Difference between revisions of "Team:NYU Shanghai/Parts"

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{{NYU_Shanghai}}
 
{{NYU_Shanghai}}
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<html>
 
<html>
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<script type="text/javascript">
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  window.onload = revealProject();
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</script>
  
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<h2> Part Documentation</h2>
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<style type="text/css">
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@font-face {
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</style>
  
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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<div id="overview">
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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  <h3>Parts</h3>
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  <h4>We’ve created three different types of colored E. Coli.</h4>
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  <img src="https://static.igem.org/mediawiki/2015/thumb/a/a5/NYU_Shanghai_realchromoproteins.png/560px-NYU_Shanghai_realchromoproteins.png" width="250" height="250" style="margin:0px 5px">
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  <p id="leftOverlay"><br><em>Chromoproteins</em><br><br>Pigmented compounds that absorb and reflect visible light.</p>
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  <img src="https://static.igem.org/mediawiki/2015/thumb/1/12/NYU_Shanghai_RFP.png/627px-NYU_Shanghai_RFP.png" width="250" height="250" style="margin:0px 5px">
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  <p id="rightOverlay"><br><em>Fluorescent Proteins</em><br><br>Pigmented compounds that absorb UV light and emit visible light.</p>
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  <img src="https://static.igem.org/mediawiki/2015/thumb/4/46/NYU_Shanghai_Luciferase2.png/597px-NYU_Shanghai_Luciferase2.png" width="250" height="250" style="margin:0px 5px">
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    <p id="luciferaseOverlay"><br><em>Luciferase Enzymes</em><br><br>Catalyzes conversion of luciferin to oxyluciferin. Energy is released in the form of the light.</p>
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</div>
  
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<div id="chromoproteins">
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<div class="highlightBox">
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  <h5>Chromoproteins</h5>
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<h4>Note</h4>
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  <img src="https://static.igem.org/mediawiki/2015/thumb/e/e7/NYU_Shanghai_Chromoprotein_construct.png/800px-NYU_Shanghai_Chromoprotein_construct.png" height="100">
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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  <div id="chromoText">
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    <p>We created constructs to express 3 different colors of chromoproteins: blue, lime, and yellow. These were taken from the registry, created in 2010 by the Cambridge iGEM team.</p>
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  </div>
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  <table border="1px">
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    <tr>
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      <td>BBa_K880005</td>
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      <td>promoter/rbs</td>
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    </tr>
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    <tr>
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      <td>BBa_K081005</td>
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      <td>promoter/rbs</td>
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    </tr>
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    <tr>
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      <td>BBa_K1333315</td>
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      <td>pBAD with araC</td>
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    </tr>
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    <tr>
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      <td>BBa_K592009</td>
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      <td>amilCP Blue</td>
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    </tr>
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    <tr>
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      <td>BBa_K1467201</td>
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      <td>Golden Gate compatible amilCP blue</td>
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    </tr>
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    <tr>
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      <td>BBa_K1033910</td>
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      <td>fwYellow Chromoprotein</td>
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    </tr>
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    <tr>
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      <td>BBa_K1033916</td>
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      <td>amajLime yellow-green chromoprotein</td>
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    </tr>
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    <tr>
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      <td>BBa_B0024</td>
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      <td>terminator</td>
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    </tr>
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    <tr>
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      <td>BBa_B0011</td>
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      <td>terminator</td>
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    </tr>
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  </table>
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  <br><br>
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  <img src="https://static.igem.org/mediawiki/2015/3/38/NYU_Shanghai_XJTLUchromo.png" height="100">
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  <div id="chromoText">
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    <p>XJTLU team used the T7 promoter in a bl21 E. Coli. This produced good blue and yellow color in our lab.</p>
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</div>
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<div id="fluorescentProteins">
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  <h5>Fluorescent Proteins</h5>
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  <img src="https://static.igem.org/mediawiki/2015/thumb/7/77/NYU_Shanghai_Fluorescent_Protein.png/800px-NYU_Shanghai_Fluorescent_Protein.png" height="100">
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  <div id="fluorescentText">
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    <p>We utilized generators to express 2 different colors of fluorescent proteins: RFP and GFP. These parts from the registry.</p>
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  </div>
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  <table border="1px">
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    <tr>
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      <td>BBa_J04450</td>
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      <td>RFP</td>
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    </tr>
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    <tr>
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      <td>BBa_J04451</td>
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      <td>RFP</td>
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    </tr>
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    <tr>
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      <td>BBa_KI13600</td>
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      <td>CFP</td>
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    </tr>
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    <tr>
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      <td>BBa_KE0040</td>
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      <td>GFP</td>
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    </tr>
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  </table>
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</div>
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<div id="luciferase">
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  <h5>Luciferase</h5>
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  <img src="https://static.igem.org/mediawiki/2015/thumb/d/d2/NYU_Shanghai_Luciferaseconstruct.png/800px-NYU_Shanghai_Luciferaseconstruct.png" height="100">
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  <div id="luciferaseText">
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    <p>We utilized generators to express 4 different bioluminescent colors: red, green, yellow, and orange.</p>
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  </div>
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  <table border="1px">
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    <tr>
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      <td>BBa_K325219</td>
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      <td>Red Firefly Luciferase and LRE (under pBAD)</td>
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    </tr>
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    <tr>
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      <td>BBa_K325209</td>
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      <td>Green Firefly Luciferase and LRE (under pBAD)</td>
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    </tr>
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    <tr>
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      <td>BBa_K325259</td>
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      <td>Yellow Firefly Luciferase (under pBAD)</td>
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    </tr>
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    <tr>
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      <td>BBa_K325218</td>
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      <td>Orange Firefly Luciferase (under pBAD)</td>
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    </tr>
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    <tr>
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      <td>BBa_K1486022</td>
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      <td>Renilla Luciferase</td>
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    </tr>
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    <tr>
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      <td>BBa_K325108</td>
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      <td>EPIC Firefly Luciferase (under pBAD)</td>
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    </tr>
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  </table>
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</div>
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<div id="oscillatingLuciferase">
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  <h5>Oscillating Luciferase</h5>
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  <img src="https://static.igem.org/mediawiki/2015/thumb/f/f6/NYU_Shanghai_Oscillating_Luciferase.png/800px-NYU_Shanghai_Oscillating_Luciferase.png" height="240">
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  <p>To create a natural beat generator, we created a light oscillating system. Unfortunately, the time scale was far too long for any practical beat generator (hypothetical 40min per beat). See <a href="https://2012.igem.org/Team:Fudan_Lux/biowave" style="color: #6db; text-decoration: none;">Fudan 2012 Biowave</a> for more information.</p>
 
