Difference between revisions of "Team:Freiburg/Project"
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<h1 class="sectionedit1">Project overview: The DiaCHIP</h1> | <h1 class="sectionedit1">Project overview: The DiaCHIP</h1> | ||
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The DiaCHIP is an innovative tool to screen for a broad range of antibodies present in serum. <br> | The DiaCHIP is an innovative tool to screen for a broad range of antibodies present in serum. <br> | ||
Antibodies can be an indicator for an immune response towards an infection or a successful vaccination. <br> Antibodies also play a role in the diagnosis of autoimmune diseases. <br> | Antibodies can be an indicator for an immune response towards an infection or a successful vaccination. <br> Antibodies also play a role in the diagnosis of autoimmune diseases. <br> | ||
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The key feature of the DiaCHIP concept is the combination of on-demand protein synthesis and a novel method for label-free detection packed into one device. The idea is to overcome challenges commonly found in protein array production and preservation. In addition, results can be obtained in a time- and cost-efficient manner; with a device simple enough to be rebuilt by future iGEM teams. | The key feature of the DiaCHIP concept is the combination of on-demand protein synthesis and a novel method for label-free detection packed into one device. The idea is to overcome challenges commonly found in protein array production and preservation. In addition, results can be obtained in a time- and cost-efficient manner; with a device simple enough to be rebuilt by future iGEM teams. | ||
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<b>Step 1: Preparing the DiaCHIP by protein synthesis</b> | <b>Step 1: Preparing the DiaCHIP by protein synthesis</b> | ||
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Prior to screening for antibody-antigen interactions, the antigens have to be synthesized and immobilized in a microarray set-up. The DiaCHIP facilitates this process by a copying mechanism, which converts a DNA template into a protein microarray by cell-free protein expression. This expression system based on a bacterial lysate makes the need for genetically engineered organisms to produce each single antigen redundant. | Prior to screening for antibody-antigen interactions, the antigens have to be synthesized and immobilized in a microarray set-up. The DiaCHIP facilitates this process by a copying mechanism, which converts a DNA template into a protein microarray by cell-free protein expression. This expression system based on a bacterial lysate makes the need for genetically engineered organisms to produce each single antigen redundant. | ||
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<b>Step 2: Measuring serum samples by iRIf</b> | <b>Step 2: Measuring serum samples by iRIf</b> | ||
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After preparation of the DiaCHIP, a patient’s serum sample can be flushed over the protein array. The binding of antibodies to the protein surface causes a minimal change in the thickness of the slide right at the corresponding antigen spot. This change can be measured without the need for a further label with an emerging method called iRIf (imaging reflectometric interference). Based on the interference of light beams reflected on different thin layers, binding events can be recorded in real-time. | After preparation of the DiaCHIP, a patient’s serum sample can be flushed over the protein array. The binding of antibodies to the protein surface causes a minimal change in the thickness of the slide right at the corresponding antigen spot. This change can be measured without the need for a further label with an emerging method called iRIf (imaging reflectometric interference). Based on the interference of light beams reflected on different thin layers, binding events can be recorded in real-time. | ||
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Revision as of 20:32, 13 September 2015
Project overview: The DiaCHIP
The DiaCHIP is an innovative tool to screen for a broad range of antibodies present in serum.
Antibodies can be an indicator for an immune response towards an infection or a successful vaccination.
Antibodies also play a role in the diagnosis of autoimmune diseases.
Especially the ability to differentiate between life threatening diseases and a mild infection within a short time bears the potential to save lives.
Spotting diseases by detecting correspondent antibodies in a patient's serum is an established method in modern diagnostics. The DiaCHIP makes it possible to screen for multiple specific antibodies simply using a drop of blood.
The key feature of the DiaCHIP concept is the combination of on-demand protein synthesis and a novel method for label-free detection packed into one device. The idea is to overcome challenges commonly found in protein array production and preservation. In addition, results can be obtained in a time- and cost-efficient manner; with a device simple enough to be rebuilt by future iGEM teams.
