Difference between revisions of "Team:NYU Shanghai/Protocols"
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Estimated time: 3 hours (plus 14-18 hour incubation) | Estimated time: 3 hours (plus 14-18 hour incubation) | ||
− | <br> | + | <br><br>Materials |
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<li id="transformText">2µl resuspended DNA (Resuspend well in 10ul dH20, pipette up and down several times, let sit for a few minutes)</li> | <li id="transformText">2µl resuspended DNA (Resuspend well in 10ul dH20, pipette up and down several times, let sit for a few minutes)</li> | ||
<li id="transformText">Competent cells (50ul per transformation)</li> | <li id="transformText">Competent cells (50ul per transformation)</li> | ||
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<li id="transformText">Spreader</li> | <li id="transformText">Spreader</li> | ||
<li id="transformText">37ºC incubator</li> | <li id="transformText">37ºC incubator</li> | ||
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<br><br>Procedure | <br><br>Procedure | ||
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Revision as of 19:13, 11 September 2015