Difference between revisions of "Team:Brasil-USP/Notebook/Calendar"
Line 5: | Line 5: | ||
<div class="jamboreh"> | <div class="jamboreh"> | ||
− | + | <h1>June</h1> | |
Line 19: | Line 19: | ||
</tr> <tr> | </tr> <tr> | ||
<td valign=top> 01/06 | <td valign=top> 01/06 | ||
− | + | ||
− | - Agarose gel (RBS_hokD)<br><br> | + | <br> |
− | - Gel purification (RBS_hokD)<br><br> | + | - Agarose gel (RBS_hokD) <br><br> |
+ | - Gel purification (RBS_hokD) <br><br> | ||
- Ligation 2X_RBS_hokD (RBS_hokD + RBS_hokD) <br><br> | - Ligation 2X_RBS_hokD (RBS_hokD + RBS_hokD) <br><br> | ||
- EXSP Digestion (P0040, Pl_RBS, GFP_R0440) <br><br> | - EXSP Digestion (P0040, Pl_RBS, GFP_R0440) <br><br> | ||
Line 29: | Line 30: | ||
- Interlab gel purification and ligation <br><br> | - Interlab gel purification and ligation <br><br> | ||
<br> | <br> | ||
+ | |||
+ | |||
+ | |||
</td> | </td> | ||
<td valign=top> 02/06 | <td valign=top> 02/06 | ||
− | + | <br> | |
+ | - Transformation Ptet_I13507<br><br> | ||
+ | - Agarose gel (Pl_RBS, GFP_P0440)<br><br> | ||
+ | - Gel purification (Pl_RBS, GFP_P0440)<br><br> | ||
+ | - Ligation Pl_P0440 (Pl_RBS + GFP_P0440)<br><br> | ||
+ | - Interlab transformation (with wrong antibiotic ampicillin - is possible found in registry the same parts in another backbone BBa_J61002)<br><br> | ||
+ | <br> | ||
</td> | </td> | ||
<td valign=top align=left> 03/06 | <td valign=top align=left> 03/06 | ||
− | + | ||
− | + | <br> | |
+ | - Transformation (Pr_RBS, 2X_RBS_hokD)<br><br> | ||
+ | - EXSP digestion (P0440, R0040)<br><br> | ||
+ | - Transformation Pl_R0440 (Pl_RBS + GFP_P0440)<br><br> | ||
+ | - Inoculum (Pt_I13507)<br><br> | ||
+ | <br> | ||
</td> | </td> | ||
<td valign=top align=left> 04/06 | <td valign=top align=left> 04/06 | ||
+ | <br> | ||
+ | - Agarose gel (P0440, R0040)<br><br> | ||
+ | - Gel purification (P0440, R0040)<br><br> | ||
+ | - Ligation tet_Ptet (P0440 + R0040)<br><br> | ||
+ | - Inoculum (2X_RBS_hokD)<br><br> | ||
+ | - Miniprep (Pt_I13507)<br><br> | ||
+ | <br> | ||
</td> | </td> | ||
<td valign=top align=left> 05/06 | <td valign=top align=left> 05/06 | ||
+ | <br> | ||
+ | - Inoculum (R0080, K91403)<br><br> | ||
+ | - EXSP digestion (tet_Term, Pt_E0840)<br><br> | ||
+ | - Miniprep (2X_RBS_hokD)<br><br> | ||
+ | <br> | ||
+ | |||
+ | |||
</td> | </td> | ||
<td valign=top align=left> 06/06 | <td valign=top align=left> 06/06 | ||
− | + | <br> | |
+ | - NotI digestion (2X_RBS_hokD, Pr_RBS, Pa_RBS)<br><br> | ||
+ | - Transformation (tet_Ptet)<br><br> | ||
+ | - Agarose gel (2X_RBS_hokD) – positive results<br><br> | ||
+ | - Agarose gel (tet_Term, Pt_E0840)<br><br> | ||
+ | - EXSP digestion (Pt_E0480)<br><br> | ||
+ | - Inoculum (Pl_P0440)<br><br> | ||
+ | - NotI digestion (Pt_I13507) – positive results <br><br> | ||
+ | <br> | ||
+ | |||
+ | </td> | ||
<td valign=top align=left> 07/06 | <td valign=top align=left> 07/06 | ||
+ | <br> | ||
