Difference between revisions of "Team:Freiburg/Project/Overview"

 
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{{Freiburg/Menubar}}
 
{{Freiburg/Menubar}}
  
−
{{Freiburg/wiki_content_start}}
+
{{Team:Freiburg/wiki_content_start_bubble}}
 
<html>
 
<html>
−
<div class="content_box">
 
−
<!-- Labjournal content goes in here -->
 
−
 
  
−
<h1 class="sectionedit1">Project overview: The DiaCHIP</h1>
+
<style>
−
<div class="level1">
+
 
−
<div class="image_box left">
+
/*========= BEGIN: style for navigation bar ==========*/
−
<img align="left" alt="light bulb sketch" src="https://static.igem.org/mediawiki/2015/2/23/Freiburg_light_bulb_sketch.png" width="100px">
+
#project {
 +
    background: url(https://static.igem.org/mediawiki/2015/d/da/Freiburg_icon_project_active_yellow.png) no-repeat;
 +
}
 +
 
 +
#project a {
 +
    color: #ecdc18;
 +
}
 +
 
 +
#runningchip {
 +
    left: 18.6%;
 +
}
 +
/*========= END: style for navigation bar ==========*/
 +
 
 +
 
 +
/*========= BEGIN: Linkbutton in slider ============*/
 +
.intro_button a:hover {
 +
    text-decoration: underline;
 +
}
 +
 
 +
.intro_button a {
 +
    color: #FFF;
 +
}
 +
 
 +
.intro_button {
 +
    margin: 0px auto;
 +
    padding: 2px 5px 5px 10px;
 +
    width: 180px;
 +
    line-height: 22px;
 +
    text-align: center;
 +
    /*background-color: #326aab;*/
 +
    background-color: #0051A2;
 +
    font-size: 14pt;
 +
    border-radius: 8px;
 +
    /*box-shadow: 1px -1px 1px 0px #888;*/
 +
    /*border: 1px solid #F9F9F9;*/
 +
    color: #FFF;
 +
}
 +
 
 +
.menu-arrow{
 +
background-image: url("https://static.igem.org/mediawiki/2015/f/ff/Freiburg_submenu_arrow.png");
 +
background-repeat: no-repeat;
 +
background-position: 5% 50%;
 +
background-size: 10px 13px;
 +
}
 +
 
 +
/*========= END: Linkbutton in slider ============*/
 +
 
 +
.link_button {
 +
margin: 0px auto;
 +
padding: 0px 5px 0px 10px;
 +
width: 200px;
 +
line-height: 16px;
 +
text-align: center;
 +
background-color: #888;
 +
font-size: 14pt;
 +
border-radius: 2px;
 +
color: #FFF;
 +
opacity: 0.8;
 +
box-shadow: 1px 1px 1px 2px #777;
 +
}
 +
 
 +
.link_button a:hover {
 +
    text-decoration: underline;
 +
}
 +
 
 +
.link_button a {
 +
    color: #FFF;
 +
}
 +
 
 +
.link_button_arrow{
 +
background-image: url("https://static.igem.org/mediawiki/2015/f/ff/Freiburg_submenu_arrow.png");
 +
background-repeat: no-repeat;
 +
background-position: 3% 50%;
 +
background-size: 10px 13px;
 +
}
 +
 
 +
/*=========BEGIN: General Styles ==========*/
 +
.header_box h1{
 +
  color: #C5162F;
 +
  font-size: 40px;
 +
  margin-bottom: 28px;
 +
  margin-top: 20px;
 +
  line-height: 45px;
 +
  font-weight: 200;
 +
  padding-top: 0px;
 +
  padding-bottom: 0px;
 +
  overflow: visible;
 +
  text-align: center;
 +
}
 +
/*=========END: General Styles===========*/
 +
 
 +
 
 +
 
 +
@media screen and (min-width: 1000px){
 +
 
 +
/* code adapted from http://www.smashingmagazine.com/2012/04/pure-css3-cycling-slideshow/*/
 +
 
 +
#slider {
 +
  height: 500px;
 +
  width: 910px;
 +
  overflow: visible;
 +
  position: relative;
 +
}
 +
 
 +
/* hides everything outside the slider box */
 +
#mask {
 +
  overflow: hidden;
 +
  height: 500px;
 +
}
 +
 
 +
/* initialize position for positioning slides outside the slider */
 +
#slider ul {
 +
  margin: 0;
 +
  padding: 0;
 +
  position: relative;
 +
}
 +
 
 +
/* define image properties and position them outside the slider mask */
 +
#slider li {
 +
  width: 900px;  /* Width slide */
 +
  height: 500px; /* Height slide */
 +
  position: absolute;
 +
  right: 0; /* Original Position - Outside of the Slider */
 +
  list-style: none;
 +
}
 +
 
 +
 
 +
#first,
 +
#second,
 +
#third,
 +
#fourth,
 +
#fifth,
 +
#sixth,
 +
#seventh,
 +
#eightth {
 +
  left: -1000px;
 +
  -ms-transition: tranform 1s ease;
 +
  -webkit-transition: transform 1s ease;
 +
  transition: transform 1s ease;
 +
  font-size: 100%;
 +
}
 +
 
