Difference between revisions of "Team:Freiburg/Glossary"
| Line 48: | Line 48: | ||
<tr class="row3"> | <tr class="row3"> | ||
<td class="col1"> AIDS </td><td class="col0"> Acquired Immune Deficiency Syndrome, full-blown diseases after infection with HIV </td></tr> | <td class="col1"> AIDS </td><td class="col0"> Acquired Immune Deficiency Syndrome, full-blown diseases after infection with HIV </td></tr> | ||
| − | + | <tr class="row4"> | |
<td class="col1"> APTES </td><td class="col0"> 3-aminopropyltriethoxysilane, a silane we used to link PDITC to the hydroxyl groups on the plasma activated glass slide of our protein array </td></tr> | <td class="col1"> APTES </td><td class="col0"> 3-aminopropyltriethoxysilane, a silane we used to link PDITC to the hydroxyl groups on the plasma activated glass slide of our protein array </td></tr> | ||
| − | + | <tr class="row5"> | |
| + | <td class="col1"> cell-free expression </td><td class="col0"> expression performed outside a cell by using cell-lysate and additional components </td></tr> | ||
| + | <tr class="row6"> | ||
<td class="col1"> CFP </td><td class="col0"> cyan fluorescent protein </td></tr> | <td class="col1"> CFP </td><td class="col0"> cyan fluorescent protein </td></tr> | ||
| − | + | <tr class="row7"> | |
| + | <td class="col1"> chlamydia </td><td class="col0"> sexually transmittable disease, transmitted by the bacterium <i>Chlamydia trachomatis</i> </td></tr> | ||
| + | <tr class="col8"> | ||
<td class="col1"> classical cloning </td><td class="col0"> insertion of a DNA fragment for example into a plasmid backbone via restriction enzymes; each fragment has to be inserted separately. For differences to Gibson cloning see <a href="https://2015.igem.org/Team:Freiburg/Project/Classic_vs_Gibson"> here </a> </td></tr> | <td class="col1"> classical cloning </td><td class="col0"> insertion of a DNA fragment for example into a plasmid backbone via restriction enzymes; each fragment has to be inserted separately. For differences to Gibson cloning see <a href="https://2015.igem.org/Team:Freiburg/Project/Classic_vs_Gibson"> here </a> </td></tr> | ||
| − | + | <tr class="row9"> | |
| − | <td class="col1"> Cy3 </td><td class="col0"> Cyanine 3, | + | <td class="col1"> CrPV IRES </td><td class="col0"> Cricket Paralysis Virus internal ribosome entry site, forming a specific secondary structure allowing the entry of ribosomes in eucaryotes </td></tr> |
| − | + | <tr class="row10"> | |
| + | <td class="col1"> Cy3 </td><td class="col0"> Cyanine 3, fluorescent dye used particularly in biomedical imaging </td></tr> | ||
| + | <tr class="row11"> | ||
<td class="col1"> DE3 </td><td class="col0"> nomenclature of <a href="https://2015.igem.org/Team:Freiburg/Project/Coli_Strains"target="_blank"><i>E.coli</i> strains</a>, meaning it contains a T7 RNA-polymerase for general protein expression </td></tr> | <td class="col1"> DE3 </td><td class="col0"> nomenclature of <a href="https://2015.igem.org/Team:Freiburg/Project/Coli_Strains"target="_blank"><i>E.coli</i> strains</a>, meaning it contains a T7 RNA-polymerase for general protein expression </td></tr> | ||
| − | + | <tr class="row12"> | |
| + | <td class="col1"> DiaMIX </td><td class="col0"> the cell-free expression mix we produced ourselves, also providing the <a href="https://2015.igem.org/Team:Freiburg/Protocol#cell-free_protocol">protocol</a> </td></tr> | ||
| + | <tr class="row13"> | ||
<td class="col1"> DMSO </td><td class="col0"> Dimethylsulfoxid, added to PCR reactions </td></tr> | <td class="col1"> DMSO </td><td class="col0"> Dimethylsulfoxid, added to PCR reactions </td></tr> | ||
| − | + | <tr class="row14"> | |
<td class="col1"> dNTP </td><td class="col0"> deoxynucleotidetriphosphate, component of DNA and therefore added to PCR reaction mixes </td></tr> | <td class="col1"> dNTP </td><td class="col0"> deoxynucleotidetriphosphate, component of DNA and therefore added to PCR reaction mixes </td></tr> | ||
