Difference between revisions of "Team:NYU Shanghai/Protocols"
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</tr> | </tr> | ||
</table> | </table> | ||
+ | </p> | ||
+ | <p> | ||
Lysozyme Solution (Desired Total Volume: 15mL)<br> | Lysozyme Solution (Desired Total Volume: 15mL)<br> | ||
<table border="1"> | <table border="1"> | ||
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</tr> | </tr> | ||
</table> | </table> | ||
+ | </p> | ||
+ | <p> | ||
Reagent Solution (Desired total volume: 22 mL)<br> | Reagent Solution (Desired total volume: 22 mL)<br> | ||
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<br><br>Materials | <br><br>Materials | ||
<li>Biomiga Miniprep Kit</li> | <li>Biomiga Miniprep Kit</li> | ||
− | + | </p> | |
+ | <p> | ||
+ | <br>Procedure | ||
<ol> | <ol> | ||
<li>Harvest the bacterial culture by centrifugation for 1 min at 10,000 rpm. Pour off the supernatant and blot the inverted tube on a paper towel to remove residue medium. Remove the residue medium completely. </li> | <li>Harvest the bacterial culture by centrifugation for 1 min at 10,000 rpm. Pour off the supernatant and blot the inverted tube on a paper towel to remove residue medium. Remove the residue medium completely. </li> | ||
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<br> | <br> | ||
<div id="PCRText" style="display:none"> | <div id="PCRText" style="display:none"> | ||
− | <p>These are the conditions we used to PCR the gBlocks received from IDT. We used <a href="http://www.snapgene.com"> Snapgene </a> to design the primers. We used <a href="https://www.neb.com/protocols/2013/12/13/pcr-using-q5-high-fidelity-dna-polymerase-m0491">Q5 High-Fideltiy Polymerase</a> from New England Biolabs. | + | <p>These are the conditions we used to PCR the gBlocks received from IDT. We used <a href="http://www.snapgene.com"> Snapgene </a> to design the primers. We used <a href="https://www.neb.com/protocols/2013/12/13/pcr-using-q5-high-fidelity-dna-polymerase-m0491">Q5 High-Fideltiy Polymerase</a>from New England Biolabs. |
<table border="1"> | <table border="1"> | ||
<tr> | <tr> |
Revision as of 02:55, 17 September 2015