 +
#first{left: 0;}
 +
#third{z-index : -100;}
 +
#sixth{z-index: -100;}
 +
#fifth{z-index: -100;}
 +
#fourth{z-index: -100}
 +
#seventh{z-index: -100;}
 +
#eightth{z-index: -100; left: 1000px}
 +
 
 +
.container{
 +
    position: relative;
 +
    height: 500px;
 +
    margin: 3% auto;
 +
    width: 1000px;
 +
}
 +
 
 +
#buttonleft {
 +
  z-index: 200;
 +
  position: absolute;
 +
  top: 190px;
 +
  left: 0;
 +
}
 +
 
 +
#buttonright{
 +
  z-index: 200;
 +
  position: absolute;
 +
  right: 0;
 +
  top: 190px;
 +
}
 +
 
 +
#buttonleft img,
 +
#buttonright img{
 +
  width: 50px;
 +
}
 +
 
 +
#content-slider{
 +
  position: absolute;
 +
  top:0;
 +
  left:50px
 +
}
 +
 
 +
 
 +
#buttonright:hover,
 +
#buttonleft:hover{
 +
  cursor: pointer;
 +
  cursor: hand;
 +
}
 +
 
 +
.artboard{
 +
    box-sizing: border-box;
 +
    height: 470px;
 +
    border-radius: 60px;
 +
    background-color: #FCFCFC;
 +
  box-shadow: 1px 1px 10px rgb(136, 136, 136);
 +
  margin: 5px;
 +
  border: 2px solid rgb(0, 81, 162);
 +
}
 +
 
 +
.slidertext p{
 +
  margin-bottom: 0;
 +
}
 +
 
 +
.slidertext h1 {
 +
    text-align: center;
 +
}
 +
 
 +
.slidertext.indent{
 +
  clear: both;
 +
  margin-left: 15%;
 +
  margin-top: 0;
 +
  width: 290px;
 +
}
 +
 
 +
.dummy-image{
 +
  background-color: black;
 +
  height: 400px;
 +
  width: 350px;
 +
}
 +
 
 +
/* ============= BEGIN: Styling for tree-like structure =============== */
 +
 
 +
.floatcontainer{
 +
  float: left;
 +
}
 +
 
 +
.container{
 +
  clear: both;
 +
}
 +
 
 +
.transparent_container{
 +
  overflow: hidden
 +
}
 +
 
 +
#content_box1{
 +
  margin-left: 15%;
 +
}
 +
 
 +
#content_box2{
 +
  margin-right: 15%
 +
}
 +
 
 +
#content_box3{
 +
  margin-left: 15%;
 +
}
 +
 
 +
#leftimage img{
 +
  width: 450px;
 +
}
 +
 
 +
} /* end of media query */
 +
 
 +
.sliderimage{
 +
  float: right;
 +
  margin: 3% 70px 3% 25px;
 +
  width: 350px
 +
}
 +
 
 +
.slidertext{
 +
  margin: 3% 25px 0 65px;
 +
  width: 350px;
 +
  text-align: justify;
 +
  line-height: 1;
 +
  float: left;
 +
}
 +
 
 +
 
 +
/* ============= END: Styling for tree-like structure =============== */
 +
 
 +
</style>
 +
 
 +
<script type="text/javascript">
 +
//===================BEGIN:Amazing Bubble Sidebar==========================
 +
 
 +
$(document).ready(function(){
 +
  // CHANGE THE FOLLOWING ATTRIBUTES //
 +
  var href_text1='https://2015.igem.org/Team:Freiburg/Project/Overview',
 +
  // Text2 needs no href as it is the actual page //
 +
  img_url='https://static.igem.org/mediawiki/2015/7/76/Freiburg_icon_project_white_03.png',
 +
  href_text3='https://2015.igem.org/Team:Freiburg/Project/System',
 +
  // Text1 needs no text as it is a pic //
 +
  text2='Project Introduction';
 +
  // Text3 needs no text as its always 'next' //
 +
  // HOLD ON CHANGING THINGS --JABBERWOCK  //
 +
 
 +
  $('#bubble1').attr('href',href_text1);
 +
  $('#bubble1_img').attr('src', img_url);
 +
  $('#bubble3').attr('href',href_text3);
 +
 
 +
  $('#bubble2').text(text2);
 +
});
 +
 
 +
//===================END:Amazing Bubble Sidebar==========================
 +
</script>
 +
 
 +
<script>
 +
$(document).ready(function(){
 +
 
 +
    var distance = 2;
 +
    // see: http://atomicrobotdesign.com/blog/web-development/how-to-disable-a-button-until-a-jquery-animation-has-finished/ //
 +
    // bind the button to the function, so it can be unbound in the code and the button isnt clickable any more //
 +
    $("#buttonleft").bind("click", moveleft);
 +
  function moveleft(e) {
 +
    // unbind the button //
 +
    $("#buttonleft").unbind();
 +
    console.log("Click "+distance);
 +
 