| − | + | <tr class="row15"> | |
<td class="col1"> Dot Blot </td><td class="col0"> simplification of Northern, Southern or Western Blot in order to save time; proteins are not separated electrophoretically but simply spotted on a membrane and then detected via nucleotide probes (Northern and Southern Blot) or antibodies (Western Blot) </td></tr> | <td class="col1"> Dot Blot </td><td class="col0"> simplification of Northern, Southern or Western Blot in order to save time; proteins are not separated electrophoretically but simply spotted on a membrane and then detected via nucleotide probes (Northern and Southern Blot) or antibodies (Western Blot) </td></tr> | ||
| − | + | <tr class="row16"> | |
<td class="col1"> ELISA </td><td class="col0"> enzyme-linked immunosorbent assay, antigens are immobilized in a 96-well plate, antibody-antigen-interaction is detected; find more information <a href="https://2015.igem.org/Team:Freiburg/Project/Diagnostics"> here </a> </td></tr> | <td class="col1"> ELISA </td><td class="col0"> enzyme-linked immunosorbent assay, antigens are immobilized in a 96-well plate, antibody-antigen-interaction is detected; find more information <a href="https://2015.igem.org/Team:Freiburg/Project/Diagnostics"> here </a> </td></tr> | ||
| − | + | <tr class="row17"> | |
<td class="col1"> fluorophore </td><td class="col0">chemical compound that can emit light when excitated with light of a certain wavelengh </td></tr> | <td class="col1"> fluorophore </td><td class="col0">chemical compound that can emit light when excitated with light of a certain wavelengh </td></tr> | ||
| − | + | <tr class="row18"> | |
<td class="col1"> GFP </td><td class="col0"> green fluorescent protein </td></tr> | <td class="col1"> GFP </td><td class="col0"> green fluorescent protein </td></tr> | ||
| − | + | <tr class="row19"> | |
<td class="col1"> Gibson Assembly </td><td class="col0"> combination of DNA fragments in an isothermal reaction without the need of restriction enzymes; more information and a comparison to classical cloning can be found <a href="https://2015.igem.org/Team:Freiburg/Project/Classic_vs_Gibson"> here </a> </td></tr> | <td class="col1"> Gibson Assembly </td><td class="col0"> combination of DNA fragments in an isothermal reaction without the need of restriction enzymes; more information and a comparison to classical cloning can be found <a href="https://2015.igem.org/Team:Freiburg/Project/Classic_vs_Gibson"> here </a> </td></tr> | ||
| − | + | <tr class="row20"> | |
<td class="col1"> glycoprotein </td><td class="col0"> surface protein with oligosaccharide chains covalently bound to the polypeptide; also present on virus surfaces and often provoking an immunogenic response in humans </td></tr> | <td class="col1"> glycoprotein </td><td class="col0"> surface protein with oligosaccharide chains covalently bound to the polypeptide; also present on virus surfaces and often provoking an immunogenic response in humans </td></tr> | ||
| − | + | <tr class="row21"> | |
| + | <td class="col1"> gonorrhea </td><td class="col0"> sexually transmittable disease, also known as tripper, transmitted via the bacterium <i>Neisseria gonorrhoeae</i> </td></tr> | ||
| + | <tr class="row22"> | ||
<td class="col1"> HA-tag </td><td class="col0"> tag derived from the human influenza hemagglutinin and extensively used as a general epitope tag in expression vectors. </td></tr> | <td class="col1"> HA-tag </td><td class="col0"> tag derived from the human influenza hemagglutinin and extensively used as a general epitope tag in expression vectors. </td></tr> | ||
| − | + | <tr class="row23"> | |
| + | <td class="col1"> HEPES-KOH </td><td class="col0"> 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid potassium hydroxide, component in a buffer widely used in cell culture </td></tr> | ||
