Difference between revisions of "Team:NYU Shanghai/Protocols"
m |
m |
||
| Line 199: | Line 199: | ||
<h5 style="display:inline-block"><span class="noselect">LB-Agar Plates</span></h5> | <h5 style="display:inline-block"><span class="noselect">LB-Agar Plates</span></h5> | ||
<br> | <br> | ||
| − | < | + | <div id="PlateText" style="display:none"> |
This is how you make an agar plate. | This is how you make an agar plate. | ||
| − | </ | + | </div> |
</div> | </div> | ||
| Line 207: | Line 207: | ||
<h5 style="display:inline-block"><span class="noselect">LB Broth</span></h5> | <h5 style="display:inline-block"><span class="noselect">LB Broth</span></h5> | ||
<br> | <br> | ||
| − | < | + | <div id="LBText" style="display:none"> |
This is how you make LB Broth. | This is how you make LB Broth. | ||
| − | </ | + | </div> |
</div> | </div> | ||
| Line 215: | Line 215: | ||
<h5 style="display:inline-block"><span class="noselect">SOC Media</span></h5> | <h5 style="display:inline-block"><span class="noselect">SOC Media</span></h5> | ||
<br> | <br> | ||
| − | < | + | <div id="SOCText" style="display:none"> |
This is how you make SOC media. | This is how you make SOC media. | ||
| − | </ | + | </div> |
</div> | </div> | ||
| Line 228: | Line 228: | ||
<br> | <br> | ||
<div id="transformText" style="display:none" font-family: "helveticaNL" font-size: "16px"> | <div id="transformText" style="display:none" font-family: "helveticaNL" font-size: "16px"> | ||
| − | Estimated time: 3 hours (plus | + | Estimated time: 3 hours (plus 14-18 hour incubation) |
<br><br>Materials | <br><br>Materials | ||
<li>2µl resuspended DNA | <li>2µl resuspended DNA | ||
| Line 255: | Line 255: | ||
<li>You can pick a single colony, make a glycerol stock, grow up a cell culture and miniprep.</li> | <li>You can pick a single colony, make a glycerol stock, grow up a cell culture and miniprep.</li> | ||
</ol> | </ol> | ||
| − | <br>Controls | + | <br><br>Controls |
<li>Competent cells on LB plate | <li>Competent cells on LB plate | ||
<li>Competent cells on LB + antibiotic plate | <li>Competent cells on LB + antibiotic plate | ||
| Line 265: | Line 265: | ||
<h5 style="display:inline-block"><span class="noselect">Miniprep</span></h5> | <h5 style="display:inline-block"><span class="noselect">Miniprep</span></h5> | ||
<br> | <br> | ||
| − | < | + | <div id="mpText" style="display:none"> |
This is how you miniprep. | This is how you miniprep. | ||
| − | </ | + | </div> |
</div> | </div> | ||
| Line 273: | Line 273: | ||
<h5 style="display:inline-block"><span class="noselect">Restriction Digest</span></h5> | <h5 style="display:inline-block"><span class="noselect">Restriction Digest</span></h5> | ||
<br> | <br> | ||
| − | < | + | <div id="rdText" style="display:none"> |
This is how you do restriction digest. | This is how you do restriction digest. | ||
| − | </ | + | </div> |
</div> | </div> | ||
| Line 281: | Line 281: | ||
<h5 style="display:inline-block"><span class="noselect">Ligation</span></h5> | <h5 style="display:inline-block"><span class="noselect">Ligation</span></h5> | ||
<br> | <br> | ||
| − | < | + | <div id="ligateText" style="display:none"> |
This is how you do ligation. | This is how you do ligation. | ||
| − | </ | + | </div> |
</div> | </div> | ||
| Line 289: | Line 289: | ||
<h5 style="display:inline-block"><span class="noselect">Gel Electrophoresis</span></h5> | <h5 style="display:inline-block"><span class="noselect">Gel Electrophoresis</span></h5> | ||
<br> | <br> | ||
| − | < | + | <div id="gelText" style="display:none"> |
This is how you do gel electrophoresis. | This is how you do gel electrophoresis. | ||
| − | </ | + | </div> |
</div> | </div> | ||
| Line 297: | Line 297: | ||
<h5 style="display:inline-block"><span class="noselect">Gel Extraction</span></h5> | <h5 style="display:inline-block"><span class="noselect">Gel Extraction</span></h5> | ||
<br> | <br> | ||
| − | < | + | <div id="gelexText" style="display:none"> |
This is how you do gel extraction. | This is how you do gel extraction. | ||
| − | </ | + | </div> |
</div> | </div> | ||
| Line 305: | Line 305: | ||
<h5 style="display:inline-block"><span class="noselect">PCR</span></h5> | <h5 style="display:inline-block"><span class="noselect">PCR</span></h5> | ||
<br> | <br> | ||
| − | < | + | <div id="PCRText" style="display:none"> |
This is the conditions we used for PCR. | This is the conditions we used for PCR. | ||
| − | </ | + | </div> |
</div> | </div> | ||
| Line 313: | Line 313: | ||
<h5 style="display:inline-block"><span class="noselect">PCR Cleanup</span></h5> | <h5 style="display:inline-block"><span class="noselect">PCR Cleanup</span></h5> | ||
<br> | <br> | ||
| − | < | + | <div id="PCRcleanText" style="display:none"> |
This is the conditions we used for PCR cleanup. | This is the conditions we used for PCR cleanup. | ||
| − | </ | + | </div> |
</div> | </div> | ||
| Line 325: | Line 325: | ||
<h5 style="display:inline-block"><span class="noselect">Luciferase</span></h5> | <h5 style="display:inline-block"><span class="noselect">Luciferase</span></h5> | ||
<br> | <br> | ||
| − | < | + | <div id="lucText" style="display:none"> |
This is the conditions we used to express luciferase. | This is the conditions we used to express luciferase. | ||
| − | </ | + | </div> |
</div> | </div> | ||
| Line 333: | Line 333: | ||
<h5 style="display:inline-block"><span class="noselect">Chromoproteins</span></h5> | <h5 style="display:inline-block"><span class="noselect">Chromoproteins</span></h5> | ||
<br> | <br> | ||
| − | < | + | <div id="chromoText" style="display:none"> |
This is the conditions we used to express chromoproteins. | This is the conditions we used to express chromoproteins. | ||
| − | </ | + | </div> |
<br> | <br> | ||
<br> | <br> | ||
Revision as of 19:50, 11 September 2015
-
-
Project
-
Team
-
Outreach
-
Notebook