Difference between revisions of "Team:BABS UNSW Australia/Protocols"

 
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         <ul id="sidebar" class="nav nav-stacked">
 
         <ul id="sidebar" class="nav nav-stacked">
 
             <li>
 
             <li>
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                 <a href="#GroupA">Background</a>
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                 <a href="#introduction">Protocols</a>
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            </li>
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                  <ul class="nav nav-stacked">
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                    <li>
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                <a href="#GroupB">Project</a>
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                        <a href="#media">Media and Solutions</a>
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                    </li>
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                    <li><a href="#BioBricks">BioBricks</a></li>
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                    <li>
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                    <li><a href="#Prediction">Prediction</a></li>
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                        <a href="#dnamanipulation">DNA Manipulation</a>
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                </ul>
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                    </li>
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            </li>
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                    <li>
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                        <a href="#transformations">Transformations</a>
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                    </li>
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                      <li>
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                          <a href="#other">Other</a>
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                    </li>
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                </ul>
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<div id="introduction" class="anchor"></div>
 
<div id="introduction" class="anchor"></div>
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<h1>Protocols</h1>
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<!--<h1>Protocols</h1>-->
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<p>Given the differing levels of experience in the team, and the plethora of protocols available on the net we decided to standardise our protocols and keep them in a tidy little orange folder. Here are most of the protocols we used over the year including our standard template.</p>  
  
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<img src="https://static.igem.org/mediawiki/2015/7/73/BABS_UNSW_Australia_protocols_example2.png" style="max-width:200%;max-height:200%;PADDING-LEFT: 50px;PADDING-RIGHT:50px; PADDING-TOP:50px; height:300px;float:right;clear:right;">
  
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<li><a href="https://static.igem.org/mediawiki/2015/1/18/BABS_UNSW_Australia_protocols_1MNa2CO3.pdf">1M Na2CO3</a></li>
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<div id="template" class="anchor"></div>
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<p><strong>Template</strong></p>
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<li><a href="https://static.igem.org/mediawiki/2015/c/c4/BABS_UNSW_Australia_protocols_stdunswbabsigem.pdf">Babs UNSW iGEM protocol template</a></li>
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<div id="media" class="anchor"></div>
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<p><strong>Media and Solutions</strong>
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<li><a href="https://static.igem.org/mediawiki/2015/4/4f/BABS_UNSW_Australia_protocols_LBbrothprep.pdf">LB Broth Preparation</a></li>
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<li><a href="https://static.igem.org/mediawiki/2015/d/da/BABS_UNSW_Australia_protocols_LB_agar_preparation.pdf">LB Agar Preparation</a></li>
 
<li><a href="https://static.igem.org/mediawiki/2015/1/1f/BABS_UNSW_Australia_protocols_Agarplateprep.pdf">Agar Plate Preparation</a></li>
 
<li><a href="https://static.igem.org/mediawiki/2015/1/1f/BABS_UNSW_Australia_protocols_Agarplateprep.pdf">Agar Plate Preparation</a></li>
 
<li><a href="https://static.igem.org/mediawiki/2015/f/fb/BABS_UNSW_Australia_protocols_AntibioticPrep.pdf">Antibiotic Preparation</a></li>
 
<li><a href="https://static.igem.org/mediawiki/2015/f/fb/BABS_UNSW_Australia_protocols_AntibioticPrep.pdf">Antibiotic Preparation</a></li>
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<li><a href="https://static.igem.org/mediawiki/2015/2/23/BABS_UNSW_Australia_protocols_Ecolitransformation.pdf">E.coli Transformation</a></li>
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<li><a href="https://static.igem.org/mediawiki/2015/a/ad/BABS_UNSW_Australia_protocols_strepregenmedia.pdf">Streptococcal Regeneration Media</a></li>
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<li><a href="https://static.igem.org/mediawiki/2015/8/85/BABS_UNSW_Australia_protocols_GM17mediaprep.pdf">GM17 Media Preparation</a></li>
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<li><a href="https://static.igem.org/mediawiki/2015/a/a2/BABS_UNSW_Australia_protocols_SOCprepiGEM.pdf">SOC Media Preparation</a></li>
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<li><a href="https://static.igem.org/mediawiki/2015/e/e2/BABS_UNSW_Australia_protocols_trisprep.pdf">Preparation of Tris-HCL Buffer</a></li>
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<li><a href="https://static.igem.org/mediawiki/2015/1/18/BABS_UNSW_Australia_protocols_1MNa2CO3.pdf">1M Na2CO3</a></li>
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</p>
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<div id="dnamanipulation" class="anchor"></div>
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<p><strong>DNA Manipulation</strong>
 
<li><a href="https://static.igem.org/mediawiki/2015/8/80/BABS_UNSW_Australia_protocols_EnzymeDigestion.pdf">Enzyme Digestion</a></li>
 
<li><a href="https://static.igem.org/mediawiki/2015/8/80/BABS_UNSW_Australia_protocols_EnzymeDigestion.pdf">Enzyme Digestion</a></li>
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<li><a href="https://static.igem.org/mediawiki/2015/7/7e/BABS_UNSW_Australia_protocols_Ligationigem.pdf">Ligation iGEM protocol</a></li>
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<li><a href="https://static.igem.org/mediawiki/2015/1/17/BABS_UNSW_Australia_protocols_V2ligationiGEM.pdf">Ligation iGEM protocol V2</a></li>
 
<li><a href="https://static.igem.org/mediawiki/2015/5/5a/BABS_UNSW_Australia_protocols_Gelelectrophoresis.pdf">Gel Electrophoresis</a></li>
 