| + | <tr class="row24"> | ||
<td class="col1"> Her2 protein </td><td class="col0"> biomarker for breast cancer </td></tr> | <td class="col1"> Her2 protein </td><td class="col0"> biomarker for breast cancer </td></tr> | ||
| − | + | <tr class="row25"> | |
<td class="col1"> His-tag </td><td class="col0"> tag consisting of several histidine residues binding to Ni-NTA and often used for protein purification and specific binding to surfaces </td></tr> | <td class="col1"> His-tag </td><td class="col0"> tag consisting of several histidine residues binding to Ni-NTA and often used for protein purification and specific binding to surfaces </td></tr> | ||
| − | + | <tr class="row26"> | |
<td class="col1"> HIV </td><td class="col0"> Human Immunodeficiency Virus, causes AIDS </td></tr> | <td class="col1"> HIV </td><td class="col0"> Human Immunodeficiency Virus, causes AIDS </td></tr> | ||
| − | + | <tr class="row27"> | |
<td class="col1"> HSV-1 </td><td class="col0"> Herpes Simplex Virus type 1, also known as Human Herpes Virus type 3 </td></tr> | <td class="col1"> HSV-1 </td><td class="col0"> Herpes Simplex Virus type 1, also known as Human Herpes Virus type 3 </td></tr> | ||
| − | + | <tr class="row28"> | |
<td class="col1"> IPTG </td><td class="col0"> Isopropylthiogalactopyranosid, allolactose analogue used to induce the lacI system in cells; often used to induce protein overexpression in bacteria </td></tr> | <td class="col1"> IPTG </td><td class="col0"> Isopropylthiogalactopyranosid, allolactose analogue used to induce the lacI system in cells; often used to induce protein overexpression in bacteria </td></tr> | ||
| − | + | <tr class="row29"> | |
| + | <td class="col1"> IRES </td><td class="col0"> internal ribosome entry site, forming a specific secondary structure allowing the entry of ribosomes in procaryotes </td></tr> | ||
| + | <tr class="row30"> | ||
<td class="col1"> iRIf </td><td class="col0"> imaging Reflectometric Interference, label-free detection method for protein-protein interactions; mode of operation and further information can be found <a href="https://2015.igem.org/Team:Freiburg/Project/iRIf">here</a> </td></tr> | <td class="col1"> iRIf </td><td class="col0"> imaging Reflectometric Interference, label-free detection method for protein-protein interactions; mode of operation and further information can be found <a href="https://2015.igem.org/Team:Freiburg/Project/iRIf">here</a> </td></tr> | ||
| − | + | <tr class="row31"> | |
<td class="col1"> LB </td><td class="col0"> Lyria-Bertani-medium, a nutritionally rich medium, primarily used for growing bacteria </td></tr> | <td class="col1"> LB </td><td class="col0"> Lyria-Bertani-medium, a nutritionally rich medium, primarily used for growing bacteria </td></tr> | ||
| − | + | <tr class="row32"> | |
| + | <td class="col1"> LOC </td><td class="col0"> Lab-on-a-Chip system, microarray based device for immunodiagnosis </td></tr> | ||
| + | <tr class="row33"> | ||
<td class="col1"> Mg(OAc)<sub>2</sub> </td><td class="col0"> magnesium acetate, used to supplement our cell-free expression mix</td></tr> | <td class="col1"> Mg(OAc)<sub>2</sub> </td><td class="col0"> magnesium acetate, used to supplement our cell-free expression mix</td></tr> | ||
| − | + | <tr class="row34"> | |
<td class="col1"> microfluidic chamber </td><td class="col0"> small compartment in a microfluidic device through which liquids in μL range can be rinsed </td></tr> | <td class="col1"> microfluidic chamber </td><td class="col0"> small compartment in a microfluidic device through which liquids in μL range can be rinsed </td></tr> | ||
| − | + | <tr class="row35"> | |
<td class="col1"> microfluidics </td><td class="col0"> system through which very small volumes of liquids can be channeled and processed</td></tr> | <td class="col1"> microfluidics </td><td class="col0"> system through which very small volumes of liquids can be channeled and processed</td></tr> | ||
