Difference between revisions of "Team:METU Turkey/Experiments"
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remove residual Wash Solution. This step is essential to avoid residual | remove residual Wash Solution. This step is essential to avoid residual | ||
ethanol in plasmid preps.Transfer the GneJET spin column into a fresh | ethanol in plasmid preps.Transfer the GneJET spin column into a fresh | ||
− | 1.5 ml microcentrifuge tube. Add 50μL of the Elution Buffer to the | + | 1.5 ml microcentrifuge tube. Add 50μL of the Elution Buffer to the |
− | of GeneJET spin column membrane to elute the plasmid DNA. Take care | + | center of GeneJET spin column membrane to elute the plasmid DNA. |
− | not to contact the membrane with the pipette tip. Incubate for 2 min at | + | Take care not to contact the membrane with the pipette tip. Incubate |
− | room temperature and centrifuge for 2 min. | + | for 2 min at room temperature and centrifuge for 2 min. |
Discard the column and store the purified plasmid DNA at -20 ˚C. | Discard the column and store the purified plasmid DNA at -20 ˚C. | ||
Revision as of 15:16, 15 September 2015
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