Difference between revisions of "Team:Freiburg/Glossary"

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<div>
 
<div>
 
     <h4>Glossary</4>
 
     <h4>Glossary</4>
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</div>
 
         <div class="table sectionedit2"><table class="inline">  
 
         <div class="table sectionedit2"><table class="inline">  
 
             <tr class="row0">
 
             <tr class="row0">
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             <td class="col1"> classical cloning </td><td class="col0"> insertion of a DNA fragment for example into a plasmid backbone via restriction enzymes; each fragment has to be inserted separately. For differences to Gibson cloning see <a href="https://2015.igem.org/Team:Freiburg/Project/Classic_vs_Gibson"> here </a> </td></tr>
 
             <td class="col1"> classical cloning </td><td class="col0"> insertion of a DNA fragment for example into a plasmid backbone via restriction enzymes; each fragment has to be inserted separately. For differences to Gibson cloning see <a href="https://2015.igem.org/Team:Freiburg/Project/Classic_vs_Gibson"> here </a> </td></tr>
 
           <tr class="row7">
 
           <tr class="row7">
−
             <td class="col1"> </td><td class="col0">  </td></tr>
+
             <td class="col1"> Cy3 </td><td class="col0"> Cyanine 3, fluorescent dye used particularly in biomedical imaging </td></tr>
 
           <tr class="row8">
 
           <tr class="row8">
−
             <td class="col1"> </td><td class="col0"> </td></tr>
+
             <td class="col1"> DE3 </td><td class="col0"> nomenclature of <i>E.coli</i> strains, meaning it contains a T7 RNA-polymerase for general protein expression </td></tr>
 
           <tr class="row9">
 
           <tr class="row9">
 +
            <td class="col1"> DMSO </td><td class="col0"> Dimethylsulfoxid, added to PCR reactions </td></tr>
 +
          <tr class="row10">
 +
            <td class="col1"> dNTP </td><td class="col0"> deoxynucleotidetriphosphate, component of DNA and therefore added to PCR reaction mixes </td></tr>
 +
          <tr class="row11">
 +
            <td class="col1"> Dot Blot </td><td class="col0"> simplification of Northern, Southern or Western Blot in order to save time; proteins are not separated electrophoretically but simply spotted on a membrane and then detected via nucleotide probes (Northern and Southern Blot) or antibodies (Western Blot) </td></tr>
 +
          <tr class="row12">
 +
            <td class="col1"> ELISA </td><td class="col0"> enzyme-linked immunosorbent assay, antigens are immobilized in a 96-well plate, antibody-antigen-interaction is detected; find more information <a href="https://2015.igem.org/Team:Freiburg/Project/Diagnostics"> here </a> </td></tr>
 +
          <tr class="row13">
 +
            <td class="col1"> fluorophore </td><td class="col0"> fluorescent chemical compound that can emit light upon light excitation; typically contains several combined aromatic groups or plane or cyclic molecules with several π bonds </td></tr>
 +
          <tr class="row14">
 +
            <td class="col1"> GFP </td><td class="col0"> green fluorescent protein </td></tr>
 +
          <tr class="row15">
 +
            <td class="col1"> Gibson Assembly </td><td class="col0"> combination of DNA fragments in an isothermal reaction without the need of restriction enzymes; more information and a comparison to classical cloning can be found <a href="https://2015.igem.org/Team:Freiburg/Project/Classic_vs_Gibson"> here </a> </td></tr>
 +
          <tr class="row16">
 +
            <td class="col1"> glycoprotein </td><td class="col0"> surface protein with oligosaccharide chains covalently bound to the polypeptide; also present on virus surfaces and often provoking an immunogenic response in humans </td></tr>
 +
          <tr class="row17">
 +
            <td class="col1"> HA-tag </td><td class="col0"> tag derived from the human influenza hemagglutinin and extensively used as a general epitope tag in expression vectors. </td></tr>
 +
          <tr class="row18">
 +
            <td class="col1"> Her2 protein </td><td class="col0"> biomarker for breast cancer </td></tr>
 +
          <tr class="row19">
 +
            <td class="col1"> His6 </td><td class="col0"> tag consisting of six histidine residues binding to Ni-NTA and often used for protein purification </td></tr>
 +
          <tr class="row20">
 +
            <td class="col1"> HIV </td><td class="col0"> Human Immunodeficiency Virus, causes AIDS </td></tr>
 +
          <tr class="row21">
 +
            <td class="col1"> HSV-1 </td><td class="col0"> Herpes Simplex Virus type 1, also known as Human Herpes Virus type 3 </td></tr>
 +
          <tr class="row22">
 +
            <td class="col1"> IPTG </td><td class="col0"> Isopropylthiogalactopyranosid, allolactose analogue used to induce the lacI system in cells; often used to induce protein overexpression in bacteria </td></tr>
 +
          <tr class="row23">
 +
            <td class="col1">  </td><td class="col0">  </td></tr>
 +
          <tr class="row24">
 
