Difference between revisions of "Team:NYU Shanghai/Protocols"

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  <h5 style="display:inline-block"><span class="noselect">Transformation</span></h5>   
 
  <h5 style="display:inline-block"><span class="noselect">Transformation</span></h5>   
 
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   Estimated time: 3 hours (plus 14-18 hour incubation)
 
   Estimated time: 3 hours (plus 14-18 hour incubation)
 
   <br><br>Materials
 
   <br><br>Materials
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     <li>Incubate the plates at 37ºC for 12-18 hours, making sure the agar side of the plate is up. If incubated for too long the antibiotics start to break down and un-transformed cells will begin to grow. This is especially true for ampicillin - because the resistance enzyme is excreted by the bacteria, and inactivates the antibiotic outside of the bacteria.</li>
 
     <li>Incubate the plates at 37ºC for 12-18 hours, making sure the agar side of the plate is up. If incubated for too long the antibiotics start to break down and un-transformed cells will begin to grow. This is especially true for ampicillin - because the resistance enzyme is excreted by the bacteria, and inactivates the antibiotic outside of the bacteria.</li>
 
     <li>You can pick a single colony, make a glycerol stock, grow up a cell culture and miniprep.</li>
 
     <li>You can pick a single colony, make a glycerol stock, grow up a cell culture and miniprep.</li>
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Revision as of 19:21, 11 September 2015

Protocols

Recipes

3A Assembly

Making Color