| − | + | <tr class="row36"> | |
<td class="col1"> Ni-NTA </td><td class="col0"> nickel-nitrolotriacetic acid, chelate used for protein purification via a His-tag or as surface for immobilization of His-tagged proteins </td></tr> | <td class="col1"> Ni-NTA </td><td class="col0"> nickel-nitrolotriacetic acid, chelate used for protein purification via a His-tag or as surface for immobilization of His-tagged proteins </td></tr> | ||
| − | + | <tr class="row37"> | |
<td class="col1"> o/n </td><td class="col0"> overnight, used in the labjournals as abbreviation </td></tr> | <td class="col1"> o/n </td><td class="col0"> overnight, used in the labjournals as abbreviation </td></tr> | ||
| − | + | <tr class="row38"> | |
<td class="col1"> OD </td><td class="col0"> optical density, used to determine approximate cell density in a solution </td></tr> | <td class="col1"> OD </td><td class="col0"> optical density, used to determine approximate cell density in a solution </td></tr> | ||
| − | + | <tr class="row39"> | |
<td class="col1"> PCR </td><td class="col0"> polymerase chain reaction, used to amplify DNA fragments using specific primers </td></tr> | <td class="col1"> PCR </td><td class="col0"> polymerase chain reaction, used to amplify DNA fragments using specific primers </td></tr> | ||
| − | + | <tr class="row40"> | |
<td class="col1"> PDITC </td><td class="col0"> p-Phenyldiisothiocyanate, used to link amino groups, in our project used to produce a surface to which proteins can bind, e.g. on a glass slide </td></tr> | <td class="col1"> PDITC </td><td class="col0"> p-Phenyldiisothiocyanate, used to link amino groups, in our project used to produce a surface to which proteins can bind, e.g. on a glass slide </td></tr> | ||
| − | + | <tr class="row41"> | |
<td class="col1"> PDMS </td><td class="col0"> Polydimethylsiloxane, in our project part of the DiaCHIP forming the basis to immobilize DNA fragments as template for cell-free expression </td></tr> | <td class="col1"> PDMS </td><td class="col0"> Polydimethylsiloxane, in our project part of the DiaCHIP forming the basis to immobilize DNA fragments as template for cell-free expression </td></tr> | ||
| − | + | <tr class="row42"> | |
<td class="col1"> pelB </td><td class="col0"> signal sequence for periplasmic translocation of a protein </td></tr> | <td class="col1"> pelB </td><td class="col0"> signal sequence for periplasmic translocation of a protein </td></tr> | ||
| − | + | <tr class="row43"> | |
<td class="col1"> pET22b+ </td><td class="col0"> commonly used plasmid for protein overexpression in bacteria </td></tr> | <td class="col1"> pET22b+ </td><td class="col0"> commonly used plasmid for protein overexpression in bacteria </td></tr> | ||
| − | + | <tr class="row44"> | |
<td class="col1"> pJet </td><td class="col0"> blunt-end cloning vector </td> </tr> | <td class="col1"> pJet </td><td class="col0"> blunt-end cloning vector </td> </tr> | ||
| − | + | <tr class="row45"> | |
<td class="col1"> plasmid </td><td class="col0"> small, circular DNA molecule </td></tr> | <td class="col1"> plasmid </td><td class="col0"> small, circular DNA molecule </td></tr> | ||
| − | + | <tr class="row46"> | |
<td class="col1"> PMSF </td><td class="col0"> Phenylmethylsulfonylfluorid, small organic molecule that specifically inhibits serine proteases to prevent protein degradation in a disrupted cell </td></tr> | <td class="col1"> PMSF </td><td class="col0"> Phenylmethylsulfonylfluorid, small organic molecule that specifically inhibits serine proteases to prevent protein degradation in a disrupted cell </td></tr> | ||
| − | + | <tr class="row47"> | |
| + | <td class="col1"> POC </td><td class="col0"> Point-of-Care diagnostics, diagnostic devices that can be used at home or even on the bedside of a patient </td></tr> | ||
