Difference between revisions of "Team:BABS UNSW Australia/Parts"

Part Documentation

Lactoccocus Parts

BBa_K1677103: Invasin with LysM signal peptide (Lactococcus)
• This protein-coding part encodes invasin tailored for use in Lactococcus species.The signal peptide region of Listeria monocytogenes was exchanged for a signal peptide Lactobacillus plantarum. The aim of this is localise the invasin protein to the outer membrane when used in Lactococcus species. The LysM (lysine motif) protein domain embeds itself in the membrane, presenting the attached protein to the extracellular environment. The invasin gene was codon-optimised for expression in L. lactis. This part was assembled through Gibson assembly of three g-blocks designed with 20-30bp overlapping regions.
BBa_K1677101: Listeriolysin (Lactococcus)
• This part is listeriolysin codon-optimised for expression in Lactococcus. By co-expressing with the above LysM-INV, Lactococcus species can enter into mammalian cells. This part was assembled through overlap PCR of two g-blocks with a 20bp overlap.

Pseudoknots

BBa_K1677391: IBV Pseudoknot
• Sequence adapted from a pseudoknot sourced from the Infection Bronchitis Virus.
BBa_K1677369: MMTV Pseudoknot
• Sequence adapted from a pseudoknot sourced from the Mouse Mammary Tumor Virus.
BBa_K1677743: TGV Pseudoknot
• Sequence adapted from a pseudoknot sourced from the Transmissible Gastroenteritis Virus.
The above pseudoknots were submitted individually, for future use as translational regulatory mechanisms.

IPTG-inducible promoter + Pseudoknots

BBa_K1677800: IPTG inducible promoter with IBV pseudoknot BBa_K1677801: IPTG inducible promoter with TGV pseudoknot BBa_K1677802: IPTG inducible promoter with MMTV pseudoknot
• The above three parts

Parts Table

<groupparts>iGEM015 BABS_UNSW_Australia</groupparts>