Difference between revisions of "Team:NYU Shanghai/Protocols"
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</ul><br><br> | </ul><br><br> | ||
<p><em>Note:</em> Some formulations of SOB use 10 mM MgCl2 and 10 mM MgSO4 instead of 20 mM MgSO4. | <p><em>Note:</em> Some formulations of SOB use 10 mM MgCl2 and 10 mM MgSO4 instead of 20 mM MgSO4. | ||
− | SOB medium is also available dry premixed from Difco, 0443-17.<br>Adjust to pH 7.5 prior to use. This requires approximately 25 ml of 1M NaOH per liter.<br><b>15/10 medium</b><br>Growth of competent TOP10 cells in Example 2 of the Bloom05 patent is performed in 15/10 broth, which is similar to SOB:</p> | + | SOB medium is also available dry premixed from Difco, 0443-17.<br>Adjust to pH 7.5 prior to use. This requires approximately 25 ml of 1M NaOH per liter.<br><br><b>15/10 medium</b><br><br>Growth of competent TOP10 cells in Example 2 of the Bloom05 patent is performed in 15/10 broth, which is similar to SOB:</p> |
<ul> | <ul> | ||
<li>1.5% yeast extract</li> | <li>1.5% yeast extract</li> |
Revision as of 05:49, 14 September 2015