+ | - Agarose gel (Pr_RBS, Pa_RBS, Pt_E0480) – positive results for Pr_RBS and Pt_E0840)<br><br> | ||
+ | - EXSP digestion (2X_RBS_hokD, B0015)<br><br> | ||
+ | - Ligation Term_E0840 (Pt_E0840 + tet_Term)<br><br> | ||
+ | - Inoculum (tet_Ptet)<br><br> | ||
+ | - Transformation (Term_E0840)<br><br> | ||
+ | - Miniprep (Pl_P0440)<br><br> | ||
+ | - Not digestion (Pl_P0440) – positive results<br><br> | ||
+ | - EXSP digestion (Pl_P0440, Pt_I13507)<br><br> | ||
+ | - Inoculum (GFP_P0440)<br><br> | ||
+ | <br> | ||
</td> | </td> | ||
</tr> <tr> | </tr> <tr> | ||
<td valign=top align=left> 08/06</td> | <td valign=top align=left> 08/06</td> | ||
+ | <br> | ||
+ | - Agarose gel (2X_RBS_hokD, B0015) – B0015 didn’t digest<br><br> | ||
+ | - Gel purification (2X_RBS_hokD)<br><br> | ||
+ | - EXSP digestion (B0015)<br><br> | ||
+ | - Miniprep (tet_Ptet)<br><br> | ||
+ | - NotI digestion (tet_Ptet)<br><br> | ||
+ | - Agarose gel (tet_Ptet, B0015) – positive results<br><br> | ||
+ | - Gel digestion (B0015)<br><br> | ||
+ | - Ligation hokD_Term (2X_RBS_hokD + B0015)<br><br> | ||
+ | - Ligation Pl_I13507 (Pl_P0440 + Pt_I13507)<br><br> | ||
+ | - Miniprep (GFP_P0440)<br><br> | ||
+ | - EXSP digestion (GFP_P0440)<br><br> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <br> | ||
<td > 09/06 | <td > 09/06 | ||
− | + | <br> | |
− | + | - Agarose gel and gel purification (GFP_P0440)<br><br> | |
+ | - Ligation GFP_I13507 (GFP_P0440 + Pt_I13507)<br><br> | ||
+ | - Transformation (Pl_I13507)<br><br> | ||
+ | <br> | ||
</td> | </td> | ||
<td valign=top align=left> 10/06 | <td valign=top align=left> 10/06 | ||
− | + | <br> | |
− | + | - Transformation (hokD_Term)<br><br> | |
− | + | - Inoculum (Pl_I13507)<br><br> | |
+ | - Transformation (GFP_I13507)<br><br> | ||
+ | <br> | ||
+ | |||
<td valign=top align=left> 11/06 | <td valign=top align=left> 11/06 | ||
− | + | <br> | |
− | + | - Miniprep (Pl_I13507)<br><br> | |
− | + | <br> | |
</td> | </td> | ||
+ | |||
<td valign=top align=left> 12/06 | <td valign=top align=left> 12/06 | ||
− | |||
<td valign=top align=left> 13/06 | <td valign=top align=left> 13/06 | ||
− | + | <br> | |
− | + | - Inoculum (GFP_I13507)<br><br> | |
− | + | - NotI digestion (Pl_I13507)<br><br> | |
+ | - Agarose gel (Pl_I13507) – negative results<br><br> | ||
+ | <br> | ||
</td> | </td> | ||
− | + | ||
− | + | <td valign=top align=left> 14/06 | |
− | + | ||
+ | <br> | ||
+ | - Miniprep (GFP_I13507)<br><br> | ||
+ | - NotI digestion (GFP_I13507) – negative results<br><br> | ||
+ | - EXSP digestion (Pl_P0440, Pt_I13507)<br><br> | ||
+ | - Inoculum (GFP_P0440, Pt_I13507)<br><br> | ||
+ | <br> | ||
<tr> | <tr> | ||
<td valign=top align=left> 15/06 | <td valign=top align=left> 15/06 | ||
− | + | <br> | |
− | + | - Agarose gel (Pl_P0440, Pt_I13507)<br><br> | |
+ | - Gel purification (Pl_P0440, Pt_I13507)<br><br> | ||
+ | - Ligation Pl_I13507 (Pl_P0440 + Pt_I13507)<br><br> | ||
