Difference between revisions of "Team:NYU Shanghai/DailyNotes"
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<td><a href="#june12">12</a></td> | <td><a href="#june12">12</a></td> | ||
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| − | <td>14</td><td>15</td><td>16</td><td>17</td><td>18</td><td>19</td><td>20</td> | + | <td>14</td> |
| + | <td><a href=#june15>15</a></td> | ||
| + | <td><a href=#june16>16</a></td> | ||
| + | <td><a href=#june17>17</a></td> | ||
| + | <td><a href=#june18>18</a></td> | ||
| + | <td><a href=#june19>19</a></td> | ||
| + | <td>20</td> | ||
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| − | <td>21</td><td>22</td><td>23</td><td>24</td><td>25</td><td>26</td><td>27</td> | + | <td>21</td> |
| + | <td><a href=#june22>22</a></td> | ||
| + | <td><a href=#june23>23</a></td> | ||
| + | <td><a href=#june24>24</a></td> | ||
| + | <td><a href=#june25>25</a></td> | ||
| + | <td><a href=#june26>26</a></td> | ||
| + | <td>27</td> | ||
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| − | <td>28</td><td>29</td><td>30</td><td> </td><td> </td><td> </td><td> </td> | + | <td>28</td> |
| + | <td><a href=#june29>29</a></td> | ||
| + | <td><a href=#june30>30</a></td> | ||
| + | <td> </td><td> </td><td> </td><td> </td> | ||
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| − | <td> | + | <td> . </td><td> . </td><td> . </td><td> . </td><td> . </td><td> . </td><td> . </td> |
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| + | <td><a href=#July1>1</a></td> | ||
| + | <td><a href=#July2>2</a></td> | ||
| + | <td><a href=#July3>3</a></td> | ||
| + | <td> 4</td> | ||
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| + | <td><a href=#July6>6</a></td> | ||
| + | <td><a href=#July7>7</a></td> | ||
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| + | <td><a href=#July9>9</a></td> | ||
| + | <td><a href=#July10>10</a></td> | ||
| + | <td>11</td> | ||
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| + | <td><a href=#July13>13</a></td> | ||
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| + | <td>18</td> | ||
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| + | <td><a href=#July20-24>20</a></td> | ||
| + | <td><a href=#July20-24>21</a></td> | ||
| + | <td><a href=#July20-24>22</a></td> | ||
| + | <td><a href=#July20-24>23</a></td> | ||
| + | <td><a href=#July20-24>24</a></td> | ||
| + | <td>25</td> | ||
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| − | <td>26</td><td>27</td><td>28</td><td>29</td><td>30</td><td>31</td><td> | + | <td>26</td> |
| + | <td><a href=#July27>27</a></td> | ||
| + | <td><a href=#July28>28</a></td> | ||
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| + | <td><a href=#July30>30</a></td> | ||
| + | <td><a href=#July31>31</a></td> | ||
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| − | <td> | + | <td>.</td><td> . </td><td> . </td><td> . </td><td> . </td><td> . </td><td> . </td> |
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<td> </td><td> </td><td> </td><td> </td><td> </td><td> </td><td> 1</td> | <td> </td><td> </td><td> </td><td> </td><td> </td><td> </td><td> 1</td> | ||
</tr><tr> | </tr><tr> | ||
| − | <td> 2</td><td> 3</td><td> 4</td><td> 5</td><td> 6</td><td> 7</td><td> 8</td> | + | <td> 2</td> |
| + | <td><a href=#Aug3>3</a></td> | ||
| + | <td><a href=#Aug4>4</a></td> | ||
| + | <td><a href=#Aug5>5</a></td> | ||
| + | <td><a href=#Aug6>6</a></td> | ||
| + | <td><a href=#Aug7>7</a></td> | ||
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| − | <td> 9</td><td>10</td><td>11</td><td>12</td><td>13</td><td>14</td><td>15</td> | + | <td> 9</td> |
| + | <td><a href=#Aug10>10</a></td> | ||
| + | <td><a href=#Aug11>11</a></td> | ||
| + | <td><a href=#Aug12>12</a></td> | ||
| + | <td><a href=#Aug13-15>13</a></td> | ||
| + | <td><a href=#Aug13-15>14</a></td> | ||
| + | <td><a href=#Aug13-15>15</a></td> | ||
</tr><tr> | </tr><tr> | ||