 +
    if (distance == 1) {
 +
      console.log("Distance: "+distance);
 +
      $('#first').css({"transform": "translateX(0)", "-webkit-transform": "translateX(0)", "-ms-transform": "translateX(0)"}); /* 2 first */
 +
        $('#second').css({"z-index": "100"}); /* begin second */
 +
        $('#third').css({});
 +
        $('#fourth').css({});
 +
        $('#fifth').css({});
 +
        $('#sixth').css({});
 +
        $('#seventh').css({"z-index": "-100", "transform": "translateX(0)", "-webkit-transform": "translateX(0)", "-ms-transform": "translateX(0)"}); /* 4 seventh */
 +
        $('#eightth').css({"transform": "translateX(0)", "-webkit-transform": "translateX(0)", "-ms-transform": "translateX(0)"}); /* 3 eightth */
 +
        distance = 2;
 +
    }
 +
 
 +
    else if (distance ==2) {
 +
      console.log("Distance: "+distance);
 +
      $('#first').css({"transform": "translateX(1000px)", "-webkit-transform": "translateX(1000px)", "-ms-transform": "translateX(1000px)"}); /* 3 first */
 +
        $('#second').css({"transform": "translateX(1000px)", "-webkit-transform": "translateX(1000px)", "-ms-transform": "translateX(1000px)"}); /* 2 second */
 +
        $('#third').css({"z-index": "100"}); /* begin third */
 +
        $('#fourth').css({});
 +
        $('#fifth').css({});
 +
        $('#sixth').css({});
 +
        $('#seventh').css({});
 +
        $('#eightth').css({"z-index": "-100", "transform": "translateX(-2000px)", "-webkit-transform": "translateX(-2000px)", "-ms-transform": "translateX(-2000px)"}); /* 4 eightth */
 +
        distance = 3;
 +
    }
 +
 
 +
    else if (distance ==3) {
 +
      console.log("Distance: "+distance);
 +
      $('#first').css({"z-index": "-100", "transform": "translateX(-1000px)", "-webkit-transform": "translateX(-1000px)", "-ms-transform": "translateX(-1000px)", "-webkit-transform": "translateX(-1000px)", "-ms-transform": "translateX(-1000px)"}); /* 4 first */
 +
        $('#second').css({"transform": "translateX(2000px)", "-webkit-transform": "translateX(2000px)", "-ms-transform": "translateX(2000px)"}); /* 3 second */
 +
        $('#third').css({"transform": "translateX(1000px)", "-webkit-transform": "translateX(1000px)", "-ms-transform": "translateX(1000px)"}); /* 2 third */
 +
        $('#fourth').css({"z-index": "100"}); /* begin fourth */
 +
        $('#fifth').css({});
 +
        $('#sixth').css({});
 +
        $('#seventh').css({});
 +
        $('#eightth').css({});
 +
        distance = 4;
 +
    }
 +
 
 +
    else if (distance ==4) {
 +
      console.log("Distance: "+distance);
 +
      $('#first').css({});
 +
        $('#second').css({"z-index": "-100", "transform": "translateX(0)", "-webkit-transform": "translateX(0)", "-ms-transform": "translateX(0)"}); /* 4 second */
 +
        $('#third').css({"transform": "translateX(2000px)", "-webkit-transform": "translateX(2000px)", "-ms-transform": "translateX(2000px)"}); /* 3 third */
 +
        $('#fourth').css({"transform": "translateX(1000px)", "-webkit-transform": "translateX(1000px)", "-ms-transform": "translateX(1000px)"}); /* 2 fourth */
 +
        $('#fifth').css({"z-index": "100"}); /* begin fifth */
 +
        $('#sixth').css({});
 +
        $('#seventh').css({});
 +
        $('#eightth').css({});
 +
        distance = 5;
 +
    }
 +
 
 +
    else if (distance ==5) {
 +
      console.log("Distance: "+distance);
 +
      $('#first').css({});
 +
        $('#second').css({});
 +
        $('#third').css({"z-index": "-100", "transform": "translateX(0)", "-webkit-transform": "translateX(0)", "-ms-transform": "translateX(0)"}); /* 4 third */
 +
        $('#fourth').css({"transform": "translateX(2000px)", "-webkit-transform": "translateX(2000px)", "-ms-transform": "translateX(2000px)"}); /* 3 fourth */
 +
        $('#fifth').css({"transform": "translateX(1000px)", "-webkit-transform": "translateX(1000px)", "-ms-transform": "translateX(1000px)"}); /* 2 fifth */
 +
        $('#sixth').css({"z-index": "100"}); /* begin sixth */
 +
        $('#seventh').css({});
 +
        $('#eightth').css({});
 +
        distance = 6;
 +
    }
 +
 
 +
    else if (distance == 6) {
 +
      console.log("Distance: "+distance);
 +
      $('#first').css({});
 +
        $('#second').css({});
 +
        $('#third').css({});
 +
        $('#fourth').css({"z-index" : "-100", "transform": "translateX(0)", "-webkit-transform": "translateX(0)", "-ms-transform": "translateX(0)"}); /* 4 fourth */
 +
        $('#fifth').css({"transform": "translateX(2000px)", "-webkit-transform": "translateX(2000px)", "-ms-transform": "translateX(2000px)"}); /* 3 fifth */
 +
        $('#sixth').css({"transform": "translateX(1000px)", "-webkit-transform": "translateX(1000px)", "-ms-transform": "translateX(1000px)"}); /* 2 sixth */
 +
        $('#seventh').css({"z-index": "100"}); /* begin seventh */
 +
        $('#eightth').css({});
 +
        distance = 7;
 +
    }
 +
 
