Difference between revisions of "Team:NYU Shanghai/Protocols"
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<li>After autoclave, move to the hood. Let the LB agar cool until it’s less than 60ºC or until you can touch the flask. While waiting, you can start labeling the plates.</li> | <li>After autoclave, move to the hood. Let the LB agar cool until it’s less than 60ºC or until you can touch the flask. While waiting, you can start labeling the plates.</li> | ||
<li>Insert any antibiotics/sugars needed. If you are not sure about the ratio, you can follow this simple example: | <li>Insert any antibiotics/sugars needed. If you are not sure about the ratio, you can follow this simple example: | ||
− | <p style="text-align: center;">50mL LB + 500µL Arabinose* + 50µL Ampicillin**<span style="font-size: | + | <p style="text-align: center;"><b>50mL LB + 500µL Arabinose* + 50µL Ampicillin**</b><span style="font-size: 10;"><br><i> |
*2mg/mL of Arabinose Stock Solution<br> | *2mg/mL of Arabinose Stock Solution<br> | ||
− | **100mg/mL of Ampicillin Stock Solution</span></p> | + | **100mg/mL of Ampicillin Stock Solution</i></span></p> |
</li> | </li> | ||
<li>Swirl the antibiotics/sugars that you just added for a minute.</li> | <li>Swirl the antibiotics/sugars that you just added for a minute.</li> |
Revision as of 03:45, 16 September 2015