+ | - Miniprep (GFP_P0440, Pt_I13507)<br><br> | ||
+ | - EXSP digestion (GFP_P0440, Pt_I13507)<br><br> | ||
+ | <br> | ||
+ | |||
</td> | </td> | ||
<td valign=top align=left> 16/06 | <td valign=top align=left> 16/06 | ||
− | + | <br> | |
+ | - Agarose gel and gel purification (GFP_P0440, Pt_I13507)<br><br> | ||
+ | - Ligation GFP_I13507 (GFP_P0440 + Pt_I13507)<br><br> | ||
+ | - Transformation (Pl_I13507)<br><br> | ||
+ | <br> | ||
+ | |||
<td valign=top align=left> 17/06 | <td valign=top align=left> 17/06 | ||
− | + | <br> | |
− | + | - Transformation (GFP_I13507)<br><br> | |
− | + | - Inoculum (Pt_Term, Pl_I1350)<br><br> | |
+ | <br> | ||
+ | |||
+ | |||
</td> | </td> | ||
<td valign=top align=left> 18/06 | <td valign=top align=left> 18/06 | ||
− | + | <br> | |
− | + | - Miniprep (Pt_Term, Pl_I13507)<br><br> | |
− | + | - NotI digestion (Pt_Term, Pl_I13507)<br><br> | |
+ | - Agarose gel (Pt_Term, Pl_I13507) – positive results for Pt_Term<br><br> | ||
+ | <br> | ||
<td valign=top align=left> 19/06 | <td valign=top align=left> 19/06 | ||
− | + | ||
− | + | ||
</td> | </td> | ||
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<td valign=top align=left> 21/06 | <td valign=top align=left> 21/06 | ||
− | + | <br> | |
− | + | - Inoculum (GFP_I13507, Pl_I13507)<br><br> | |
− | + | - New interlab transformations<br><br> | |
+ | <br> | ||
</td> | </td> | ||
</tr> | </tr> | ||
<td valign=top align=left> 22/06 | <td valign=top align=left> 22/06 | ||
− | + | <br> | |
− | + | - Miniprep (GFP_I13507, Pl_I13507)<br><br> | |
− | + | - NotI digestion (GFP_I13507, Pl_I13507)<br><br> | |
+ | - EXSP digestion (Pr_RBS, R0080)<br><br> | ||
+ | - Agarose gel (GFP_I13507, Pl_I13507, Pr_RBS)<br><br> | ||
+ | - Gel purification (Pr_RBS)<br><br> | ||
+ | - Inoculum (GFP_I13507, Pl_I13507)<br><br> | ||
+ | - Interlab inocula<br><br> | ||
+ | <br> | ||
<td valign=top align=left> 23/06 | <td valign=top align=left> 23/06 | ||
− | + | <br> | |
− | + | - Agarose gel (R0080)<br><br> | |
+ | - Miniprep (GFP_I13507, Pl_I13507)<br><br> | ||
+ | - NotI digestion (GFP_I13507, Pl_I13507)<br><br> | ||
+ | - Agarose gel (GFP_I13507, Pl_I13507) – Positive results for GFP_I13507<br><br> | ||
+ | - EXSP digestion (GFP_I13507)<br><br> | ||
+ | - Interlab miniprep and restriction analysis<br><br> | ||
+ | <br> | ||
+ | |||
</td> | </td> | ||
<td valign=top align=left> 24/06 | <td valign=top align=left> 24/06 | ||
− | + | <br> | |
− | + | - Miniprep (B0015, Pt_P0440)<br><br> | |
+ | - EXSP digestion (2X_RBS_hokD, B0015)<br><br> | ||
+ | - Transformation (Pa_RBS)<br><br> | ||
+ | - Agarose gel of restriction analysis: devices confirmed<br><br> | ||
+ | <br> | ||
+ | |||
<td valign=top align=left> 25/06 | <td valign=top align=left> 25/06 | ||
− | + | ||
− | + | <br> | |
+ | - agarose gel (2X_RBS_hokD, B0015) - B0015 did not digest<br><br> | ||
+ | <br> | ||
</td> | </td> | ||
