Difference between revisions of "Team:NYU Shanghai/Protocols"
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</div> | </div> | ||
− | <div> | + | <div id="recipes"> |
<h4>Recipes</h4> | <h4>Recipes</h4> | ||
− | |||
<div id="Plate" class="collapsed" onclick="expandPlate()"> | <div id="Plate" class="collapsed" onclick="expandPlate()"> | ||
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<div id="SOCText" style="display:none"> | <div id="SOCText" style="display:none"> | ||
This is how you make SOC media. | This is how you make SOC media. | ||
+ | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
− | <div> | + | <div id="3aAssembly"> |
<h4>3A Assembly</h4> | <h4>3A Assembly</h4> | ||
− | |||
<div id="transform" class="collapsed" onclick="expandtransform()"> | <div id="transform" class="collapsed" onclick="expandtransform()"> | ||
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Estimated time: 3 hours (plus 14-18 hour incubation) | Estimated time: 3 hours (plus 14-18 hour incubation) | ||
<br><br>Materials | <br><br>Materials | ||
− | <li>2µl resuspended DNA | + | <li>2µl resuspended DNA</li> |
− | <li>Competent cells (50ul per transformation) | + | <li>Competent cells (50ul per transformation)</li> |
− | <li>Ice | + | <li>Ice</li> |
− | <li>2ml tube | + | <li>2ml tube</li> |
− | <li>42ºC water bath | + | <li>42ºC water bath</li> |
− | <li>SOC or LB media | + | <li>SOC or LB media</li> |
− | <li>1 LB plate and 1 LB+antibiotic per transformation | + | <li>1 LB plate and 1 LB+antibiotic per transformation</li> |
− | <li>Spreader | + | <li>Spreader</li> |
− | <li>37ºC incubator | + | <li>37ºC incubator</li> |
− | <li>Shaker | + | <li>Shaker</li> |
<br><br>Procedure | <br><br>Procedure | ||
<ol> | <ol> | ||
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</ol> | </ol> | ||
<br>Controls | <br>Controls | ||
− | <li>Competent cells on LB plate | + | <li>Competent cells on LB plate</li> |
− | <li>Competent cells on LB + antibiotic plate | + | <li>Competent cells on LB + antibiotic plate</li> |
− | <li>Competent cells + plasmid on LB plate | + | <li>Competent cells + plasmid on LB plate</li> |
</div> | </div> | ||
</div> | </div> | ||
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<div id="PCRcleanText" style="display:none"> | <div id="PCRcleanText" style="display:none"> | ||
This is the conditions we used for PCR cleanup. | This is the conditions we used for PCR cleanup. | ||
+ | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
− | <div> | + | <div id="makingColor"> |
<h4>Making Color</h4> | <h4>Making Color</h4> | ||
− | |||
<div id="luc" class="collapsed" onclick="expandluc()"> | <div id="luc" class="collapsed" onclick="expandluc()"> | ||
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<br> | <br> | ||
<br> | <br> | ||
+ | </div> | ||
</div> | </div> | ||
Revision as of 05:32, 14 September 2015