Difference between revisions of "Team:NYU Shanghai/Protocols"
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<h3>Protocols</h3> | <h3>Protocols</h3> | ||
+ | <h6>We built our constructs from digests and ligations of pre-made biobrick parts. Our general conclusion is that 3A assembly is generally inefficient, and an insufficient method for adding small parts (such as a terminator) to a larger construction within pSB1C3. We learned that ratios were extremely important in the process of 3A Assembly, and we made a summary sheet of the equations we used in pre-digest and pre-ligation that accounts for digest dilution and amount needed to ensure results are seen on a gel, not just ligation ratios. </h6> | ||
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<h4>3A Assembly</h4> | <h4>3A Assembly</h4> | ||
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<div id="transform" class="collapsed" onclick="expandtransform()"> | <div id="transform" class="collapsed" onclick="expandtransform()"> | ||
<h5 style="display:inline-block"><span class="noselect">Transformation</span></h5> | <h5 style="display:inline-block"><span class="noselect">Transformation</span></h5> |
Revision as of 12:34, 15 September 2015