<td valign=top align=left> 26/06 | <td valign=top align=left> 26/06 | ||
− | + | <br> | |
− | + | - Agarose gel and gel purification (GFP_I13507)<br><br> | |
− | + | - Ligation Pr_I13507 (Pr_RBS + GFP_I13507)<br><br> | |
+ | <br> | ||
<td valign=top align=left> 27/06 | <td valign=top align=left> 27/06 | ||
− | + | <br> | |
− | + | - EXSP digestion (Pl_RBS, Pt_Term)<br><br> | |
+ | - Transformation (Pr_I13507, Pa_RBS)<br><br> | ||
+ | <br> | ||
</td> | </td> | ||
<td valign=top align=left> 28/06 | <td valign=top align=left> 28/06 | ||
− | + | <br> | |
− | + | - Agarose gel (Pl_RBS, Pt_Term)<br><br> | |
− | + | ligation Pr_I13507(Pr_RBS + Pt_Term)<br><br> | |
+ | <br> | ||
</td> | </td> | ||
<tr> | <tr> | ||
Line 146: | Line 267: | ||
<td valign=top align=left> 29/06 | <td valign=top align=left> 29/06 | ||
− | + | <br> | |
− | + | - EXSP digestion (B0015, 2X_RBS_hokD)<br><br> | |
+ | - agarose gel (B0015, 2X_RBS_hokD) - 2X_RBS_hokD didn’t digest<br><br> | ||
+ | - EXSP digestion overnight (2X_RBS_hokD)<br><br> | ||
+ | - transformation (Pr_Term, Pl_RBS)<br><br> | ||
+ | <br> | ||
</td> | </td> | ||
<td valign=top align=left> 30/06 | <td valign=top align=left> 30/06 | ||
− | + | <br> | |
− | + | - inoculum (Pl_RBS, Pa_RBS)<br><br> | |
+ | - agarose gel (B0015, 2X_RBS_hokD) - Digestion worked!<br><br> | ||
+ | - ligation 2X_hokD_Term (B0015 + 2X_RBS_hokD) P0440_hokD(P0440_term + 2X_RBS_hokD)<br><br> | ||
+ | <br> | ||
+ | |||
</td> | </td> | ||
Line 161: | Line 290: | ||
</table> | </table> | ||
+ | |||
Revision as of 03:58, 18 September 2015
Notebook
Calendar
![](https://static.igem.org/mediawiki/2015/7/7d/Team-Brasil-USP_MainLogo.png)
June
Monday | Tuesday | Wednesday | Thursday | Friday | Saturday | Sunday |
01/06
- Agarose gel (RBS_hokD) - Gel purification (RBS_hokD) - Ligation 2X_RBS_hokD (RBS_hokD + RBS_hokD) - EXSP Digestion (P0040, Pl_RBS, GFP_R0440) - Agarose gel (P0040) - Gel purification (P0040) - Ligation Pt_I13507(P0040 + I13507) - Interlab gel purification and ligation |
02/06
- Transformation Ptet_I13507 - Agarose gel (Pl_RBS, GFP_P0440) - Gel purification (Pl_RBS, GFP_P0440) - Ligation Pl_P0440 (Pl_RBS + GFP_P0440) - Interlab transformation (with wrong antibiotic ampicillin - is possible found in registry the same parts in another backbone BBa_J61002) |
03/06
- Transformation (Pr_RBS, 2X_RBS_hokD) - EXSP digestion (P0440, R0040) - Transformation Pl_R0440 (Pl_RBS + GFP_P0440) - Inoculum (Pt_I13507) |
04/06
- Agarose gel (P0440, R0040) - Gel purification (P0440, R0040) - Ligation tet_Ptet (P0440 + R0040) - Inoculum (2X_RBS_hokD) - Miniprep (Pt_I13507) |
05/06
- Inoculum (R0080, K91403) - EXSP digestion (tet_Term, Pt_E0840) - Miniprep (2X_RBS_hokD) |
06/06
- NotI digestion (2X_RBS_hokD, Pr_RBS, Pa_RBS) - Transformation (tet_Ptet) - Agarose gel (2X_RBS_hokD) – positive results - Agarose gel (tet_Term, Pt_E0840) - EXSP digestion (Pt_E0480) - Inoculum (Pl_P0440) - NotI digestion (Pt_I13507) – positive results |