| − | <td>16</td><td>17</td><td>18</td><td>19</td><td>20</td><td>21</td><td>22</td> | + | <td>16</td> |
| + | <td><a href=#Aug17>17</a></td> | ||
| + | <td><a href=#Aug18>18</a></td> | ||
| + | <td><a href=#Aug19>19</a></td> | ||
| + | <td><a href=#Aug20>20</a></td> | ||
| + | <td>21</td><td>22</td> | ||
</tr><tr> | </tr><tr> | ||
<td>23</td><td>24</td><td>25</td><td>26</td><td>27</td><td>28</td><td>29</td> | <td>23</td><td>24</td><td>25</td><td>26</td><td>27</td><td>28</td><td>29</td> | ||
</tr><tr> | </tr><tr> | ||
| − | <td>30</td><td>31</td><td> | + | <td>30</td><td>31</td><td> . </td><td> . </td><td> . </td><td> . </td><td> . </td> |
</tr> | </tr> | ||
</table> | </table> | ||
</div> | </div> | ||
</div> | </div> | ||
| − | <div style="width: 100%; height: | + | <div style="width: 100%; height: 150px;"></div> |
| + | <div id="pdfs"> | ||
| + | <span class="scrollable"></span> | ||
| + | <br> | ||
| + | <h6><font color="#d66">Lab Journals</font></h6> | ||
| + | <br><p>Click a name to link to a pdf of their lab journal.</p> | ||
| + | <p> <a href="https://static.igem.org/mediawiki/2015/0/01/NYU_Shanghai_Misyanotebook.pdf"><span>Misya</a></span> | ||
| + | <br><a href="https://static.igem.org/mediawiki/2015/4/49/NYU_Shanghai_Spencernotebook.pdf"><span>Spencer</a></span> | ||
| + | <br><a href="https://static.igem.org/mediawiki/2015/8/86/NYU_Shanghai_Reidanotebook.pdf"><span>Reida</a></span> | ||
| + | <br><a href="https://static.igem.org/mediawiki/2015/c/ce/NYU_Shanghai_Annnotebook.pdf"><span>Ann</a></span> | ||
| + | <br><a href="https://static.igem.org/mediawiki/2015/c/c9/NYU_Shanghai_Rachelnotebook.pdf"><span>Rachel</a></span> | ||
| + | </p> | ||
| + | </div> | ||
| + | <div id="pdfs"> | ||
| + | <h6><font color="#d66">Summary Timeline</font></h6> | ||
| + | <br><br> | ||
| + | <table border="1"> | ||
| + | <tr> | ||
| + | <td>September</td> | ||
| + | <td>Initial discussions with administrators and instructors about starting an iGEM team</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>October</td> | ||
| + | <td>Initial discussions with student body</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>November</td> | ||
| + | <td>Student team forming. Skype meetings with previous iGEM teams</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>December</td> | ||
| + | <td>Time spent learning about synthetic biology, plasmids, and laboratory concepts</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>January</td> | ||
| + | <td>Time spent learning about synthetic biology, plasmids, and laboratory concepts</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Febuary</td> | ||
| + | <td>Initial discussions about iGEM project. Instructors on board with advising.</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>March</td> | ||
| + | <td>First iGEM project no longer seems not feasible. Discussions about a second idea. iGEM officially sponsored by our school.</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>April</td> | ||
| + | <td>A full team officially committed to iGEM</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>May</td> | ||
| + | <td>Preparing for the summer, and finals</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>June</td> | ||
| + | <td>We step into the lab. We are still finalizing our project idea</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>July</td> | ||
| + | <td>Into the daily grind</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>August</td> | ||
| + | <td>Rushing to finish</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>September</td> | ||
| + | <td>Preparing for Boston: wiki, poster, presentation, final touches on the project</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | </div> | ||
<div id="timeline"> | <div id="timeline"> | ||
| − | < | + | <h6><font color="#d66">Daily Notes</font></h6> |
| − | + | ||
