Difference between revisions of "Team:Pretoria UP"

 
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<img id="Banner" alt="Banner By Natascha Muller" src="/wiki/images/0/03/Banner_Pretoria_UP.png" style="width:30%; padding-top:7.5%;"/>
  
  
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<h4 id="description">SYNCHRONIZED, CONDITIONAL, IRREVERSIBLE CHEMOTAXIS PROGRAMMING...</h4>
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<!--<h4 id="description">SYNCHRONIZED, CONDITIONAL, GENETIC CHEMOTAXIS PROGRAMMING</h4>-->
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                <h2 id="description"> Synchronized, Conditional, Genetic Chemotaxis Programming  </h2>
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<img id="animation" alt="Animation by Schae Ind" src="/wiki/images/1/15/Up_animation.gif"/>
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<div align="center" style="width:100%; padding-right:6%;">
 
<div align="center" style="width:100%; padding-right:6%;">
 
<h3 style="clear:both;"> Project Description </h3>
 
<h3 style="clear:both;"> Project Description </h3>
  
 
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We decided to improve the 2011 WITS-CSIR_SA “biotweet” project based on chemotaxis reversal in semi-solid media using riboswitches. The Pretoria_UP project “Switch coli” enhances several limitations on the original design, incorporating quorum sensing, an “AND” logic gate, alternative designs for a recombination-based toggle switch, and an irreversible reporter system for detecting chemicals of interest. The enhanced prototype would allow for motile bacteria to “tweet” information to a destination, and for a reply tweet with new information on the state of the destination to be sent back in a synchronized, conditional manner.
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The Pretoria UP iGEM team of 2015 have proposed a design for intelligent motile bacteria based on a combination of DNA modules that confer different functions. The synchronous behaviour of the bacteria will be achieved through a quorum sensing module allowing the population to behave as a swarm instead of independent units. A conditional response to an environmental signal is conferred through a post transcriptional control mechanism known as riboswitching, thus the bacterial swarm may recognise and respond to a chemical attractant. Since the system makes use of several modular components, an AND gate is required to process the various signals which in turn would trigger a genetic switch in the bacterial chemotaxis. The irreversible change is programmed by a DNA recombination switch which inverts the directionality of a promoter through the Cre-loxP pathway. We invite you to explore the various aspects of the project on this site.
 
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</p>
  

Latest revision as of 22:00, 18 September 2015

Synchronized, Conditional, Genetic Chemotaxis Programming

Animation by Schae Ind

Project Description

The Pretoria UP iGEM team of 2015 have proposed a design for intelligent motile bacteria based on a combination of DNA modules that confer different functions. The synchronous behaviour of the bacteria will be achieved through a quorum sensing module allowing the population to behave as a swarm instead of independent units. A conditional response to an environmental signal is conferred through a post transcriptional control mechanism known as riboswitching, thus the bacterial swarm may recognise and respond to a chemical attractant. Since the system makes use of several modular components, an AND gate is required to process the various signals which in turn would trigger a genetic switch in the bacterial chemotaxis. The irreversible change is programmed by a DNA recombination switch which inverts the directionality of a promoter through the Cre-loxP pathway. We invite you to explore the various aspects of the project on this site.