 +
    else if (distance == 7) {
 +
      console.log("Distance: "+distance);
 +
      $('#first').css({});
 +
        $('#second').css({});
 +
        $('#third').css({});
 +
        $('#fourth').css({});
 +
        $('#fifth').css({"z-index": "-100", "transform": "translateX(0)", "-webkit-transform": "translateX(0)", "-ms-transform": "translateX(0)"}); /* 4 fifth */
 +
        $('#sixth').css({"transform": "translateX(2000px)", "-webkit-transform": "translateX(2000px)", "-ms-transform": "translateX(2000px)"}); /* 3 sixth */
 +
        $('#seventh').css({"transform": "translateX(1000px)", "-webkit-transform": "translateX(1000px)", "-ms-transform": "translateX(1000px)"}); /* 2 seventh */
 +
        $('#eightth').css({"z-index": "100"}); /* begin eightth */
 +
        distance = 8;
 +
    }
 +
 
 +
    else if (distance == 8) {
 +
      console.log("Distance: "+distance);
 +
      $('#first').css({"z-index": "100"}); /* begin first */
 +
        $('#second').css({});
 +
        $('#third').css({});
 +
        $('#fourth').css({});
 +
        $('#fifth').css({});
 +
        $('#sixth').css({"z-index": "-100", "transform": "translateX(0)", "-webkit-transform": "translateX(0)", "-ms-transform": "translateX(0)"}); /* 4 sixth */
 +
        $('#seventh').css({"transform": "translateX(2000px)", "-webkit-transform": "translateX(2000px)", "-ms-transform": "translateX(2000px)"}); /* 3 seventh */
 +
        $('#eightth').css({"transform": "translateX(-1000px)", "-webkit-transform": "translateX(-1000px)", "-ms-transform": "translateX(-1000px)"}); /* 2 eightth */
 +
        distance = 1;
 +
    }
 +
 
 +
    // see: http://stackoverflow.com/questions/1836105/how-to-wait-5-seconds-with-jquery //
 +
    // wait until css-animation has finished. time is specified in css-transition property //
 +
    setTimeout(function(){
 +
      $("#buttonleft").bind("click", moveleft);
 +
    }, 1000);
 +
  }
 +
 
 +
 
 +
    $("#buttonright").bind("click", moveright);
 +
  function moveright(e) {
 +
 
 +
    $("#buttonright").unbind();
 +
    console.log("Click "+distance);
 +
 
 +
    if (distance == 1) {
 +
      $('#first').css({"z-index": "-100"}); /* 1 second */
 +
        $('#second').css({});
 +
        $('#third').css({});
 +
        $('#fourth').css({});
 +
        $('#fifth').css({});
 +
        $('#sixth').css({"transform": "translateX(2000px)", "-webkit-transform": "translateX(2000px)", "-ms-transform": "translateX(2000px)"}); /* 6 third */
 +
        $('#seventh').css({"z-index": "100","transform": "translateX(1000px)", "-webkit-transform": "translateX(1000px)", "-ms-transform": "translateX(1000px)"}); /* 7 third */
 +
        $('#eightth').css({"transform": "translateX(-2000px)", "-webkit-transform": "translateX(-2000px)", "-ms-transform": "translateX(-2000px)"}); /* 8 first */
 +
        distance = 8;
 +
    }
 +
 
 +
    else if (distance ==2) {
 +
      console.log("Distance: "+distance);
 +
      $('#first').css({"transform": "translateX(-1000px)", "-webkit-transform": "translateX(-1000px)", "-ms-transform": "translateX(-1000px)"}); /* 1 first */
 +
        $('#second').css({"z-index": "-100"}); /* 2 second */
 +
        $('#third').css({});
 +
        $('#fourth').css({});
 +
        $('#fifth').css({});
 +
        $('#sixth').css({});
 +
        $('#seventh').css({"transform": "translateX(2000px)", "-webkit-transform": "translateX(2000px)", "-ms-transform": "translateX(2000px)"}); /* 7 third */
 +
        $('#eightth').css({"z-index": "100","transform": "translateX(-1000px)", "-webkit-transform": "translateX(-1000px)", "-ms-transform": "translateX(-1000px)"}); /* 8 fourth */
 +
        distance = 1;
 +
    }
 +
 
 +
    else if (distance ==3) {
 +
      console.log("Distance: "+distance);
 +
      $('#first').css({"z-index": "100","transform": "translateX(0)", "-webkit-transform": "translateX(0)", "-ms-transform": "translateX(0)"}); /* 1 fourth */
 +
        $('#second').css({"transform": "translateX(0)", "-webkit-transform": "translateX(0)", "-ms-transform": "translateX(0)"}); /* 2 first */
 +
        $('#third').css({"z-index": "-100"}); /* 3 second */
 +
        $('#fourth').css({});
 +
        $('#fifth').css({});
 +
        $('#sixth').css({});
 +
        $('#seventh').css({});
 +
        $('#eightth').css({"transform": "translateX(0)", "-webkit-transform": "translateX(0)", "-ms-transform": "translateX(0)"}); /* 8 third */
 +
        distance = 2;
 +
    }
 +
 