</div>
 
</div>
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<h4>Adding parts to the registry</h4>
 
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
 
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
 
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<h4>What information do I need to start putting my parts on the Registry?</h4>
 
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<p>The information needed to initially create a part on the Registry is:</p>
 
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<ul>
 
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<li>Part Name</li>
 
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<li>Part type</li>
 
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<li>Creator</li>
 
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<li>Sequence</li>
 
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<li>Short Description (60 characters on what the DNA does)</li>
 
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<li>Long Description (Longer description of what the DNA does)</li>
 
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<li>Design considerations</li>
 
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</ul>
 
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<p>
 
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
 
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<h4>Inspiration</h4>
 
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
 
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
 
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<ul>
 
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
 
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
 
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
 
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</ul>
 
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<h4>Part Table </h4>
 
 
</html>
 
</html>
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<groupparts>iGEM015 Example</groupparts>
 
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<html>
 
  
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{{NYU_Shanghai/Sponsors}}
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</div>
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</html>
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Latest revision as of 23:08, 17 September 2015

Parts

We’ve created three different types of colored E. Coli.


Chromoproteins

Pigmented compounds that absorb and reflect visible light.


Fluorescent Proteins

Pigmented compounds that absorb UV light and emit visible light.


Luciferase Enzymes

Catalyzes conversion of luciferin to oxyluciferin. Energy is released in the form of the light.

Chromoproteins

We created constructs to express 3 different colors of chromoproteins: blue, lime, and yellow. These were taken from the registry, created in 2010 by the Cambridge iGEM team.

BBa_K880005 promoter/rbs
BBa_K081005 promoter/rbs
BBa_K1333315 pBAD with araC
BBa_K592009 amilCP Blue
BBa_K1467201 Golden Gate compatible amilCP blue
BBa_K1033910 fwYellow Chromoprotein
BBa_K1033916 amajLime yellow-green chromoprotein
BBa_B0024 terminator
BBa_B0011 terminator


XJTLU team used the T7 promoter in a bl21 E. Coli. This produced good blue and yellow color in our lab.

Fluorescent Proteins

We utilized generators to express 2 different colors of fluorescent proteins: RFP and GFP. These parts from the registry.

BBa_J04450 RFP
BBa_J04451 RFP
BBa_KI13600 CFP
BBa_KE0040 GFP
Luciferase

We utilized generators to express 4 different bioluminescent colors: red, green, yellow, and orange.

BBa_K325219 Red Firefly Luciferase and LRE (under pBAD)
BBa_K325209 Green Firefly Luciferase and LRE (under pBAD)
BBa_K325259 Yellow Firefly Luciferase (under pBAD)
BBa_K325218 Orange Firefly Luciferase (under pBAD)
BBa_K1486022 Renilla Luciferase
BBa_K325108 EPIC Firefly Luciferase (under pBAD)
Oscillating Luciferase

To create a natural beat generator, we created a light oscillating system. Unfortunately, the time scale was far too long for any practical beat generator (hypothetical 40min per beat). See Fudan 2012 Biowave for more information.