07/06
- Agarose gel (Pr_RBS, Pa_RBS, Pt_E0480) – positive results for Pr_RBS and Pt_E0840) - EXSP digestion (2X_RBS_hokD, B0015) - Ligation Term_E0840 (Pt_E0840 + tet_Term) - Inoculum (tet_Ptet) - Transformation (Term_E0840) - Miniprep (Pl_P0440) - Not digestion (Pl_P0440) – positive results - EXSP digestion (Pl_P0440, Pt_I13507) - Inoculum (GFP_P0440) |
08/06 | 09/06
- Agarose gel and gel purification (GFP_P0440) - Ligation GFP_I13507 (GFP_P0440 + Pt_I13507) - Transformation (Pl_I13507) |
10/06
- Transformation (hokD_Term) - Inoculum (Pl_I13507) - Transformation (GFP_I13507) | 11/06
- Miniprep (Pl_I13507) |
12/06 | 13/06
- Inoculum (GFP_I13507) - NotI digestion (Pl_I13507) - Agarose gel (Pl_I13507) – negative results |
14/06
- Miniprep (GFP_I13507) - NotI digestion (GFP_I13507) – negative results - EXSP digestion (Pl_P0440, Pt_I13507) - Inoculum (GFP_P0440, Pt_I13507) |
15/06
- Agarose gel (Pl_P0440, Pt_I13507) - Gel purification (Pl_P0440, Pt_I13507) - Ligation Pl_I13507 (Pl_P0440 + Pt_I13507) - Miniprep (GFP_P0440, Pt_I13507) - EXSP digestion (GFP_P0440, Pt_I13507) |
16/06
- Agarose gel and gel purification (GFP_P0440, Pt_I13507) - Ligation GFP_I13507 (GFP_P0440 + Pt_I13507) - Transformation (Pl_I13507) | 17/06
- Transformation (GFP_I13507) - Inoculum (Pt_Term, Pl_I1350) |
18/06
- Miniprep (Pt_Term, Pl_I13507) - NotI digestion (Pt_Term, Pl_I13507) - Agarose gel (Pt_Term, Pl_I13507) – positive results for Pt_Term | 19/06 | 20/06
| 21/06
- Inoculum (GFP_I13507, Pl_I13507) - New interlab transformations |
22/06
- Miniprep (GFP_I13507, Pl_I13507) - NotI digestion (GFP_I13507, Pl_I13507) - EXSP digestion (Pr_RBS, R0080) - Agarose gel (GFP_I13507, Pl_I13507, Pr_RBS) - Gel purification (Pr_RBS) - Inoculum (GFP_I13507, Pl_I13507) - Interlab inocula | 23/06
- Agarose gel (R0080) - Miniprep (GFP_I13507, Pl_I13507) - NotI digestion (GFP_I13507, Pl_I13507) - Agarose gel (GFP_I13507, Pl_I13507) – Positive results for GFP_I13507 - EXSP digestion (GFP_I13507) - Interlab miniprep and restriction analysis |
24/06
- Miniprep (B0015, Pt_P0440) - EXSP digestion (2X_RBS_hokD, B0015) - Transformation (Pa_RBS) - Agarose gel of restriction analysis: devices confirmed | 25/06
- agarose gel (2X_RBS_hokD, B0015) - B0015 did not digest |
26/06
- Agarose gel and gel purification (GFP_I13507) - Ligation Pr_I13507 (Pr_RBS + GFP_I13507) | 27/06
- EXSP digestion (Pl_RBS, Pt_Term) - Transformation (Pr_I13507, Pa_RBS) |
28/06
- Agarose gel (Pl_RBS, Pt_Term) ligation Pr_I13507(Pr_RBS + Pt_Term) |
29/06
- EXSP digestion (B0015, 2X_RBS_hokD) - agarose gel (B0015, 2X_RBS_hokD) - 2X_RBS_hokD didn’t digest - EXSP digestion overnight (2X_RBS_hokD) - transformation (Pr_Term, Pl_RBS) |
30/06
- inoculum (Pl_RBS, Pa_RBS) - agarose gel (B0015, 2X_RBS_hokD) - Digestion worked! - ligation 2X_hokD_Term (B0015 + 2X_RBS_hokD) P0440_hokD(P0440_term + 2X_RBS_hokD) |
31/06
|