| + | <tr class="row48"> | ||
<td class="col1"> pOP </td><td class="col0"> plasmid for Overexpression of Proteins, new plasmid backbone we submitted to the iGEM Registry, find out all the details <a href="https://2015.igem.org/Team:Freiburg/Project/pOP-vector"> here </a> </td></tr> | <td class="col1"> pOP </td><td class="col0"> plasmid for Overexpression of Proteins, new plasmid backbone we submitted to the iGEM Registry, find out all the details <a href="https://2015.igem.org/Team:Freiburg/Project/pOP-vector"> here </a> </td></tr> | ||
| − | + | <tr class="row49"> | |
<td class="col1"> Quotient picture </td><td class="col0"> picture taken after a particular step devided by one taken after the step, important for the evaluation of the iRIf measurements </td></tr> | <td class="col1"> Quotient picture </td><td class="col0"> picture taken after a particular step devided by one taken after the step, important for the evaluation of the iRIf measurements </td></tr> | ||
| − | + | <tr class="row50"> | |
<td class="col1"> RT </td><td class="col0"> room temperature, abbreviation used in the labjournal </td></tr> | <td class="col1"> RT </td><td class="col0"> room temperature, abbreviation used in the labjournal </td></tr> | ||
| − | + | <tr class="row51"> | |
<td class="col1"> RV </td><td class="col0"> Rubella Virus, causes German measles </td></tr> | <td class="col1"> RV </td><td class="col0"> Rubella Virus, causes German measles </td></tr> | ||
| − | + | <tr class="row52"> | |
<td class="col1"> SDS-PAGE </td><td class="col0"> sodium dodecyl sulfate polyacrylamide gel electrophoresis, used to separate and analyze proteins according to their molecular mass within an electric field </td></tr> | <td class="col1"> SDS-PAGE </td><td class="col0"> sodium dodecyl sulfate polyacrylamide gel electrophoresis, used to separate and analyze proteins according to their molecular mass within an electric field </td></tr> | ||
| − | + | <tr class="row53"> | |
| + | <td class="col1"> shine dalgarno sequence </td><td class="col0"> sequence in bacterial mRNA that is recognized as a part of the ribosomal binding site </td></tr> | ||
| + | <tr class="row54"> | ||
<td class="col1"> SpyCatcher </td><td class="col0"> protein that forms a covalent bond with the Spy-tag, system we tried to establish a specific surface for the protein microarray </td></tr> | <td class="col1"> SpyCatcher </td><td class="col0"> protein that forms a covalent bond with the Spy-tag, system we tried to establish a specific surface for the protein microarray </td></tr> | ||
| − | + | <tr class="row55"> | |
<td class="col1"> SpyTag </td><td class="col0"> tag that can be genetically fused to proteins for subsequent covalent bonding with the Spy-catcher </td></tr> | <td class="col1"> SpyTag </td><td class="col0"> tag that can be genetically fused to proteins for subsequent covalent bonding with the Spy-catcher </td></tr> | ||
| − | + | <tr class="row56"> | |
<td class="col1"> TAE </td><td class="col0"> TRIS-acetic acid-EDTA mix used in buffers for gel electrophoresis </td></tr> | <td class="col1"> TAE </td><td class="col0"> TRIS-acetic acid-EDTA mix used in buffers for gel electrophoresis </td></tr> | ||
| − | + | <tr class="row57"> | |
| + | <td class="col1"> trichomoniasis </td><td class="col0"> sexually transmittable disease, transmitted via the flagellate <i>Trichomonas vaginalis</i> </td></tr> | ||
| + | <tr class="row58"> | ||
<td class="col1"> turboYFP </td><td class="col0"> enhanced version of the yellow fluorescent protein </td></tr> | <td class="col1"> turboYFP </td><td class="col0"> enhanced version of the yellow fluorescent protein </td></tr> | ||
| − | + | <tr class="row59"> | |