<li><a href="https://static.igem.org/mediawiki/2015/5/5a/BABS_UNSW_Australia_protocols_Gelelectrophoresis.pdf">Gel Electrophoresis</a></li>
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<li><a href="https://static.igem.org/mediawiki/2015/8/85/BABS_UNSW_Australia_protocols_GM17mediaprep.pdf">GM17 Media Preparation</a></li>
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</p>
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<li><a href="https://static.igem.org/mediawiki/2015/1/1f/BABS_UNSW_Australia_protocols_Agarplateprep.pdf">Agar Plate Preparation</a></li>
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<li><a href="https://static.igem.org/mediawiki/2015/1/1f/BABS_UNSW_Australia_protocols_Agarplateprep.pdf">Agar Plate Preparation</a></li>
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<li><a href="https://static.igem.org/mediawiki/2015/1/1f/BABS_UNSW_Australia_protocols_Agarplateprep.pdf">Agar Plate Preparation</a></li>
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<li><a href="https://static.igem.org/mediawiki/2015/1/1f/BABS_UNSW_Australia_protocols_Agarplateprep.pdf">Agar Plate Preparation</a></li>
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<li><a href="https://static.igem.org/mediawiki/2015/1/1f/BABS_UNSW_Australia_protocols_Agarplateprep.pdf">Agar Plate Preparation</a></li>
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<li><a href="https://static.igem.org/mediawiki/2015/1/1f/BABS_UNSW_Australia_protocols_Agarplateprep.pdf">Agar Plate Preparation</a></li>
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<li><a href="https://static.igem.org/mediawiki/2015/1/1f/BABS_UNSW_Australia_protocols_Agarplateprep.pdf">Agar Plate Preparation</a></li>
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<div id="transformations" class="anchor"></div>
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<p><strong>Transformations</strong>
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<li><a href="https://static.igem.org/mediawiki/2015/2/23/BABS_UNSW_Australia_protocols_Ecolitransformation.pdf">E.coli Transformation</a></li>
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<li><a href="https://static.igem.org/mediawiki/2015/5/54/BABS_UNSW_Australia_protocols_prepoflactocompcells.pdf">Preparation of Lactococcus Competent Cells</a></li>
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<li><a href="https://static.igem.org/mediawiki/2015/1/1f/BABS_UNSW_Australia_protocols_lactopretransprepsol.pdf">Lactococcus electrotransformation pre-treatement solution</a></li>
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<li><a href="https://static.igem.org/mediawiki/2015/2/21/BABS_UNSW_Australia_protocols_simplelactotrans.pdf">Simple Lactococcus transformation</a></li>
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<li><a href="https://static.igem.org/mediawiki/2015/b/bc/BABS_UNSW_Australia_protocols_lactotransV1.pdf">Lactococcus Transformation V1</a></li>
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<li><a href="https://static.igem.org/mediawiki/2015/7/7b/BABS_UNSW_Australia_protocols_lactotransV2.pdf">Lactococcus Transformation V2</a></li>
 +
</p>
  
 +
<div id="other" class="anchor"></div>
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<p><strong>Other</strong>
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<li><a href="https://static.igem.org/mediawiki/2015/d/dc/BABS_UNSW_Australia_protocols_MiniprepiGEM.pdf">Miniprep iGEM protocol</a></li>
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<li><a href="https://static.igem.org/mediawiki/2015/4/42/BABS_UNSW_Australia_protocols_PCRpurificationiGEM.pdf">PCR purification iGEM protocol</a></li>
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<li><a href="https://static.igem.org/mediawiki/2015/9/92/BABS_UNSW_Australia_protocols_OverlapPCR.pdf">Overlap PCR</a></li>
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<li><a href="https://static.igem.org/mediawiki/2015/7/7c/BABS_UNSW_Australia_protocols_sequenceprepigem.pdf">Sequencing preparation iGEM Protocol</a></li>
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<li><a href="https://static.igem.org/mediawiki/2015/0/00/BABS_UNSW_Australia_protocols_mammaliancellassay.pdf">Mammalian Cell assay</a></li>
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<li><a href="https://static.igem.org/mediawiki/2015/b/b5/Unsw2015lacz.pdf">LacZ Assay</a></li>
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<li><a href="https://static.igem.org/mediawiki/2015/d/de/Unsw2015sdspage.pdf">SDS-PAGE protein gel</a></li>
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Latest revision as of 13:28, 18 September 2015

Given the differing levels of experience in the team, and the plethora of protocols available on the net we decided to standardise our protocols and keep them in a tidy little orange folder. Here are most of the protocols we used over the year including our standard template.

Template

  • Babs UNSW iGEM protocol template

    • Media and Solutions

  • LB Broth Preparation
  • LB Agar Preparation
  • Agar Plate Preparation
  • Antibiotic Preparation
  • Streptococcal Regeneration Media
  • GM17 Media Preparation
  • SOC Media Preparation
  • Preparation of Tris-HCL Buffer
  • 1M Na2CO3

    • DNA Manipulation

  • Enzyme Digestion
  • Ligation iGEM protocol
  • Ligation iGEM protocol V2
  • Gel Electrophoresis

    • Transformations

  • E.coli Transformation
  • Preparation of Lactococcus Competent Cells
  • Lactococcus electrotransformation pre-treatement solution
  • Simple Lactococcus transformation
  • Lactococcus Transformation V1
  • Lactococcus Transformation V2

    • Other

  • Miniprep iGEM protocol
  • PCR purification iGEM protocol
  • Overlap PCR
  • Sequencing preparation iGEM Protocol
  • Mammalian Cell assay
  • LacZ Assay
  • SDS-PAGE protein gel