Difference between revisions of "Team:NYU Shanghai/Protocols"

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     <h6><font color="#d66">Building our Construct</font></h6>
 
     <h6><font color="#d66">Building our Construct</font></h6>
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     <h6><font color="#d66">Expressing XJTLU's Construct</font></h6>
 
     <h6><font color="#d66">Expressing XJTLU's Construct</font></h6>
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Revision as of 00:26, 18 September 2015

Protocols

We built our constructs from pre-made biobrick parts. Our overall conclusion is that 3A assembly is generally inefficient, and an insufficient method for adding small parts (such as a terminator) to a larger construction within pSB1C3. We learned that ratios were extremely important in the process of 3A Assembly, and we made a summary sheet of the equations we used in pre-digest and pre-ligation that accounts for digest dilution and amount needed to ensure results are seen on a gel, not just ligation ratios. We wished we used gibson assembly.


Making Color

Recipes

3A Assembly

Calculations (pdf)