Difference between revisions of "Team:NYU Shanghai/Protocols"
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<br><br><br> | <br><br><br> | ||
<h6><font color="#d66">Building our Construct: from IDT gBlocks</font></h6> | <h6><font color="#d66">Building our Construct: from IDT gBlocks</font></h6> | ||
+ | <p>Note: We recommend adding a reporter gene to the construct.</p> | ||
<br><img src="https://static.igem.org/mediawiki/2015/7/71/NYU_Shanghai_IDTprocedure.png" width="550"> | <br><img src="https://static.igem.org/mediawiki/2015/7/71/NYU_Shanghai_IDTprocedure.png" width="550"> | ||
<br><br><br> | <br><br><br> | ||
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<tr> | <tr> | ||
<td><font color="#d66">Initial Denaturation</font></td> | <td><font color="#d66">Initial Denaturation</font></td> | ||
− | <td | + | <td>98C</td> |
− | <td | + | <td>30s</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td><font color="#d66">25 cycles</td> | + | <td><font color="#d66">25 cycles</font></td> |
<td>98C</td> | <td>98C</td> | ||
<td>15s</td> | <td>15s</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>Final Extension</td> | + | <td><font color="#d66">Final Extension</font></td> |
<td>72C</td> | <td>72C</td> | ||
<td>2m</td> | <td>2m</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>Hold</td> | + | <td><font color="#d66">Hold</font></td> |
<td>4C</td> | <td>4C</td> | ||
<td></td> | <td></td> | ||
</tr> | </tr> | ||
+ | </table> | ||
</p> | </p> | ||
<p>Controls | <p>Controls |
Latest revision as of 16:56, 18 September 2015