Difference between revisions of "Team:ITB INDONESIA/idea/production-module"
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<h2>Production Module</h2> | <h2>Production Module</h2> | ||
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| + | <img src="https://static.igem.org/mediawiki/2015/e/ef/ITB_INDONESIA_IGEM2015_Production_module.png"> | ||
| + | <span class="caption">(Part submission tim ITB_Indonesia for production module, Rhamnolipid, Source: <a href="http://parts.igem.org/Part:BBa_K1685011">http://parts.igem.org/Part:BBa_K1685011</a>)</span> | ||
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There are two enzymes responsible for rhamnolipid production in <i>Pseudomonas aeruginosa</i>. The first enzyme, RhlA, catalyses dimerization of two hydroxyacyl (usually hydroxydecanoyl) chains that will constitute rhamnolipid’s lipid moiety, HAA. RhlB will catalyse the transfer of rhamnose and HAA forming mono-rhamnolipid. | There are two enzymes responsible for rhamnolipid production in <i>Pseudomonas aeruginosa</i>. The first enzyme, RhlA, catalyses dimerization of two hydroxyacyl (usually hydroxydecanoyl) chains that will constitute rhamnolipid’s lipid moiety, HAA. RhlB will catalyse the transfer of rhamnose and HAA forming mono-rhamnolipid. | ||
Revision as of 13:28, 17 September 2015
RhamCOLIpid
Engineered E. coli producing rhamnolipid for green enhanced oil recovery
Production Module
(Part submission tim ITB_Indonesia for production module, Rhamnolipid, Source: http://parts.igem.org/Part:BBa_K1685011)
There are two enzymes responsible for rhamnolipid production in Pseudomonas aeruginosa. The first enzyme, RhlA, catalyses dimerization of two hydroxyacyl (usually hydroxydecanoyl) chains that will constitute rhamnolipid’s lipid moiety, HAA. RhlB will catalyse the transfer of rhamnose and HAA forming mono-rhamnolipid.
We utilise The Biosurfactor biobrick (BBa_K653000) designed by Panama 2011 that encodes rhlA and rhlB. We put the biobrick under T7lac promoter with strong RBS, and double terminator.