             <td class="col1">  </td><td class="col0">  </td></tr>
 
             <td class="col1">  </td><td class="col0">  </td></tr>
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+
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 +
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 +
          <tr class="row26">
 +
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 +
          <tr class="row27">
 +
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 +
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 +
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 +
          <tr class="row29">
 
             <td class="col1">  </td><td class="col0">  </td></tr>
 
             <td class="col1">  </td><td class="col0">  </td></tr>
  

Revision as of 14:33, 12 September 2015

""

Glossary

term definition
5’UTR an untranslated region at the 5’ end of the RNA sequence that enhances translation
affibody antibody mimetic, small protein engineered to bind to a large number of target proteins or peptides with high affinity, imitating monoclonal antibodies
AIDS Acquired Immune Deficiency Syndrome, foll-blown diseases after infection with HIV
APTES 3-aminopropyltriethoxysilane, a silane we used to link PDITC to the hydroxyl groups on the plasma activated glass slide of our protein array
CFP cyan fluorescent protein
classical cloning insertion of a DNA fragment for example into a plasmid backbone via restriction enzymes; each fragment has to be inserted separately. For differences to Gibson cloning see here
Cy3 Cyanine 3, fluorescent dye used particularly in biomedical imaging
DE3 nomenclature of E.coli strains, meaning it contains a T7 RNA-polymerase for general protein expression
DMSO Dimethylsulfoxid, added to PCR reactions
dNTP deoxynucleotidetriphosphate, component of DNA and therefore added to PCR reaction mixes
Dot Blot simplification of Northern, Southern or Western Blot in order to save time; proteins are not separated electrophoretically but simply spotted on a membrane and then detected via nucleotide probes (Northern and Southern Blot) or antibodies (Western Blot)
ELISA enzyme-linked immunosorbent assay, antigens are immobilized in a 96-well plate, antibody-antigen-interaction is detected; find more information here
fluorophore fluorescent chemical compound that can emit light upon light excitation; typically contains several combined aromatic groups or plane or cyclic molecules with several π bonds
GFP green fluorescent protein
Gibson Assembly combination of DNA fragments in an isothermal reaction without the need of restriction enzymes; more information and a comparison to classical cloning can be found here
glycoprotein surface protein with oligosaccharide chains covalently bound to the polypeptide; also present on virus surfaces and often provoking an immunogenic response in humans
HA-tag tag derived from the human influenza hemagglutinin and extensively used as a general epitope tag in expression vectors.
Her2 protein biomarker for breast cancer
His6 tag consisting of six histidine residues binding to Ni-NTA and often used for protein purification
HIV Human Immunodeficiency Virus, causes AIDS
HSV-1 Herpes Simplex Virus type 1, also known as Human Herpes Virus type 3
IPTG Isopropylthiogalactopyranosid, allolactose analogue used to induce the lacI system in cells; often used to induce protein overexpression in bacteria