 +
    else if (distance ==4) {
 +
      console.log("Distance: "+distance);
 +
      $('#first').css({"transform": "translateX(1000px)", "-webkit-transform": "translateX(1000px)", "-ms-transform": "translateX(1000px)"}); /* 1 third */
 +
        $('#second').css({"z-index": "100","transform": "translateX(1000px)", "-webkit-transform": "translateX(1000px)", "-ms-transform": "translateX(1000px)"}); /* 2 fourth */
 +
        $('#third').css({"transform": "translateX(0)", "-webkit-transform": "translateX(0)", "-ms-transform": "translateX(0)"}); /*3 first */
 +
        $('#fourth').css({"z-index": "-100"}); /* 4 second*/
 +
        $('#fifth').css({});
 +
        $('#sixth').css({});
 +
        $('#seventh').css({});
 +
        $('#eightth').css({});
 +
        distance = 3;
 +
    }
 +
 
 +
    else if (distance ==5) {
 +
      console.log("Distance: "+distance);
 +
      $('#first').css({});
 +
        $('#second').css({"transform": "translateX(2000px)", "-webkit-transform": "translateX(2000px)", "-ms-transform": "translateX(2000px)"}); /* 2 third */
 +
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 +
<div class="header_box">   
 +
<h1 class="headerbox">The DiaCHIP - A Versatile Detection System</h1>
 
</div>
 
</div>
−
<p>
+
 
−
The DiaCHIP is a new tool to simultaneously detect antibodies for different diseases in blood sera. Central to our project idea is the combination of antigen synthesis directly in the diagnostic device with a new and label-free detection system so simple that it can easily be rebuild by future iGEM teams.
+
<div class="float_barrier"></div>
−
</p>
+
 
 +
<div class="floatcontainer">
 +
 
 +
<div id='leftimage'>
 +
  <img align="left" alt="DiaCHIP_Sabi" src="https://static.igem.org/mediawiki/2015/a/af/Freiburg_DiaCHIP_Sabi.png" height="1430px">
 +
</div>
 +
 
 +
<div class="transparent_container">
 +
<div id="content_box1" class="content_box">
 +
<h2>Project Motivation</h2>
 +
<p>
 +
    Serological tests are a key element in modern medicine. Especially for detection and identification of infectious diseases, the performance of several blood tests is inevitable. Testing for more than one disease at once or diagnosing a patient with uncertain symptoms usually requires far more than one test. Every single test that is required increases the time of waiting for a precise diagnosis. In case of dangerous infectious diseases every minute until the onset of an appropriate treatment is crucial for the patient's survival. What if there was a possibility to combine all these tests in one single chip which offers a fast diagnosis and is affordable for everyone?
 +
</p>
 +
</div>
 +
</div>
 +
 
 +
 
 +
<div class="transparent_container">
 +
<div id="content_box2" class="content_box">
 +
<h2 style="text-align:left">Detecting Antigen-Antibody Interactions</h2>
 +
<p>
 +
The DiaCHIP is an innovative tool to screen for a broad range of antibodies in serum. Their presence serves as an indicator for an immune response towards an infectious disease or a successful vaccination. They also play an important role in the diagnosis of autoimmune diseases. Identifying diseases by detecting disease associated antibodies in a patient's serum is an established method in modern diagnostics. <br>
 +
Based on the very same principle, the DiaCHIP enables to screen for multiple diseases simultaneously, thereby reducing time and costs of a diagnosis. Especially the ability to differentiate between life threatening diseases and mild infections within a short time bears the potential to save lifes. 
 +
</p>
 +
</div>
 +
</div>
 +
 
 +
<div class="transparent_container">
 +
<div id="content_box3" class="content_box">
 +
<h2>The Concept</h2>
 +
<p>
 +
  The key feature of the DiaCHIP concept is the combination of on-demand protein synthesis and a novel method for label-free detection - all this packed into one device. The idea is to overcome challenges commonly found in protein array production and preservation. By cell-free expression of disease-related antigens, the protein array can be produced right when it is needed. In addition, results can be obtained in a time- and cost-efficient manner using a device simple enough to be rebuilt by future iGEM teams.
 +
</p>
 +
              <div class="link_button link_button_arrow">
 +
                <p><a href="https://2015.igem.org/Team:Freiburg/Project/System" title="System Overview">Step by Step Overview</a></p>
 +
              </div>
 +
</div>
 +
</div>
 +
 
 
</div>
 
</div>
−
<!-- EDIT1 SECTION "Project overview: The DiaCHIP" [1-351] -->
+
 
−
<div class="floatbox left">
+
<div class="float_barrier"></div>
−
<h2 class="sectionedit2">Preparing the DiaCHIP</h2>
+
 