<h3><span id="may25"></span>25 May 2015</h3> | <h3><span id="may25"></span>25 May 2015</h3> | ||
| − | + | <p>Arduino Workshop 001(What is Arduino? How to blink a built-in LED and how to use a button to control the LED), Interactive Media Arts Lab Tour, Biology Lab Safety Training, Biology Lab Tour</p> | |
<h3><span id="may26"></span>26 May 2015</h3> | <h3><span id="may26"></span>26 May 2015</h3> | ||
| − | + | <p>Skype meeting with Tristan, who is currently in the States, about our wiki page and final project prototype. Discussions on how to make editing the wiki accessible to everyone, the content of workshops, fundraising, and the coming conference at NCTU. Research on luciferase enzyme, oscillations, and rfc10.</p> | |
<h3><span id="may27"></span>27 May 2015</h3> | <h3><span id="may27"></span>27 May 2015</h3> | ||
| − | + | <p>Bioluminescence presentation given by Ann. Arduino with color sensor+RGB led testing. Research about oscillating systems from previous competitions. Specifics regarding the plasmids we will be implementing. Successfully booked four flights to Taiwan for NCTU Conference in July.</p> | |
<h3><span id="may28"></span>28 May 2015</h3> | <h3><span id="may28"></span>28 May 2015</h3> | ||
| − | + | <p>Skype testing with Tristan for tomorrow’s HTML workshop. Talking with Fudan University about setting up a workshop. Overall unproductive.</p> | |
<h3><span id="may29"></span>29 May 2015</h3> | <h3><span id="may29"></span>29 May 2015</h3> | ||
| − | + | <p>Tristan’s Workshop on HTML via Google Hangout, Reida’s Workshop on plasmids construction, June’s Workshop on penicillin. We also got meal vouchers provided by NYU Shanghai! Hurray!</p> | |
<h3><span id="may31"></span>31 May 2015</h3> | <h3><span id="may31"></span>31 May 2015</h3> | ||
| − | + | <p>We went to EXPLORIUM to hold a workshop with kids and taught them how to visualize their DNA collected from their mouth with detergent, salt, starch, and alcohol.</p> | |
<h3><span id="june01"></span>1 June 2015</h3> | <h3><span id="june01"></span>1 June 2015</h3> | ||
| − | + | <p>Misya’s presentation on E.chromi. IDT representatives visited our lab and showed us how to order DNA on the website. Introduction to transformation lab with arabinose, pGLO, nutrient broth, and ampicillin. Bio majors practiced lab skills and taught Interactive Media Arts majors how to do lab. It was fun! Let’s see if our E.coli can glow tomorrow!.</p> | |
<h3><span id="june02"></span>2 June 2015</h3> | <h3><span id="june02"></span>2 June 2015</h3> | ||
| − | + | <p>Spencer’s presentation on biological oscillators. The results of yesterday’s lab were kind of disappointing. GFP was not expressed and not visible under UV light. Most likely because of old agar plates. Bio majors tested our first transformation using a part from the kit (RFP generator) and made new agar plates. Bruno helped ZZ build our new color-tracking system with a web camera and Processing.</p> | |
<h3><span id="june03"></span>3 June 2015</h3> | <h3><span id="june03"></span>3 June 2015</h3> | ||
| − | + | <p>Bruno, Christian, and Xiaoyue’s workshop on Virtual Reality of the inside of cell with the help of Unity 3D. Got chance to experience the bacteria world with the brand-new and exciting tool. Also, Reida’s RFP plasmid transformation showed a fluorescence in one colony! Prep work for second transformation lab including CFP, RFP, and GFP.</p> | |
<h3><span id="june04"></span>4 June 2015</h3> | <h3><span id="june04"></span>4 June 2015</h3> | ||
| − | + | <p>Xiangxi’s presentation on mathematical modeling. Tristan’s Google Hangout workshop on Java/Animation in HTML. Bio majors made 54 LB agar plates with different concentrations of chloramphenicol resistances (12.5, 25, 34, 50 mg/ml).</p> | |
<h3><span id="june05"></span>5 June 2015</h3> | <h3><span id="june05"></span>5 June 2015</h3> | ||
