Difference between revisions of "Team:Freiburg/Labjournals/Plasmids"

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<h1 class="sectionedit1">Plasmids</h1>
  
 
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<th class="col0"> No. </th><th class="col1"> Signature </th><th class="col2 rightalign">  C-term. Tag </th><th class="col3"> Antigen </th><th class="col4"> N-term. Tag </th><th class="col5"> Resistence </th><th class="col6"> Purpose </th>
 
<th class="col0"> No. </th><th class="col1"> Signature </th><th class="col2 rightalign">  C-term. Tag </th><th class="col3"> Antigen </th><th class="col4"> N-term. Tag </th><th class="col5"> Resistence </th><th class="col6"> Purpose </th>
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<td class="col0"> 1 </td><td class="col1"> pIG15_301 </td><td class="col2"> - </td><td class="col3"> Japanese Encephalitis Virus, <br/>
 
non-structural protein 1 </td><td class="col4"> - </td><td class="col5"> Ampicillin </td><td class="col6"> Cloning </td>
 
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<td class="col0"> 2 </td><td class="col1"> pIG15_401 </td><td class="col2"> - </td><td class="col3"> Dengue Virus, <br/>
 
non-structural protein 1 </td><td class="col4"> - </td><td class="col5"> Ampicillin </td><td class="col6"> Cloning </td>
 
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<td class="col0"> 3 </td><td class="col1"> pIG15_501 </td><td class="col2"> - </td><td class="col3"> St. Louis Encephalitis Virus, <br/>
 
non-structural protein 1 </td><td class="col4"> - </td><td class="col5"> Ampicillin </td><td class="col6"> Cloning </td>
 
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<td class="col0"> 4 </td><td class="col1"> pIG15_601 </td><td class="col2"> - </td><td class="col3"> Rubella Virus, <br/>
 
glycoprotein E1 </td><td class="col4"> - </td><td class="col5"> Ampicillin </td><td class="col6"> Cloning </td>
 
 
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<td class="col0"> 6 </td><td class="col1"> pRIG15_06 </td><td class="col2"> - </td><td class="col3"> Rubella Virus, <br/>
 
<td class="col0"> 6 </td><td class="col1"> pRIG15_06 </td><td class="col2"> - </td><td class="col3"> Rubella Virus, <br/>
 
glycoprotein E1 </td><td class="col4"> - </td><td class="col5"> Chloramphenicol </td><td class="col6"> Submission </td>
 
glycoprotein E1 </td><td class="col4"> - </td><td class="col5"> Chloramphenicol </td><td class="col6"> Submission </td>
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<td class="col0"> 7 </td><td class="col1"> pIG15_701 </td><td class="col2"> - </td><td class="col3"> Human Herpes Virus, <br/>
 
glycoprotein E </td><td class="col4"> - </td><td class="col5"> Ampicillin </td><td class="col6"> Cloning </td>
 
 
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<td class="col0"> 8 </td><td class="col1"> pRIG15_07 </td><td class="col2"> - </td><td class="col3"> Human Herpes Virus, <br/>
 
<td class="col0"> 8 </td><td class="col1"> pRIG15_07 </td><td class="col2"> - </td><td class="col3"> Human Herpes Virus, <br/>
 
glycoprotein E </td><td class="col4"> - </td><td class="col5"> Chloramphenicol </td><td class="col6"> Submission </td>
 
glycoprotein E </td><td class="col4"> - </td><td class="col5"> Chloramphenicol </td><td class="col6"> Submission </td>
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<td class="col0"> 9 </td><td class="col1"> pIG15_801 </td><td class="col2"> - </td><td class="col3"> Herpes simplex, <br/>
 
immunodominant epitope of glycoprotein G1 </td><td class="col4"> - </td><td class="col5"> Ampicillin </td><td class="col6"> Cloning </td>
 
 
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Revision as of 20:45, 17 September 2015