−
<div class="level2">
+
 
−
<p>
+
 
−
As the DiaCHIP relies on antibody-antigen interaction the antigens first have to be synthesized and immobilized in the device. And as the whole device is a microfluidic system, it was most convenient to do this directly in the flow-chamber, where detection will finally happen.
+
<!---------------BEGIN SLIDER------------------>
−
The flow-chamber consists of two surfaces separated by a gap that can be flushed with liquids. On the upper surface DNA-molecules, providing the information for the antigens, are attached and can be transcribed and translated into protein when a cell-free expression mix is flushed through the gap. Proteins then diffuse downwards until reaching the second surface that specifically captures the proteins encoded by the immobilized DNA. After several washing steps to remove remaining expression-mix the flow-chamber is coated with antigens and ready for detection.  
+
<div class="container">
 +
  <div id="buttonright">
 +
    <img src="https://static.igem.org/mediawiki/2015/b/b3/Freiburg_lightbulb_slider_30.png">
 +
  </div>
 +
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 +
    <img src="https://static.igem.org/mediawiki/2015/4/4d/Freiburg_lightbulb_slider_27.png">
 +
  </div>
 +
  <div id="content-slider">
 +
      <div id="slider">  <!-- Slider container -->
 +
        <div id="mask">  <!-- Mask -->
 +
          <ul>
 +
 
 +
            <li id="first" class="firstanimation">  <!-- ID for tooltip and class for animation -->
 +
              <div class="artboard">
 +
              <div class="sliderimage" style="margin-top:10%">
 +
                <img src="https://static.igem.org/mediawiki/2015/6/6a/Freiburg_DiaCHIP_overview_scaled.jpg" width="350px">
 +
              </div>
 +
              <div class="slidertext">
 +
                <h1>The DiaCHIP - System Overview</h1>
 +
                <p>The core of our new diagnostic device consists of two slides that form a microfluidic chamber. Therein, an antigen array can be generated on demand by cell-free copying of a DNA template array. By flushing the chamber with a blood sample, antibodies present in the sample bind to corresponding antigens. This interaction is detected in real-time using the optical detection method iRIf.</p>
 +
                <p style="margin-top:10px">
 +
              <div class="intro_button menu-arrow">         
 +
                <a href="https://2015.igem.org/Team:Freiburg/Project/System" title="System Overview">Step by Step Overview</a>
 +
              </div>
 +
                </p>
 +
 
 +
              </div>
 +
              </div>
 +
            </li>
 +
 
 +
 
 +
            <li id="second" class="secondanimation">
 +
              <div class="artboard">
 +
              <div class="sliderimage" style="margin-top:12%">
 +
                <img src="https://static.igem.org/mediawiki/2015/9/94/Freiburg_furture_slider.png" width="350px">
 +
              </div>
 +
              <div class="slidertext">
 +
                <h1>Outlook</h1>
 +
                <p>Our results provide a proof of concept that the functional principle of the DiaCHIP is suitable for antibody detection in complex samples. Although further improvements have to be done in terms of reliablitiy and quantification, various additional applications are conceivable. Representing a way to reduce time and cost required for diagnosing a single patient, the DiaCHIP holds the potential to enhance and enlighten future diagnostics.  
 
</p>
 
</p>
−
</div>
+
            <p>
−
</div>
+
              <div class="intro_button menu-arrow">         
−
<!-- EDIT2 SECTION "Preparing the DiaCHIP" [352-1230] -->
+
                <a href="https://2015.igem.org/Team:Freiburg/Project/Future_Directions" title="Future_Directions">DiaCHIP in the <br>Future</a>
−
<div class="floatbox right">
+
              </div>
−
<h2 class="sectionedit3">Measuring</h2>
+
                </p>
−
<div class="level2">
+
            </li>
−
<p>
+
 
−
With the iRIf system it is possible to record small changes in layer thickness. The binding of an antibody from the blood serum flushed through the chamber increases the thickness of the lower surface so that interaction events can be measured label-free and in real-time.
+
 
 +
            <li id="third" class="thirdanimation">
 +
              <div class="artboard">
 +
              <div class="sliderimage" style="margin:6% auto">
 +
                <img src="https://static.igem.org/mediawiki/2015/d/db/Freiburg_iRiF_slider.png" width="70%">
 +
              </div>
 +
              <div class="slidertext">
 +
                <h1>Optical Detection: iRIf</h1>
 +
                <p>One disadvantage of currently available serological tests is the need for secondary labels that allow the detection of disease markers. Making use of an optical method based on the interference of light, the DiaCHIP can detect specific binding events on a protein microarray without further labeling. Read more about this innovative tool and the physics behind it.
 +
                </p>
 +
 
 +
                <p style="margin-top:15px">
 +
              <div class="intro_button menu-arrow">         
 +
                <a href="https://2015.igem.org/Team:Freiburg/Project/iRIf" title="Optical Detection">iRIf Principle and Physics</a>
 +
              </div>
 +
                </p>
 +
 
