Difference between revisions of "Team:Freiburg/Project/pRIG15 6"
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| − | The Rubella Virus causes the Rubella disease or the so-called „German measles“. It is a typical children’s disease and can get dangerous when first infection occurs after childhood. In this <a class="urlextern" href="http://parts.igem.org/Part:BBa_K1621000" rel="nofollow" target="_Blank" title="http://parts.igem.org/Part:BBa_K1621000">biobrick</a> we include the sequence for the Rubella Glycoprotein E1 which is an envelope associated protein <sup><a class="fn_top" href="#fn__1" id="fnt__1" name="fnt__1">1)</a></sup>. | + | |
| − | To insert the sequence for rubella glycoprotein E1 into pSB1C3 we designed Gibson primers with compatible overhangs that also included the start codon ATG. The insert was amplified via | + | The Rubella Virus causes the Rubella disease or the so-called <a class="wikilink1" href="https://2015.igem.org/Team:Freiburg/Project/Diseases#Measles" title=„German measles“>German measles</a>. It is a typical children’s disease and can get dangerous when first infection occurs after childhood. In this <a class="urlextern" href="http://parts.igem.org/Part:BBa_K1621000" rel="nofollow" target="_Blank" title="http://parts.igem.org/Part:BBa_K1621000">biobrick</a> we include the sequence for the Rubella Glycoprotein E1 which is an envelope associated protein <sup><a class="fn_top" href="#fn__1" id="fnt__1" name="fnt__1">1)</a></sup>. |
| + | To insert the sequence for rubella glycoprotein E1 into <a class="urlextern" href="http://parts.igem.org/Part:pSB1C3" target="_blank" title="pSB1C3">pSB1C3</a> we designed Gibson primers with compatible overhangs that also included the start codon ATG. The insert was amplified via <a class="wikilink1" href="https://2015.igem.org/Team:Freiburg/Labjournals/Cloning/August#PCR6-18" title="PCR6-18">PCR</a> and then assembled with the digested <a class="urlextern" href="http://parts.igem.org/Part:pSB1C3" target="_blank" title="pSB1C3">pSB1C3</a> backbone using <a class="wikilink1" href="https://2015.igem.org/Team:Freiburg/Project/Classic_vs_Gibson" ttle="gibson">Gibson</a> assembly. | ||
</p> | </p> | ||
| − | < | + | |
| − | <div class=" | + | <div class="image_box right"> |
| − | < | + | <div class="thumb2 trien" style="width:310px"> |
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| + | <a href="https://static.igem.org/mediawiki/2015/3/34/Freiburg_labjournal-cloning-prig15_6.jpg" class="lightbox_trigger"> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/3/34/Freiburg_labjournal-cloning-prig15_6.jpg" width="300px"> | ||
| + | <div class="thumbcaption"> | ||
| + | </a> | ||
| + | <p>Figure 1: pRIG15_6. BBa_K1621000 inserted into the submission backbone pSB1C3.</p> | ||
| + | </div> | ||
| + | </div> | ||
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| − | </ | + | </div> |
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<p> | <p> | ||
To prove correct insertion of our fragment we did a test digest (Link Labjournal) and sent the whole plasmid for sequencing (GATC). | To prove correct insertion of our fragment we did a test digest (Link Labjournal) and sent the whole plasmid for sequencing (GATC). | ||
| + | </br> | ||
| + | </br> | ||
| + | Link to GeneBank file: <a class="media" href="https://static.igem.org/mediawiki/2015/2/26/2015_Freiburg_BBa_K1621000.gb" title="2015_Freiburg_BBa_K1621000" src="https://static.igem.org/mediawiki/2015/2/26/2015_Freiburg_BBa_K1621000.gb">BBa_K1621000.gb</a>. | ||
| + | </br> | ||
| + | Link to Registry: <a href="http://parts.igem.org/Part:BBa_K1621000" target="_blank">BBa_K1621000</a> | ||
</p> | </p> | ||
</div> | </div> | ||
<div class="footnotes"> | <div class="footnotes"> | ||
<div class="fn"><sup><a class="fn_bot" href="#fnt__1" id="fn__1" name="fn__1">1)</a></sup> | <div class="fn"><sup><a class="fn_bot" href="#fnt__1" id="fn__1" name="fn__1">1)</a></sup> | ||
| − | Starkey, W. G., Newcombe, J., Corbett, K. M., Liu, K. M., Sanders, P.G., Best, J.M, (1995) Use of Rubella Virus E1 Fusion Proteins for Detection of Rubella Virus Antibodies. Journal of Clinical Microbiology | + | <a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC227930/" target="_blank">Starkey, W. G., Newcombe, J., Corbett, K. M., Liu, K. M., Sanders, P.G., Best, J.M, (1995) Use of Rubella Virus E1 Fusion Proteins for Detection of Rubella Virus Antibodies. Journal of Clinical Microbiology.</a> |
| + | </div> | ||
</div> | </div> | ||
Latest revision as of 22:35, 16 September 2015
pRIG15_6
The Rubella Virus causes the Rubella disease or the so-called German measles. It is a typical children’s disease and can get dangerous when first infection occurs after childhood. In this biobrick we include the sequence for the Rubella Glycoprotein E1 which is an envelope associated protein 1). To insert the sequence for rubella glycoprotein E1 into pSB1C3 we designed Gibson primers with compatible overhangs that also included the start codon ATG. The insert was amplified via PCR and then assembled with the digested pSB1C3 backbone using Gibson assembly.
To prove correct insertion of our fragment we did a test digest (Link Labjournal) and sent the whole plasmid for sequencing (GATC). Link to GeneBank file: BBa_K1621000.gb. Link to Registry: BBa_K1621000
