Difference between revisions of "Team:BABS UNSW Australia/project/containment"
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<p> Due to the nature of our project, having several measures in place to ensure containment and biosafety is very important. We strongly felt that we should not experiment with introducing the invasion-listeriolysin devices into bacterial hosts until we had developed well characterised and effective safety checks. We focused on two key control measures: the Cre-lox recombination system which would delete genes encoding invasive proteins after entry into a cell, and a toxin-antitoxin system to contain bacteria to specific inducing agar plates and prevent horizontal gene transfer. <p/> | <p> Due to the nature of our project, having several measures in place to ensure containment and biosafety is very important. We strongly felt that we should not experiment with introducing the invasion-listeriolysin devices into bacterial hosts until we had developed well characterised and effective safety checks. We focused on two key control measures: the Cre-lox recombination system which would delete genes encoding invasive proteins after entry into a cell, and a toxin-antitoxin system to contain bacteria to specific inducing agar plates and prevent horizontal gene transfer. <p/> | ||
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| + | <h3>The Cre-Lox System</h3> | ||
Revision as of 17:10, 17 September 2015
#changethis
Introduction
Due to the nature of our project, having several measures in place to ensure containment and biosafety is very important. We strongly felt that we should not experiment with introducing the invasion-listeriolysin devices into bacterial hosts until we had developed well characterised and effective safety checks. We focused on two key control measures: the Cre-lox recombination system which would delete genes encoding invasive proteins after entry into a cell, and a toxin-antitoxin system to contain bacteria to specific inducing agar plates and prevent horizontal gene transfer.