Difference between revisions of "Team:Pretoria UP"

Revision as of 10:47, 17 September 2015

SYNCHRONIZED, CONDITIONAL, GENETIC CHEMOTAXIS PROGRAMMING

Project Description

The Pretoria UP iGEM team of 2015 have proposed a designed for intelligent motile bacteria based on a combination of DNA modules that confer different functions. The synchronous behaviour of the bacteria will be achieved through a quorum sensing module allowing the population to behave as a swarm instead of disparate units. A conditional response to an environmental signal is conferred through a post transcriptional control technique known as riboswitching, thus the bacterial swarm may recognise and respond to theophylline in an environment. Since the system makes use of several modular component, an AND gate is required to process the various signals which in turn would trigger a irreversible change in the bacterial chemotaxis. The irreversible change in behaviours is programmed by a DNA recombination switch which inverts the directionality of a promoter through the Cre-loxP pathway. We invite you to explore the various aspects of the on this site.

@@ Line 95: / Line 95: @@
 <p>
-We decided to improve the 2011 WITS-CSIR_SA “biotweet” project based on chemotaxis reversal in semi-solid media using riboswitches. The Pretoria_UP project “Switch coli” enhances several limitations on the original design, incorporating quorum sensing, an “AND” logic gate, alternative designs for a recombination-based toggle switch, and an irreversible reporter system for detecting chemicals of interest. The enhanced prototype would allow for motile bacteria to “tweet” information to a destination, and for a reply tweet with new information on the state of the destination to be sent back in a synchronized, conditional manner.
+The Pretoria UP iGEM team of 2015 have proposed a designed for intelligent motile bacteria based on a combination of DNA modules that confer different functions. The synchronous behaviour of the bacteria will be achieved through a quorum sensing module allowing the population to behave as a swarm instead of disparate units. A conditional response to an environmental signal is conferred through a post transcriptional control technique known as riboswitching, thus the bacterial swarm may recognise and respond to theophylline in an environment. Since the system makes use of several modular component, an AND gate is required to process the various signals which in turn would trigger a irreversible change in the bacterial chemotaxis. The irreversible change in behaviours is programmed by a DNA recombination switch which inverts the directionality of a promoter through the Cre-loxP pathway. We invite you to explore the various aspects of the on this site.
 </p>