Difference between revisions of "Team:BGU Israel/Experiments"

 
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<h2>Experiments &amp; Protocols</h2>
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<p>Describe the experiments, research and protocols you used in your iGEM project.</p>
 
  
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<h5>What should this page contain?</h5>
 
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<ul>
 
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<li> Protocols </li>
 
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<li> Experiments </li>
 
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<li>Documentation of the development of your project </li>
 
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</ul>
 
  
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<h2> Those are the protocols we used throughout the summer:</h2>
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        <table width=90% align=center class="parts">
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<tr><br><br><br>
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<td><h3><b>Protocol</b></h3></td>
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<td><h3><b>Link to Protocol</b></h3></td>
  
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<h4>Inspiration</h4>
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</tr>
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<ul>
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<li><a href="https://2014.igem.org/Team:Colombia/Protocols">2014 Colombia </a></li>
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<li><a href="https://2014.igem.org/Team:Imperial/Protocols">2014 Imperial </a></li>
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<li><a href="https://2014.igem.org/Team:Caltech/Project/Experiments">2014 Caltech </a></li>
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</ul>
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</div>  <br /><br /><br />  <br /><br /><br />
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<tr>
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<td>Bacterial transformation</td>
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<td><a href="https://static.igem.org/mediawiki/2015/1/1e/Hit_competent_cells_brochure.pdf"> cell transformation</a></td>
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</tr>
  
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<tr>
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<td>Gel Extraction</td>
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<td><a href="https://static.igem.org/mediawiki/2015/4/4a/Gel_extraction.pdf"> Gel Extraction </a></td>
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</tr>
  
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</div>
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</div>
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<tr>
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</div>
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<td> Ligation</td>
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<td><a href="https://static.igem.org/mediawiki/2015/9/9d/Ligation.pdf"> Ligation </a></td>
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</tr>
  
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<tr>
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<td>Midiprep  </td>
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<td><a href="https://static.igem.org/mediawiki/2015/4/47/Midiprep.pdf"> Midiprep </a></td>
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</tr>
  
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<tr>
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<td>Miniprep</td>
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<td><a href="https://static.igem.org/mediawiki/2015/d/dd/Miniprep.pdf"> Miniprep</a></td>
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</tr>
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<tr>
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<td>PCR_hifi</td>
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<td><a href="https://static.igem.org/mediawiki/2015/9/9e/PCR_hifi.pdf"> PCR_hifi</a></td>
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</tr>
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<tr>
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<td>PCR Purification</td>
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<td><a href="https://static.igem.org/mediawiki/2015/b/b7/Vilnius15_PCRPurification.pdf">PCR Purification</a></td>
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</tr>
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<tr>
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<td>AGILENT AAV helper system</td>
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<td><a href="https://static.igem.org/mediawiki/2015/e/e7/Virus.pdf"> Virus</a></td>
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</tr>
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<tr>
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<td>AAV production</td>
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<td><a href="https://static.igem.org/mediawiki/2015/d/dd/AAV_production_Protocol.pdf"> AAV_production_Protocol</a></td>
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</tr>
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<tr>
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<td>CaP transfection</td>
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<td><a href="https://static.igem.org/mediawiki/2015/8/89/CaP_transfection.pdf">CaP_transfection</a></td>
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</tr>
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<tr>
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<td>EZ-RNA Toal RNA isolation</td>
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<td><a href="https://static.igem.org/mediawiki/2015/b/b0/EZ-RNA_Toal_RNA_isolation_Kit.pdf">EZ-RNA_Toal_RNA_isolation</a></td>
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</tr>
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<tr>
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<td>High Capacity cDNA reverse transcription</td>
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<td><a href="https://static.igem.org/mediawiki/2015/f/fd/High_Capacity_cDNA_reverse_transcription_kit.pdf">High_Capacity_cDNA_reverse_transcription</a></td>
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</tr>
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<tr>
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<td>Gel Preparation</td>
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<td><a href="https://static.igem.org/mediawiki/2015/c/ca/Gel_preparation.pdf"> Gel Preparation </a></td>
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</tr>
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            <tr>
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<td>Preparation of anibiotics stocks</td>
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<td><a href="https://static.igem.org/mediawiki/2015/c/cf/Preparation_of_anibiotics_stocks.pdf"> Preparation of anibiotics stocks
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</a></td>
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</tr>
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            <tr>
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<td>Preparation of LB Agar plates</td>
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<td><a href="https://static.igem.org/mediawiki/2015/f/f4/Preparation_of_LB_Agar_plates.pdf"> Preparation of LB Agar plates </a></td>
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</tr>
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            <tr>
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<td>Preparation of liquid LB (for bacteria growth)</td>
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<td><a href="https://static.igem.org/mediawiki/2015/6/65/Preparation_of_liquid_LB.pdf"> Preparation of liquid LB (for bacteria growth) </a></td>
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</tr>
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            <tr>
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<td>IF staining protocol</td>
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<td><a href="https://static.igem.org/mediawiki/2015/7/73/IF_staining_protocol.pdf"> IF_staining_protocol </a></td>
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</tr>
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</table>
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Latest revision as of 09:16, 18 September 2015

Team:BGU Israel



Those are the protocols we used throughout the summer:




Protocol

Link to Protocol
Bacterial transformation cell transformation
Gel Extraction Gel Extraction
Ligation Ligation
Midiprep Midiprep
Miniprep Miniprep
PCR_hifi PCR_hifi
PCR Purification PCR Purification
AGILENT AAV helper system Virus
AAV production AAV_production_Protocol
CaP transfection CaP_transfection
EZ-RNA Toal RNA isolation EZ-RNA_Toal_RNA_isolation
High Capacity cDNA reverse transcription High_Capacity_cDNA_reverse_transcription
Gel Preparation Gel Preparation
Preparation of anibiotics stocks Preparation of anibiotics stocks
Preparation of LB Agar plates Preparation of LB Agar plates
Preparation of liquid LB (for bacteria growth) Preparation of liquid LB (for bacteria growth)
IF staining protocol IF_staining_protocol