| − | + | <p>Ann’s presentation on Mini-prep. Everyone did Mini-Prep lab. DAN Quantitation of Mini-Prep we did. Xiangci talked about developing Virtual Reality Project for Human Practice with Christian. Photo shoot for our wiki landing page!</p> | |
<h3><span id="june08"></span>8 June 2015</h3> | <h3><span id="june08"></span>8 June 2015</h3> | ||
| − | + | <p>Research about oscillation sequences. Meet up with Tongji (TJU), Jiaotong (SJTU), IDP students (Indonesia)</p> | |
<h3><span id="june09"></span>9 June 2015</h3> | <h3><span id="june09"></span>9 June 2015</h3> | ||
| − | + | <p>Reida’s presentation on Gel Electrophoresis and Gel Extraction. Lab prep for tomorrow’s Gel Extraction lab. Building up website. Analysis of the medal criteria.</p> | |
<h3><span id="june10"></span>10 June 2015</h3> | <h3><span id="june10"></span>10 June 2015</h3> | ||
| − | + | <p>Human Practice | Bioluminescence + Arduino Workshop in Kongjiang High School<br>Misya’s presentation on competent cells. Gel Electrophoresis Lab. Workshop about Bioluminescence, Arduino, and the integration of technology and biology in Kongjiang University as one of the series workshops that we are going to have in schools as human practice projects.</p> | |
<h3><span id="june11"></span>11 June 2015</h3> | <h3><span id="june11"></span>11 June 2015</h3> | ||
<p>Spencer’s presentation on Art & Design track. Brainstormed more details of our project. pGLO glowed! Bio majors transformed luciferase generator parts from the kit. </p> | <p>Spencer’s presentation on Art & Design track. Brainstormed more details of our project. pGLO glowed! Bio majors transformed luciferase generator parts from the kit. </p> | ||
<h3><span id="june12"></span>12 June 2015</h3> | <h3><span id="june12"></span>12 June 2015</h3> | ||
| − | + | <p>Ann led a recap session before her 2 week leave. Lab team made competent cells. Strikingly page is up for sponsors!</p> | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
<h3><span id="june15"></span>15 June 2015</h3> | <h3><span id="june15"></span>15 June 2015</h3> | ||
| − | + | <p>Started working on Virtual Reality (VR). Made a lot of agar plates and transformed chromoprotein parts.</p> | |
<h3><span id="june16"></span>16 June 2015</h3> | <h3><span id="june16"></span>16 June 2015</h3> | ||
| − | + | <p>RFP glows! Inoculation of chromprotein parts.</p> | |
| − | <h3><span id=" | + | <h3><span id="june17"></span>17 June 2015</h3> |
| − | + | <p>Miniprep of chromoprotein parts. Nanodrop results could have been better...our average was 18.8 ng/uL. We just need a little more practice? Our IMA tech guy introduced some new ideas for our final project, and we discussed with him new ways of integrating the colorful colonies into a step sequencer.</p> | |
| − | <h3><span id=" | + | <h3><span id="june18"></span>18 June 2015</h3> |
| − | + | <p>Spencer's workshop on plasmid construction. Tried to do restriction digest, but no results seen on the gel (ES). We tried following NEB protocol (not iGEM) and it also didn't show on the gel.</p> | |
| − | <h3><span id=" | + | <h3><span id="june19"></span>19 June 2015</h3> |
| − | + | <p>Skype meeting with ZZ to discuss more details of final project. Another round of restriction digest (Xbal and PstI), and it worked!</p> | |
| − | <h3><span id=" | + | <h3><span id="june23"></span>23 June 2015</h3> |
| − | + | <p>More development on the design of the final project. Attempt sequential digest with EcoRI and SpeI. No results. </p> | |
| − | <h3><span id=" | + | <h3><span id="june24"></span>24 June 2015</h3> |
| − | + | <p>Skype meeting with one of our potential sponsors. Developed design of Processing part. Another digest, this time a double digest with EcoRI and SpeI. Digest successful! </p> | |
| − | <h3><span id=" | + | <h3><span id="june25"></span>25 June 2015</h3> |
| − | + | <p>Budget and sponsorship meeting with NYU Shanghai Finance Office and Foundation. Skyped with Vivan Xu to brainstorm our sponsorship/project. </p> | |
