Difference between revisions of "Team:Austin UTexas"

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<font color="000000">Our iGEM team at the University of Texas at Austin developed five individual projects inspired by members’ interests and concerns in synthetic biology, with a foundation of technical skills and lab experience built during a spring semester course. The projects our team members have devised focus on a multitude of topics, from attempts at improving the stability and efficiency of existing genetic machines, to identifying bacterial factories that can have ecological function. Our projects focused on improving and expanding on the existing microbial factories in E. coli include an attempt to optimize the ΔguaB pDCAF strain of <i>E. coli</i> (Quandt <i>et al.</i>, 2013) to discount nutrients provided by non-caffeine methylxanthines, and a project assessing the evolutionary stability of yellow fluorescent protein, enhanced yellow fluorescent protein, and super-folder yellow fluorescent protein. </font><br>
 
<font color="000000">Our iGEM team at the University of Texas at Austin developed five individual projects inspired by members’ interests and concerns in synthetic biology, with a foundation of technical skills and lab experience built during a spring semester course. The projects our team members have devised focus on a multitude of topics, from attempts at improving the stability and efficiency of existing genetic machines, to identifying bacterial factories that can have ecological function. Our projects focused on improving and expanding on the existing microbial factories in E. coli include an attempt to optimize the ΔguaB pDCAF strain of <i>E. coli</i> (Quandt <i>et al.</i>, 2013) to discount nutrients provided by non-caffeine methylxanthines, and a project assessing the evolutionary stability of yellow fluorescent protein, enhanced yellow fluorescent protein, and super-folder yellow fluorescent protein. </font><br>
 
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[[Image:2015_Austin_UTexas_homepage-BB.png|200px|thumb|left|<font size="2">Fluorescent protein-producing bacteria cultures grown over 10 days. One culture was grown each day by moving a small sample of the previous day's culture into new LB media.</font>]] 
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<b>References</b></br>
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<h6>Quandt, Erik M., et al. "Decaffeination and measurement of caffeine content by addicted Escherichia coli with a refactored N-demethylation operon from Pseudomonas putida CBB5." ACS synthetic biology 2.6 (2013): 301-307.</br></h6>
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<h6>Zhang, Hui-Juan, et al. "Biotransformation of the neonicotinoid insecticide thiacloprid by the bacterium Variovorax boronicumulans strain J1 and mediation of the major metabolic pathway by nitrile hydratase."Journal of agricultural and food chemistry 60.1 (2011): 153-159.</br></h6>
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<h6>Liu, Juan, et al. "An improved method for extracting bacteria from soil for high molecular weight DNA recovery and BAC library construction." The Journal of Microbiology 48.6 (2010): 728-733.</br></h6></p><p></p>
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<a href="https://2015.igem.org/Team:Austin_UTexas/Project/Problem"><font color="017e70"><b>GO TO PROJECT PAGE</b></font></a>
 
<a href="https://2015.igem.org/Team:Austin_UTexas/Project/Problem"><font color="017e70"><b>GO TO PROJECT PAGE</b></font></a>
 
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<h6>Quandt, Erik M., et al. "Decaffeination and measurement of caffeine content by addicted Escherichia coli with a refactored N-demethylation operon from Pseudomonas putida CBB5." ACS synthetic biology 2.6 (2013): 301-307.</br></h6>
 
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Revision as of 21:51, 17 September 2015

UT Austin iGEM 2015 Home

BREAKING IS BAD


Our iGEM team at the University of Texas at Austin developed five individual projects inspired by members’ interests and concerns in synthetic biology, with a foundation of technical skills and lab experience built during a spring semester course. The projects our team members have devised focus on a multitude of topics, from attempts at improving the stability and efficiency of existing genetic machines, to identifying bacterial factories that can have ecological function. Our projects focused on improving and expanding on the existing microbial factories in E. coli include an attempt to optimize the ΔguaB pDCAF strain of E. coli (Quandt et al., 2013) to discount nutrients provided by non-caffeine methylxanthines, and a project assessing the evolutionary stability of yellow fluorescent protein, enhanced yellow fluorescent protein, and super-folder yellow fluorescent protein.

Fluorescent protein-producing bacteria cultures grown over 10 days. One culture was grown each day by moving a small sample of the previous day's culture into new LB media.



GO TO PROJECT PAGE


CAFFEINATED COLI


redesign for greater stability

GO TO CAFFEINE PAGE


References
Quandt, Erik M., et al. "Decaffeination and measurement of caffeine content by addicted Escherichia coli with a refactored N-demethylation operon from Pseudomonas putida CBB5." ACS synthetic biology 2.6 (2013): 301-307.

INTERLAB STUDY & BREAKING IS BAD


what does BB have to do with interlab?

GO TO INTERLAB STUDY PAGE