<td class="col1"> VZV </td><td class="col0"> Varicella Zoster Virus, also known as Human Herpes Virus type 6, causes chickenpox </td></tr> | <td class="col1"> VZV </td><td class="col0"> Varicella Zoster Virus, also known as Human Herpes Virus type 6, causes chickenpox </td></tr> | ||
| − | + | <tr class="row60"> | |
<td class="col1"> Western Blot </td><td class="col0"> analytical technique used to detect specific proteins by using specific antibodies after separation of proteins by gel electrophoresis </td></tr> | <td class="col1"> Western Blot </td><td class="col0"> analytical technique used to detect specific proteins by using specific antibodies after separation of proteins by gel electrophoresis </td></tr> | ||
Revision as of 21:56, 18 September 2015
Glossary
This glossary is meant to help you with the understanding of our whole website and therefore of our project. We provide you with a short overview of the most important terms, techniques, devices and abbreviations you might encounter while combing through our information pages and labjournals.
| term | definition |
|---|---|
| 5’UTR | an untranslated region at the 5’ end of the RNA sequence that enhances translation |
| affibody | antibody mimetic, small protein engineered to bind to a large number of target proteins or peptides with high affinity, imitating monoclonal antibodies |
| AIDS | Acquired Immune Deficiency Syndrome, full-blown diseases after infection with HIV |
| APTES | 3-aminopropyltriethoxysilane, a silane we used to link PDITC to the hydroxyl groups on the plasma activated glass slide of our protein array |
| cell-free expression | expression performed outside a cell by using cell-lysate and additional components |
| CFP | cyan fluorescent protein |
| chlamydia | sexually transmittable disease, transmitted by the bacterium Chlamydia trachomatis |
| classical cloning | insertion of a DNA fragment for example into a plasmid backbone via restriction enzymes; each fragment has to be inserted separately. For differences to Gibson cloning see here |
| CrPV IRES | Cricket Paralysis Virus internal ribosome entry site, forming a specific secondary structure allowing the entry of ribosomes in eucaryotes |
| Cy3 | Cyanine 3, fluorescent dye used particularly in biomedical imaging |
| DE3 | nomenclature of E.coli strains, meaning it contains a T7 RNA-polymerase for general protein expression |
| DiaMIX | the cell-free expression mix we produced ourselves, also providing the protocol |
| DMSO | Dimethylsulfoxid, added to PCR reactions |
| dNTP | deoxynucleotidetriphosphate, component of DNA and therefore added to PCR reaction mixes |
| Dot Blot | simplification of Northern, Southern or Western Blot in order to save time; proteins are not separated electrophoretically but simply spotted on a membrane and then detected via nucleotide probes (Northern and Southern Blot) or antibodies (Western Blot) |
| ELISA | enzyme-linked immunosorbent assay, antigens are immobilized in a 96-well plate, antibody-antigen-interaction is detected; find more information here |
| fluorophore | chemical compound that can emit light when excitated with light of a certain wavelengh |
| GFP | green fluorescent protein |
| Gibson Assembly | combination of DNA fragments in an isothermal reaction without the need of restriction enzymes; more information and a comparison to classical cloning can be found here |
| glycoprotein | surface protein with oligosaccharide chains covalently bound to the polypeptide; also present on virus surfaces and often provoking an immunogenic response in humans |
| gonorrhea | sexually transmittable disease, also known as tripper, transmitted via the bacterium Neisseria gonorrhoeae |
| HA-tag | tag derived from the human influenza hemagglutinin and extensively used as a general epitope tag in expression vectors. |
| HEPES-KOH | 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid potassium hydroxide, component in a buffer widely used in cell culture |
