{Austin_UTexas}}
 
{{Austin_UTexas}}
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<font color="017e70">--this is a first draft--</font>
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<font size="3">
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<br>
 
<h3>Spring 2015 Data Set</h3>
 
<h3>Spring 2015 Data Set</h3>
 
<hr>
 
<hr>
 
<hr>
 
<hr>
  
−
<font face="Courier New">Plasmid Construction</font>
+
<font face="Courier New">Plasmid Construction and Transformation</font>
 
<hr>
 
<hr>
 
<br>
 
<br>
−
<p>-----</p>
+
<p>Plasmids were constructed with standard BioBrick cloning procedures and enzymes. Composite plasmids were constructed using the following BioBrick combinations:</p>
−
[[Image:2015_Austin_UTexas_PLACEHOLDERFIGURE.png|right]]
+
<div align=center>
−
<p> ----- </p>
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BBa_K864100+BBa_K314100<p>
 +
BBa_E0020+BBa_K608007</p><p>
 +
BBa_EE030+BBa_K608006</p><p>
 +
BBa_E0020+BBa_K608003</p><p>
 +
BBa_E0030+BBa_K608007</p><p>
 +
BBa_K592101+BBa_K608006</p><p>
 +
BBa_K592100+BBa_K608004</p><p>
 +
BBa_K592101+BBa_K608002</p><p>
 +
BBa_K864100+BBa_K608006</p><p>
 +
BBa_E0030+BBa_K314100</p><p>
 +
BBa_E0020+BBa_K608002</p><p>
 +
BBa_K864100+BBa_K608002</p><p>
 +
BBa_E0030+BBa_K314100</p><p>
 +
BBa_K592101+BBa_K608007</p><p>
 +
<div align=left>
 +
<br>
 +
<font color="017e70">discuss what these BioBricks are<br>
 +
plasmids transformed by electroporation into pSB1c3</font>
 +
 
 
<br>
 
<br>
 
<br>
 
<br>
 
<font face="Courier New">Fluorescence Readings</font>
 
<font face="Courier New">Fluorescence Readings</font>
 
<hr>
 
<hr>
−
<p> -----</p>
+
[[Image:2015_Austin_UTexas_SpringsemesterBB-summary.png‎|500px|thumb|right|<b>Fig.2</b> description here]]
 +
<font color="017e70">relative fluorescence units<br>
 +
96 well plate<br>
 +
fluorescence data summary<br>
 +
summary figure(?)</font>
 
<br>
 
<br>
 
<br>
 
<br>
 
<font face="Courier New">Observations and Discussion</font>
 
<font face="Courier New">Observations and Discussion</font>
 
<hr>
 
<hr>
−
<p>-----</p>
+
<font color="017e70">insert mutations in SFYFP<br>
 +
EYFP, CYFP stable<br>
 +
what contributes to stability<br>
 +
what does this mean about what sequences are stable</br>
 +
<br>
 +
next steps<br>
 +
 
 
<br>
 
<br>
 
<br>
 
<br>
 
<br>
 
<br>

Latest revision as of 21:53, 10 September 2015

--this is a first draft--

Spring 2015 Data Set

Plasmid Construction and Transformation


Plasmids were constructed with standard BioBrick cloning procedures and enzymes. Composite plasmids were constructed using the following BioBrick combinations:

BBa_K864100+BBa_K314100

BBa_E0020+BBa_K608007

BBa_EE030+BBa_K608006

BBa_E0020+BBa_K608003

BBa_E0030+BBa_K608007

BBa_K592101+BBa_K608006

BBa_K592100+BBa_K608004

BBa_K592101+BBa_K608002

BBa_K864100+BBa_K608006

BBa_E0030+BBa_K314100

BBa_E0020+BBa_K608002

BBa_K864100+BBa_K608002

BBa_E0030+BBa_K314100

BBa_K592101+BBa_K608007


discuss what these BioBricks are
plasmids transformed by electroporation into pSB1c3



Fluorescence Readings

Fig.2 description here

relative fluorescence units
96 well plate
fluorescence data summary
summary figure(?)

Observations and Discussion

insert mutations in SFYFP
EYFP, CYFP stable
what contributes to stability
what does this mean about what sequences are stable</br>
next steps