 +
              </div>
 +
              </div>
 +
            </li>
 +
 
 +
 
 +
            <li id="fourth" class="fourthanimation">
 +
              <div class="artboard">
 +
              <div class="sliderimage" style="margin:8% auto">
 +
                <img src="https://static.igem.org/mediawiki/2015/0/03/Freiburg_specific_surface_RJ_preview.jpg" width="230px">
 +
              </div>
 +
              <div class="slidertext">
 +
                <h1>Surface Chemistry</h1>
 +
                <p>The production of a customized protein microarray in the DiaCHIP is based on selective immobilization of antigens on a glass slide. Therefore, a specific surface chemistry was established to reduce the proportion of unspecific binding of non-target proteins to a minimum.
 +
                  Read more about the different layer compositions we tested on our way to high specificity.
 +
                </p>
 +
 
 +
                <p style="margin-top:15px">
 +
              <div class="intro_button menu-arrow">        
 +
                <a href="https://2015.igem.org/Team:Freiburg/Project/Surface_Chemistry" title="Surface Chemistry">Establishing a Specific Surface</a>
 +
              </div>
 +
                </p>
 +
 
 +
              </div>
 +
              </div>
 +
            </li>
 +
 
 +
 
 +
            <li id="fifth" class="fifthanimation">
 +
              <div class="artboard">
 +
              <div class="sliderimage" style="margin-top:10%">
 +
                <img src="https://static.igem.org/mediawiki/2015/f/f8/Freiburg_ProtPur_slider.png" width="350px">
 +
              </div>
 +
              <div class="slidertext">  
 +
                <h1>Protein Purification</h1>
 +
                <p>Protein expression in the DiaCHIP is mediated by cell-free expression. As this is an advanced method dependent on the optimization of many parameters, we got back to conventional protein purification in <i>E. coli</i> for being able to compare the results of both techniques. <br>
 +
Read more about overexpression and purification of several antigenic peptides. 
 +
                </p>
 +
 
 +
                <p style="margin-top:30px">
 +
              <div class="intro_button menu-arrow">         
 +
                <a href="https://2015.igem.org/Team:Freiburg/Project/Protein_Purification" title="Protein Purification">Purification of Antigens</a>
 +
              </div>
 +
                </p>
 +
 
 +
              </div>
 +
              </div>
 +
            </li>
 +
 
 +
 
 +
            <li id="sixth" class="sixthanimation">
 +
              <div class="artboard">
 +
              <div class="sliderimage" style="margin-top:12%">
 +
                <img src="https://static.igem.org/mediawiki/2015/e/eb/Freiburg_cellfreeexpressioninchamber.jpeg" width="350px">
 +
              </div>
 +
              <div class="slidertext">
 +
                <h1>Cell-Free Expression</h1>
 +
                <p> A key feature of the DiaCHIP is the capability to produce protein arrays on demand via cell-free expression. To reduce the cost of a DiaCHIP measurement, we produced a cell-free expression system based on an <i>E. coli</i> lysate ourselves. This system is also capable of expressing immobilized DNA sequences. See how this sensitive system was established and optimized.  
 
</p>
 
</p>
−
<p>
+
            <p style="margin-top:30px">
−
But we didn't stop at thinking about the device: <a href="https://2015.igem.org/Team:Freiburg/Results/Overview">We detected antibodies in our own blood!</a>
+
              <div class="intro_button menu-arrow">         
 +
                <a href="https://2015.igem.org/Team:Freiburg/Project/Cellfree_Expression" title="Cell-Free expression">Cell-free Antigen Expression</a>
 +
              </div>
 +
                </p>
 +
            </li>
 +
 
 +
 
 +
            <li id="seventh" class="seventhanimation">
 +
              <div class="artboard">
 +
              <div class="sliderimage" style="margin:10% 60px 3% 25px">
 +
                <img src="https://static.igem.org/mediawiki/2015/b/b2/Freiburg_DNAengineering_slider.png" width="350px">
 +
              </div>
 +
              <div class="slidertext">
 +
                <h1>DNA Engineering</h1>
 +
                <p>Genetic fusion of different antigens and tags is a basic requirement of our project. In order to enable several people to work in parallel we designed a cloning strategy easy to follow and additionally easy to expand for further needs.
 +
Read more about the combination of different cloning methods to reduce efforts in DNA Engineering and the design of an expression vector meeting iGEM requirements.
 
</p>
 
</p>
−
</div>
+
 
−
<div class="tags"><span>
+
                <p style="margin-top:30px">
−
<a class="wikilink1" href="/igem2015/doku.php?id=tag:info&amp;do=showtag&amp;tag=info" rel="tag" title="tag:info">info</a>
+
              <div class="intro_button menu-arrow">         
−
</span></div>
+
                <a href="https://2015.igem.org/Team:Freiburg/Methods/Cloning" title="DNA Engineering">DNA <br>Engineering</a>
−
</div>
+
              </div>
−
<!-- EDIT3 SECTION "Measuring" [1231-] -->
+
                </p>
 +
 