| − | <h3><span id=" | + | <h3><span id="june26"></span>26 June 2015</h3> |
| − | + | <p>Getting our member Rachel on board! Development of our interface. </p> | |
| − | <h3><span id=" | + | <h3><span id="june26"></span>26 June 2015</h3> |
| − | + | <p>Coding day for ZZ and Xiangci. Pictures of plates can play notes now. First transformation of ligation products. Sohigh still working on Virtual Reality. </p> | |
| − | <h3><span id=" | + | <h3><span id="june29"></span>29 June 2015</h3> |
| − | + | <p>Coding day for ZZ and Xiangci. Pictures of plates can play notes now. First transformation of ligation products. Sohigh still working on Virtual Reality. </p> | |
| − | <h3><span id=" | + | <h3><span id="june30"></span>30 June 2015</h3> |
| − | + | <p>Lab work presentations with professors. Processing code development. More talks with potential sponsors. Plates photo booth set up. </p> | |
| − | <h3><span id=" | + | <h3><span id="July1"></span>1 July 2015</h3> |
| − | + | <p>Coding coding coding coding. Testing music components. Obtaining linearized backbone through gel electrophoresis and gel extraction. </p> | |
| − | <h3><span id=" | + | <h3><span id="July2"></span>2 July 2015</h3> |
| − | + | <p>Google cardboards arrived! More progress on sponsorship and details of our final interactive genetically inspired music production installation. </p> | |
| − | <h3><span id=" | + | <h3><span id="July3"></span>3 July 2015</h3> |
| − | + | <p>Laser cut a tube rack sample. Lab team did more work on digests and ligations. VR team built a 3D plasmid model. Two official donation agreements sent out. Starting initial wiki design. </p> | |
| − | <h3><span id=" | + | <h3><span id="July6"></span>6 July 2015</h3> |
| − | + | <p>Presentation for the professors on all the stuff besides Biology. Project interface designed in Illustrator. VR team built a skybox in Unity. Made a tube rack prototype. </p> | |
| − | <h3><span id=" | + | <h3><span id="July7"></span>7 July 2015</h3> |
| − | + | <p>Preparation for the iGEM Conference in NCTU. Settled down many cool Arduino stuff that we are going to get from DFRobot. VR tested our Unity project on an Android phone. An academic debate in bio lab over troubleshooting procedures for an unsuccessful ligation. New digestion of promoter and blue chromoprotein with lower reaction volumes. </p> | |
| − | <h3><span id=" | + | <h3><span id="July8"></span>8 July 2015</h3> |
| − | + | <p>Taiwan planning. Tube rack processing code. VR team successfully ran our Unity file on an Android phone! The full construct with Promoter-RBS-Lime green chromoprotein-Terminator grew! But no color seen... </p> | |
| − | <h3><span id=" | + | <h3><span id="July9"></span>9 July 2015</h3> |
| − | + | <p>Planning for Taiwan Conference trip is done! More sponsorship letters sent out. </p> | |
| − | <h3><span id=" | + | <h3><span id="July10"></span>10 July 2015</h3> |
| − | + | <p>No colonies for RFP, YFP, CFP, GFP parts. Why? Luciferase parts successfully transformed into HB101 but still no color seen. Animation work for NCTU Conference begins. </p> | |
| − | <h3><span id=" | + | <h3><span id="July13"></span>13 July 2015</h3> |
| − | + | <p>A lot of transformations. Tested the usb webcame modules. Interviewed bio-artist Vivian Xu, DJ Benjamin Bacon about biology, art, step sequencers, and experimental music. Our Strikingly page made it to the Strikingly discover page! http://igemnyushanghai.strikingly.com </p> | |
| − | <h3><span id=" | + | <h3><span id="July14"></span>14 July 2015</h3> |
| − | + | <p>FUN DAY! Filmed our swag team introduction video for the coming NCTU iGEM conference and took group photos. Got team T-shirts for the NCTU conference! Got a sponsorship package from DFRobot filled with Arduino boards, sliders, buttons, LEDs, and many other cool electronic components for our installation. DFRobot is awesome!</p> | |
