Difference between revisions of "Team:WHU-Pharm/Results"
(Prototype team page) |
|||
(5 intermediate revisions by the same user not shown) | |||
Line 2: | Line 2: | ||
<html> | <html> | ||
− | < | + | <h1> Project Results</h1> |
− | <p> | + | <h2> Purification of Enzymes </h2> |
+ | <p> We have successfully purified the enzymes needed. Below is the SDS-PAGE result of most of these enzymes.</p> | ||
+ | <img src=https://static.igem.org/mediawiki/2015/b/bf/WHU-Pharm_SDSPAGE.jpeg /> | ||
+ | <p> From 1 to 10: Glk, Pgi, PfkA, FbaA, TpiA, Pgk, GpmA, Eno, PykF, GapA (M: protein ladder) | ||
− | < | + | <h2> Measurement of Enzyme Concentration</h2> |
− | < | + | <p> We used Pierce™ Microplate BCA Protein Assay Kit to measure concentrations of each enzyme.</p> |
− | + | <img src=https://static.igem.org/mediawiki/2015/thumb/5/56/WHU-Pharm_EnzymeConcentration.jpeg/459px-WHU-Pharm_EnzymeConcentration.jpeg /> | |
− | < | + | |
− | + | ||
− | + | ||
− | + | <h2><i>In Vitro</i> Expression</h2> | |
− | + | <p>We used protein expression kit by ThermoFisher Scientific © to express part Bba_J04450 along with the purified enzymes. Unfortunately, we failed to acquire a convincing result. This is possibly due to the inappropriate ratio of different components. We will continue to test different settings to test our designed glucose sensor. </p> | |
− | + | ||
− | + | ||
− | < | + | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | < | + | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
</div> | </div> | ||
</html> | </html> |
Latest revision as of 19:20, 17 September 2015
Project Results
Purification of Enzymes
We have successfully purified the enzymes needed. Below is the SDS-PAGE result of most of these enzymes.
![](https://static.igem.org/mediawiki/2015/b/bf/WHU-Pharm_SDSPAGE.jpeg)
From 1 to 10: Glk, Pgi, PfkA, FbaA, TpiA, Pgk, GpmA, Eno, PykF, GapA (M: protein ladder)
Measurement of Enzyme Concentration
We used Pierce™ Microplate BCA Protein Assay Kit to measure concentrations of each enzyme.
![](https://static.igem.org/mediawiki/2015/thumb/5/56/WHU-Pharm_EnzymeConcentration.jpeg/459px-WHU-Pharm_EnzymeConcentration.jpeg)
In Vitro Expression
We used protein expression kit by ThermoFisher Scientific © to express part Bba_J04450 along with the purified enzymes. Unfortunately, we failed to acquire a convincing result. This is possibly due to the inappropriate ratio of different components. We will continue to test different settings to test our designed glucose sensor.