Difference between revisions of "Team:Goettingen/Notebook"

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<a href="" onClick=" $('#menu23').slideToggle(300, function callback() {  }); return false;"><h2>13.04.15</h2></a>
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<div id="menu23">
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<p>
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<p>
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    <strong>1.) Website:</strong>
 +
</p>
 +
<p>
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    Individual skills need to be worked out to find out who can do which part. Joschy is currently learning how to use HTML. He has made a navigation bar. It
 +
    may be helpful to have some external input.
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</p>
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<p>
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    The colour scheme will be our iGEM colours (blues)
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</p>
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<p>
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    The content must be updateable by every group, can be taken from the booklet to start with
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</p>
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<p>
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    Every team member needs to write a max. 150 word paragraph about themselves and submit a photograph to the dropbox.
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</p>
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<p>
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    <strong>2.) Labwork:</strong>
 +
</p>
 +
<p>
 +
    Dockerins: DNA has been extracted and we may get 3-4 Dockerins. Dockerins need to be finished in order to have the enzyme groups continue.
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</p>
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<p>
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    Scaffoldin: Transformation into E.coli worked and the plasmids can now be extracted and verified. First expression tests can follow. Alaa and Sindhu will
 +
    continue with the Scaffoldin from tomorrow.
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</p>
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<p>
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    E2: Primers will be designed on Thursday
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</p>
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<p>
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    E1: nothing to report due to a Practical, no work can be done atm.
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</p>
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<p>
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    Clone Manager: Julian will try to see if we can get access to it somehow.
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</p>
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<p>
 +
    <strong>3.) Sponsors:</strong>
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</p>
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<p>
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    GZMB is meeting soon and will decide on funding. We have so far got 4 Sponsors.
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</p>
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<p>
 +
    <strong>4.) Flights:</strong>
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</p>
 +
<p>
 +
    Alaa has looked up flights if we book now:
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</p>
 +
<p>
 +
    700-870€, Hotel: 45-60€ p.P /pN. We need to book fast as most hotels and hostels will be booked by fellow igemers.
 +
</p>
 +
<p>
 +
    <strong>5.) Costs:</strong>
 +
</p>
 +
<p>
 +
    Anticipated Labcosts: 18000€
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</p>
 +
<p>
 +
    Flights: 800€ pP
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</p>
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<p>
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    Hotel: 600€ pP
 +
</p>
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<p>
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    We need a minimum of 6000€ from the GZMB if we want to continue with the project.
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</p>
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</div>
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 +
<a href="" onClick=" $('#menu24').slideToggle(300, function callback() {  }); return false;"><h2>21.05.15 </h2></a>
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<div id="menu24">
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<p>
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<p>
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    About the supervisors needs to be written for our Webpage
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</p>
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<p>
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    <strong>Labwork:</strong>
 +
</p>
 +
<p>
 +
    Dockerins: Attempt to put them in pBAD has failed so far. Retransformation control worked however. ACEL needs to be reinserted into pJET, BCEL needs to be
 +
    restarted from PCR, CTHE ligation needs to be redone. Change elution steps to gain higher concentrations (30µl instead of 50)
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</p>
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</div>
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<a href="" onClick=" $('#menu13').slideToggle(300, function callback() {  }); return false;"><h2> 19.0615</h2></a>
 
<a href="" onClick=" $('#menu13').slideToggle(300, function callback() {  }); return false;"><h2> 19.0615</h2></a>
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</p>
 
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<a href="" onClick=" $('#menu17').slideToggle(300, function callback() {  }); return false;"><h2>24.07.15</h2></a>
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<div id="menu17">
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<p>
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    Present: Debbie, Avi, Julian, DEnnis
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</p>
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<p>
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    Excused: Verena, Steffi, Nida, Sindhu
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</p>
 +
<p>
 +
    <strong>1.) Labwork:</strong>
 +
</p>
 +
<p>
 +
    Enzyme 3: Transformations didn't work, so the project will be restarted from PCR stage and a new ligation.
 +
</p>
 +
<p>
 +
    The sequencing is also not working, possibly due to wrong primers or wrong primer concentrations.
 +
</p>
 +
<p>
 +
    Dockerin/Scaffoldin: both scaffoldins are at the expression stage
 +
</p>
 +
<p>
 +
    Dockerins in gBlocks have arrived.
 +
</p>
 +
<p>
 +
    Old Dockerins may not be restrictable due to missing bands around the restriction sites.
 +
</p>
 +
<p>
 +
    New Plasmid isolation is on the way and work will continue on the dockerins as before.
 +
</p>
 +
<p>
 +
    <strong>2.) 3D Model:</strong>
 +
</p>
 +
<p>
 +
    Debbie has checked the sequences and has the Amino Acid sequences for all enzymes, but cannot find the scaffoldin sequences. When used they result in short
 +
    fragments. Dockerins do not work either due to too many stopcodons.
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</p>
 +
<p>
 +
    Double check the files and give her new files.
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</p>
 +
<p>
 +
    <strong>3.) Human Practices:</strong>
 +
</p>
 +
<p>
 +
    Julian will contact people at the Xlab.
 +
</p>
 +
<p>
 +
    A stop motion movie is being made that simply explains our project
 +
</p>
 +
<p>
 +
    A talk needs to be organised around our project and synthetic biology -&gt; Beer and Pretzels? Julian will contact some people and organise it.
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</p>
 +
<p>
 +
    Once returned from iGEM, the presenters will also present their project to the faculty at a faculty talk on the 27.10.2015
 +
</p>
 +
<p>
 +
    <strong>4.) Meetup:</strong>
 +
</p>
 +
<p>
 +
    The group that will travel to Marburg for the German Meetup will prepare short presentation
 +
</p>
 +
<p>
 +
    <strong>5.) Poster:</strong>
 +
</p>
 +
<p>
 +
    Dennis is happy to help with the organisation of the poster
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</p>
  
 
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Revision as of 14:13, 15 September 2015



Notebook

15.12.14

08.01.15

13.01.15

15.01.15

19.01.15

02.02.15

16.02.15

18.02.15

23.02.15

16.03.15

23.3.15

07.04.15

13.04.15

21.05.15

19.0615

25.06.15

24.07.15