| − | <h3><span id=" | + | <h3><span id="July15"></span>15 July 2015</h3> |
| − | + | <p>Miniprep inoculated cultures (promoter + rbs + chromoprotein constructs).Finished editing team video. Poster for NCTU near completion. </p> | |
| − | <h3><span id=" | + | <h3><span id="July16"></span>16 July 2015</h3> |
| − | + | <p>Digest, ligation, and transformation of promoter + rbs + chromprotein + terminator constructs. We should see color tomorrow? Others are animating, coding, making posters, presentations, and updating the budget.</p> | |
| − | <h3><span id=" | + | <h3><span id="July17"></span>17 July 2015</h3> |
| − | + | <p>Getting ready for the Asia conference. </p> | |
| − | <h3><span id=" | + | <h3><span id="July20-24"></span>20-24 July 2015</h3> |
| − | + | <p>Attended the iGEM Asia Conference hosted by National Chiao Tong University. We learned a lot from other teams and met some really awesome people! We also took a couple days off to explore Taipei area. A good trip. </p> | |
| − | <h3><span id=" | + | <h3><span id="July28"></span>28 July 2015</h3> |
| − | + | <p>Report of our Taiwan trip to our Professors. Potential troubleshooting methods for our lack of color included: adding a RFP reporter gene, sending the parts for seqeuencing, restarting construction from the beginning, and experimenting with arabinose concentrations. </p> | |
| − | <h3><span id=" | + | <h3><span id="July29"></span>29 July 2015</h3> |
| − | + | <p>Our IDT plasmids arrived! Realized only 1000ng of DNA came, thus began designing primers so that we could amplify the sequence (never designed primers before–Snapgene is a great tool!). Sangon agreed to be a donator! Rethinking about our physical installation. Thankful that NEB sent us free Q5 polymerase earlier in the summer.</p> | |
| − | <h3><span id=" | + | <h3><span id="July30"></span>30 July 2015</h3> |
| − | + | <p>Welcoming our new tech helper--Lewei (Richard) Huang! Also interviewed by The Good Life magazine! Figuring out the data transfer of our new installation. Sangon came to pick up our constructed plasmids for sequencing. Realized that iGEM primers are not common primers...we'll see how sequencing goes.</p> | |
| − | <h3><span id=" | + | <h3><span id="July31"></span>31 July 2015</h3> |
| − | + | <p>Went to Fudan University, had lunch with their team members, and learned about the sequencing process, using T-vecors, ordering primers, and general lab procedures. They also gave us their iGEM kits, since we ran out of luciferase generators (our last transformation didn't work). Thank you Fudan iGEM Team! </p> | |
| − | <h3><span id=" | + | <h3><span id="Aug3"></span>3 Aug 2015</h3> |
| − | + | <p>Consulted Antonius about our coding difficulties and he offered some good suggestions. Two cameras running with one Processing sketch! Yay! Spencer is back from America, back to making chromoprotein constructs.</p> | |
| − | <h3><span id=" | + | <h3><span id="Aug4"></span>4 Aug 2015</h3> |
| − | + | <p>Virtual Reality project still making progress. Xiangci still coding. Lab meeting held, and new lab policies established to make work more efficient (Friday is inventory check-day, pre-lab requirements, attendance time, etc.) </p> | |
| − | <h3><span id=" | + | <h3><span id="Aug5"></span>5 Aug 2015</h3> |
| − | + | <p>Getting ready for the 8 iGEM Teams project exhibition at the Shanghai Science and Technology Museum. Gel extraction of digested backbones (PCR is probably a much more efficent way to do this). </p> | |
| − | <h3><span id=" | + | <h3><span id="Aug6"></span>6 Aug 2015</h3> |
| − | + | <p>Code reconstructed and the we can produce live music now! Hurray! First PCR of the summer–amplified gBlocks recieved from IDT. Found out we needed to add luciferin + ATP to the luciferase generator to generate light.</p> | |
| − | <h3><span id=" | + | <h3><span id="Aug7"></span>7 Aug 2015</h3> |