| Her2 protein | biomarker for breast cancer |
| His-tag | tag consisting of several histidine residues binding to Ni-NTA and often used for protein purification and specific binding to surfaces |
| HIV | Human Immunodeficiency Virus, causes AIDS |
| HSV-1 | Herpes Simplex Virus type 1, also known as Human Herpes Virus type 3 |
| IPTG | Isopropylthiogalactopyranosid, allolactose analogue used to induce the lacI system in cells; often used to induce protein overexpression in bacteria |
| IRES | internal ribosome entry site, forming a specific secondary structure allowing the entry of ribosomes in procaryotes |
| iRIf | imaging Reflectometric Interference, label-free detection method for protein-protein interactions; mode of operation and further information can be found here |
| LB | Lyria-Bertani-medium, a nutritionally rich medium, primarily used for growing bacteria |
| LOC | Lab-on-a-Chip system, microarray based device for immunodiagnosis |
| Mg(OAc)2 | magnesium acetate, used to supplement our cell-free expression mix |
| microfluidic chamber | small compartment in a microfluidic device through which liquids in μL range can be rinsed |
| microfluidics | system through which very small volumes of liquids can be channeled and processed |
| Ni-NTA | nickel-nitrolotriacetic acid, chelate used for protein purification via a His-tag or as surface for immobilization of His-tagged proteins |
| o/n | overnight, used in the labjournals as abbreviation |
| OD | optical density, used to determine approximate cell density in a solution |
| PCR | polymerase chain reaction, used to amplify DNA fragments using specific primers |
| PDITC | p-Phenyldiisothiocyanate, used to link amino groups, in our project used to produce a surface to which proteins can bind, e.g. on a glass slide |
| PDMS | Polydimethylsiloxane, in our project part of the DiaCHIP forming the basis to immobilize DNA fragments as template for cell-free expression |
| pelB | signal sequence for periplasmic translocation of a protein |
| pET22b+ | commonly used plasmid for protein overexpression in bacteria |
| pJet | blunt-end cloning vector |
| plasmid | small, circular DNA molecule |
| PMSF | Phenylmethylsulfonylfluorid, small organic molecule that specifically inhibits serine proteases to prevent protein degradation in a disrupted cell |
| POC | Point-of-Care diagnostics, diagnostic devices that can be used at home or even on the bedside of a patient |
| pOP | plasmid for Overexpression of Proteins, new plasmid backbone we submitted to the iGEM Registry, find out all the details here |
| Quotient picture | picture taken after a particular step devided by one taken after the step, important for the evaluation of the iRIf measurements |
| RT | room temperature, abbreviation used in the labjournal |
| RV | Rubella Virus, causes German measles |
| SDS-PAGE | sodium dodecyl sulfate polyacrylamide gel electrophoresis, used to separate and analyze proteins according to their molecular mass within an electric field |
| shine dalgarno sequence | sequence in bacterial mRNA that is recognized as a part of the ribosomal binding site |
| SpyCatcher | protein that forms a covalent bond with the Spy-tag, system we tried to establish a specific surface for the protein microarray |
| SpyTag | tag that can be genetically fused to proteins for subsequent covalent bonding with the Spy-catcher |
| TAE | TRIS-acetic acid-EDTA mix used in buffers for gel electrophoresis |
| trichomoniasis | sexually transmittable disease, transmitted via the flagellate Trichomonas vaginalis |
| turboYFP | enhanced version of the yellow fluorescent protein |
| VZV | Varicella Zoster Virus, also known as Human Herpes Virus type 6, causes chickenpox |
| Western Blot | analytical technique used to detect specific proteins by using specific antibodies after separation of proteins by gel electrophoresis |
