Difference between revisions of "Team:UMaryland/Composite Part"
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| − | Combines <a href = "http://parts.igem.org/Part:BBa_K206000">BBa_K206000</a> (pBAD promoter) with <a href = "http://parts.igem.org/Part:BBa_K1033120">BBa_K1033120</a> (B0034-ExpTag-Linker-Miraculin-Linker-6xHis). Codes for a His-tagged miraculin with export tag to periplasm. Induced with arabinose. Miraculin is a protein that interacts with our taste receptors, causing us to taste sour foods as sweet. This part was assembled using 3A Assembly as a training exercise for new UMaryland iGEM members. | + | <p style = "font-size:18px">Combines <a href = "http://parts.igem.org/Part:BBa_K206000">BBa_K206000</a> (pBAD promoter) with <a href = "http://parts.igem.org/Part:BBa_K1033120">BBa_K1033120</a> (B0034-ExpTag-Linker-Miraculin-Linker-6xHis). Codes for a His-tagged miraculin with export tag to periplasm. Induced with arabinose. Miraculin is a protein that interacts with our taste receptors, causing us to taste sour foods as sweet. This part was assembled using 3A Assembly as a training exercise for new UMaryland iGEM members.</p> |
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| − | Complete with a constitutive promoter, this fluorescent protein is expressed continuously in the cytoplasm. Due to the presence of a LVA degradation tag, this protein is subject to rapid targeting via proteases, with a half life of about 1 hour. | + | <p style = "font-size:18px">Complete with a constitutive promoter, this fluorescent protein is expressed continuously in the cytoplasm. Due to the presence of a LVA degradation tag, this protein is subject to rapid targeting via proteases, with a half life of about 1 hour.</p> |
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| − | This part combines two of our previous parts: Hok-Sok TA cassette and Constitutive Unstable RFP. This testing device was used to measure the ability of the Hok-Sok maintenance system to force cells to retain plasmids. The presence of Hok-Sok adjacent to the constitutive promoter for unstable RFP was found to interfere with RFP production in a minor way. | + | <p style = "font-size:18px">This part combines two of our previous parts: Hok-Sok TA cassette and Constitutive Unstable RFP. This testing device was used to measure the ability of the Hok-Sok maintenance system to force cells to retain plasmids. The presence of Hok-Sok adjacent to the constitutive promoter for unstable RFP was found to interfere with RFP production in a minor way.</p> |
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Revision as of 22:35, 11 September 2015