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Plasmids

No. Signature C-term. Tag Antigen N-term. Tag Resistence Purpose
5 pET_603 - Rubella Virus,
glycoprotein E1
10xHis Ampicillin Expression
6 pRIG15_06 - Rubella Virus,
glycoprotein E1
- Chloramphenicol Submission
8 pRIG15_07 - Human Herpes Virus,
glycoprotein E
- Chloramphenicol Submission
10 pIG15_804 10xHis Herpes simplex,
immunodominant epitope of glycoprotein G1
Spy Chloramphenicol Expression
11 pET_803 - Herpes simplex,
immunodominant epitope of glycoprotein G1
10xHis Ampicillin Expression
12 pET_804 6xHis Herpes simplex,
immunodominant epitope of glycoprotein G1
Spy Ampicillin Expression
13 pRIG15_08 - Herpes simplex,
immunodominant epitope of glycoprotein G1
- Chloramphenicol Submission
14 pIG15_901 - Yellow Fever Virus,
non-structural protein 1
- Chloramphenicol Cloning
15 pIG15_1001 - Hepatits C,
core protein
- Ampicillin Cloning
16 pET_1003 - Hepatits C,
core protein
10xHis Ampicillin Expression
17 pET_1004 6xHis Hepatits C,
core protein
Spy Ampicillin Expression
18 pIG15_1101 - Clostridium tetani,
TeNT_Hc
- Ampicillin Cloning
19 pIG15_1104 10xHis Clostridium tetani,
TeNT_Hc
Spy Chloramphenicol Expression
20 pIG15_1104_HA 10xHis_HA Clostridium tetani,
TeNT_Hc
Spy Chloramphenicol Expression
21 pIG15_1105 10xHis Clostridium tetani,
TeNT_Hc
Halo Chloramphenicol Expression
22 pET_1103 - Clostridium tetani,
TeNT_Hc
10xHis Ampicillin Expression
23 pET_1104 6xHis Clostridium tetani,
TeNT_Hc
Spy Ampicillin Expression
24 pET_1104_HA 6xHis_HA Clostridium tetani,
TeNT_Hc
Spy Ampicillin Expression
25 pET_1105 6xHis Clostridium tetani,
TeNT_Hc
Halo Ampicillin Expression
26 pRIG15_11 - Clostridium tetani,
TeNT_Hc
- Chloramphenicol Submission
27 pIG15_1201 - Human Papilloma Virus,
L1/L2
- Ampicillin Cloning
28 pIG15_1301 - anti-putative dihydroxyacid dehydratase,
ScvF
6xHis Ampicillin Cloning + Expression
29 pRIG15_13 - anti-putative dihydroxyacid dehydratase,
ScvF
- Chloramphenicol Submission
30 pIG15_1401 - anti-putative dimethyl sulfoxid reductase,
ScvF
6xHis Ampicillin Cloning + Expression
31 pIG15_1501 - Salmonella typhimurium,
putative dihydroxyacid dehydratase
6xHis Ampicillin Cloning + Expression
32 pIG15_1504 10xHis Salmonella typhimurium,
putative dihydroxyacid dehydratase
Spy Chloramphenicol Expression
33 pET_1504 6xHis Salmonella typhimurium,
putative dihydroxyacid dehydratase
Spy Ampicillin Expression
44 pIG15_1601 - Salmonella typhimurium,
putative dimethyl sulfoxid reductase
6xHis Ampicillin Cloning + Expression
45 pIG15_1701 - Human Immunodeficiency Virus,
immunodominant epitopes of the polyprotein tat/gag/pol/env
- Ampicillin Cloning
46 pIG15_1704 10xHis Human Immunodeficiency Virus,
immunodominant epitopes of the polyprotein tat/gag/pol/env
Spy Chloramphenicol Expression
47 pIG15_1704_HA 10xHis_HA Human Immunodeficiency Virus,
immunodominant epitopes of the polyprotein tat/gag/pol/env
Spy Chloramphenicol Expression
48 pET_1703 - Human Immunodeficiency Virus,
immunodominant epitopes of the polyprotein tat/gag/pol/env
10xHis Ampicillin Expression
49 pET_1703_HA HA Human Immunodeficiency Virus,
immunodominant epitopes of the polyprotein tat/gag/pol/env
10xHis Ampicillin Expression
50 pET_1704 6xHis Human Immunodeficiency Virus,
immunodominant epitopes of the polyprotein tat/gag/pol/env
Spy Ampicillin Expression
51 pET_1704_HA 6xHis_HA Human Immunodeficiency Virus,
immunodominant epitopes of the polyprotein tat/gag/pol/env
Spy Ampicillin Expression
52 pRIG15_17 - Human Immunodeficiency Virus,
immunodominant epitopes of the polyprotein tat/gag/pol/env
- Chloramphenicol Submission
53 pIG15_1801 - Treponema pallidum,
TpF1
- Ampicillin Cloning
54 pET_1803 - Treponema pallidum,
TpF1
10xHis Ampicillin Expression
55 pRIG15_18 - Treponema pallidum,
TpF1
- Chloramphenicol Submission
56 pILS_1 - GFP - Chloramphenicol InterLab Study
57 pILS_2 - GFP - Chloramphenicol InterLab Study
58 pILS_3 - GFP - Chloramphenicol InterLab Study
59 pQE_EGFP_His6 - EGFP 6xHis Ampicillin Expression
60 pIG15_204 10xHis EGFP Spy Chloramphenicol Expression
61 pIG15_204_HA 10xHis_HA EGFP Spy Chloramphenicol Expression
62 pIG15_205 10xHis EGFP Halo Chloramphenicol Expression
63 pIG15_205_HA 10xHis_HA EGFP Halo Chloramphenicol Expression
64 pSB1C3 - - - Chloramphenicol Cloning
65 pSB1A3 - - - Ampicillin Cloning
66 Bba_I13504 - GFP - Chloramphenicol InterLab Study
67 Bba_K823005 - Anderson promoter 01 - Chloramphenicol InterLab Study
68 Bba_K823008 - Anderson promoter 06 - Chloramphenicol InterLab Study
69 Bba_K823013 - Anderson promoter 17 - Chloramphenicol InterLab Study
70 Bba_I20270 - const. Promoter + GFP - Chloramphenicol InterLab Study
71 Bba_R0040 - Tet-repressible promoter - Chloramphenicol InterLab Study
72 pTurboYFP(Evrogen) - TurboYFP - Neomycin Cloning
73 pIG15_104 10xHis TurboYFP Spy Chloramphenicol Expression
74 pIG15_105 10xHis TurboYFP Halo Chloramphenicol Expression
75 pIG15_001 - - - Chloramphenicol Cloning + Expression
76 pIG15_001-A - - - Ampicillin Cloning + Expression
77 pIG15_002 - - - Chloramphenicol Cloning + Expression
78 pIG15_002-A - - - Ampicillin Cloning + Expression
79 pIG15_403 - Dengue Virus,
non-structural protein 1
10xHis Chloramphenicol Cloning + Expression
80 pIG15_403-A - Dengue Virus,
non-structural protein 1
10xHis Ampicillin Cloning + Expression
81 pIG15_803 - Herpes simplex,
immunodominant epitope of glycoprotein G1
10xHis Chloramphenicol Cloning + Expression
82 pIG15_803-A - Herpes simplex,
immunodominant epitope of glycoprotein G1
10xHis Ampicillin Cloning + Expression
83 pRARE2 - - - Chloramphenicol Cell-free expession system