| − | + | <p>PCR Purification came out imperfect (a 2.77 260:280 ratio?). Ran another PCR for less non-specific bands. Installation makers and coders hurry to prep for tomorrow.</p> | |
| − | <h3><span id=" | + | <h3><span id="Aug8"></span>8 Aug 2015</h3> |
| − | + | <p>iGEM Show at Shanghai Science and Technology Museum! Along with Fudan, Shanghai Jiaotong, Tongji Wetware, Tongji Software, Xian-Jiaotong Liverpool, and Zhejiang University. Our bacterial music generator and virtual reality simulations were a success!</p> | |
| − | <h3><span id=" | + | <h3><span id="Aug10"></span>10 Aug 2015</h3> |
| − | + | <p>Digested and ligated IDT sequences to backbone. If ligation went well, we should see color tomorrow...Sequencing results also back, and all of our previous constructs contained only GFP and CFP, not even a promoter. </p> | |
| − | <h3><span id=" | + | <h3><span id="Aug11"></span>11 Aug 2015</h3> |
| − | + | <p>No color. Realized that pBAD promoter is inhibited by glucose, and that we've been using SOC media (containing glucose) to do all our transformations. Lesson learned. Talked with Xian-Jiaotong Liverpool University as to how to produce color (they are also working with chromoproteins). Got our LED light strip for our plate stage. Did the test run on the laser cutter for the camera stand.</p> | |
| − | <h3><span id=" | + | <h3><span id="Aug12"></span>12 Aug 2015</h3> |
| − | + | <p>Ann, Sohigh, and Spencer left for the Beijing University iGEMers Conference! Rachel, ZZ, Xiangci, and June are still working hard in Shanghai. </p> | |
| − | <h3><span id=" | + | <h3><span id="Aug13-15"></span>13-15 Aug 2015</h3> |
| − | + | <p>The Beijing Conference was a great experience. We were definitely awe-struck by the level of research and development of the other teams. We also got to try to 'perform' our project--we turned of all the lights on stage before our presentation and it was a wonderfully dramatic experience. Thank you Beijing University! And thank you many other teams (like Shanghai Ocean University and Northeast Forestry University for their advice on working with the pBAD promoter and chromoproteins, respectively. </p> | |
| − | <h3><span id=" | + | <h3><span id="Aug17"></span>17 Aug 2015</h3> |
| − | + | <p>Last week of iGEM for us (before we all leave to different countries). Everything is a big rush now. </p> | |
| − | <h3><span id=" | + | <h3><span id="Aug18"></span>18 Aug 2015</h3> |
| − | + | <p>Today Xian-Jiaotong Liverpool University drove all the way from Suzhou (2 hours) to send us plasmids, bacteria containing plasmids, and extra bacteria! THANK YOU XJLTU! They were also working with chromoproteins, and sent us their materials so that we could produce some color in the lab before we left. </p> | |
| − | <h3><span id=" | + | <h3><span id="Aug19"></span>19 Aug 2015</h3> |
| − | + | <p>Another filming day, this time the final project is completely put together--step sequencer, tube rack, and button control. We planned to start shooting at 10AM, but a lot of difficulties kept coming up, so we ended up starting the shoot around 4pm. It was a really great feeling being able to see everything put together. Today was ZZ and Xiangci's last day.</p> | |
| − | <h3><span id=" | + | <h3><span id="Aug20"></span>20 Aug 2015</h3> |
| − | + | <p>The last day together. We cleaned out the lab fridge. It was very painful throwing away the summer's work of plasmids. We cleaned out the IMA lab and we our classroom 307. We made a round of thank you's to all the administration who helped set up iGEM. The work is still not done... we have the film editing, wiki, VR, T-shirts, presentations, posters, etc. But the next time we meet as a full team will be in Boston. So this is the last daily update of our iGEM journey.</p> | |
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Latest revision as of 15:49, 18 September 2015
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