 +
              </div>
 +
              </div>
 +
            </li>
 +
 
 +
 
 +
            <li id="eightth" class="eightthanimation">
 +
              <div class="artboard">
 +
              <div class="sliderimage" style="margin:10% 60px 3% 25px">
 +
                <img src="https://static.igem.org/mediawiki/2015/0/0b/Freiburg_ELISA_slider.png" width="350px">
 +
              </div>
 +
              <div class="slidertext">
 +
                <h1>Diagnostics Today</h1>
 +
                <p>Currently used serological tests are available for a broad range of infectious diseases. However, they meet limitations that restrict an early onset of appropriate treatments which could be life-saving. The necessity of performing several tests to check for more than one disease is not only time-consuming but also costly. The DiaCHIP tackles these issues by providing a fast and affordable method for simultaneous testing.
 +
                </p>
 +
 
 +
                <p style="margin-top:30px">
 +
              <div class="intro_button menu-arrow">         
 +
                <a href="https://2015.igem.org/Team:Freiburg/Design" title="Diagnostics today">Limitations and Solutions</a>
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Latest revision as of 21:42, 18 September 2015

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The DiaCHIP - A Versatile Detection System

DiaCHIP_Sabi

Project Motivation

Serological tests are a key element in modern medicine. Especially for detection and identification of infectious diseases, the performance of several blood tests is inevitable. Testing for more than one disease at once or diagnosing a patient with uncertain symptoms usually requires far more than one test. Every single test that is required increases the time of waiting for a precise diagnosis. In case of dangerous infectious diseases every minute until the onset of an appropriate treatment is crucial for the patient's survival. What if there was a possibility to combine all these tests in one single chip which offers a fast diagnosis and is affordable for everyone?

Detecting Antigen-Antibody Interactions

The DiaCHIP is an innovative tool to screen for a broad range of antibodies in serum. Their presence serves as an indicator for an immune response towards an infectious disease or a successful vaccination. They also play an important role in the diagnosis of autoimmune diseases. Identifying diseases by detecting disease associated antibodies in a patient's serum is an established method in modern diagnostics.
Based on the very same principle, the DiaCHIP enables to screen for multiple diseases simultaneously, thereby reducing time and costs of a diagnosis. Especially the ability to differentiate between life threatening diseases and mild infections within a short time bears the potential to save lifes.

The Concept

The key feature of the DiaCHIP concept is the combination of on-demand protein synthesis and a novel method for label-free detection - all this packed into one device. The idea is to overcome challenges commonly found in protein array production and preservation. By cell-free expression of disease-related antigens, the protein array can be produced right when it is needed. In addition, results can be obtained in a time- and cost-efficient manner using a device simple enough to be rebuilt by future iGEM teams.

Step by Step Overview

  • The DiaCHIP - System Overview

    The core of our new diagnostic device consists of two slides that form a microfluidic chamber. Therein, an antigen array can be generated on demand by cell-free copying of a DNA template array. By flushing the chamber with a blood sample, antibodies present in the sample bind to corresponding antigens. This interaction is detected in real-time using the optical detection method iRIf.

    Step by Step Overview

  • Outlook

    Our results provide a proof of concept that the functional principle of the DiaCHIP is suitable for antibody detection in complex samples. Although further improvements have to be done in terms of reliablitiy and quantification, various additional applications are conceivable. Representing a way to reduce time and cost required for diagnosing a single patient, the DiaCHIP holds the potential to enhance and enlighten future diagnostics.

    DiaCHIP in the
    Future

  • Optical Detection: iRIf

    One disadvantage of currently available serological tests is the need for secondary labels that allow the detection of disease markers. Making use of an optical method based on the interference of light, the DiaCHIP can detect specific binding events on a protein microarray without further labeling. Read more about this innovative tool and the physics behind it.

    iRIf Principle and Physics

  • Surface Chemistry

    The production of a customized protein microarray in the DiaCHIP is based on selective immobilization of antigens on a glass slide. Therefore, a specific surface chemistry was established to reduce the proportion of unspecific binding of non-target proteins to a minimum. Read more about the different layer compositions we tested on our way to high specificity.

    Establishing a Specific Surface

  • Protein Purification

    Protein expression in the DiaCHIP is mediated by cell-free expression. As this is an advanced method dependent on the optimization of many parameters, we got back to conventional protein purification in E. coli for being able to compare the results of both techniques.
    Read more about overexpression and purification of several antigenic peptides.

    Purification of Antigens

  • Cell-Free Expression

    A key feature of the DiaCHIP is the capability to produce protein arrays on demand via cell-free expression. To reduce the cost of a DiaCHIP measurement, we produced a cell-free expression system based on an E. coli lysate ourselves. This system is also capable of expressing immobilized DNA sequences. See how this sensitive system was established and optimized.

    Cell-free Antigen Expression

  • DNA Engineering

    Genetic fusion of different antigens and tags is a basic requirement of our project. In order to enable several people to work in parallel we designed a cloning strategy easy to follow and additionally easy to expand for further needs. Read more about the combination of different cloning methods to reduce efforts in DNA Engineering and the design of an expression vector meeting iGEM requirements.

    DNA
    Engineering

  • Diagnostics Today

    Currently used serological tests are available for a broad range of infectious diseases. However, they meet limitations that restrict an early onset of appropriate treatments which could be life-saving. The necessity of performing several tests to check for more than one disease is not only time-consuming but also costly. The DiaCHIP tackles these issues by providing a fast and affordable method for simultaneous testing.

    Limitations and Solutions