Difference between revisions of "Team:British Columbia/Notebook/Protocols"

 
(32 intermediate revisions by 3 users not shown)
Line 1: Line 1:
 
{{:Team:British Columbia/Template/Fixednavbar}}
 
{{:Team:British Columbia/Template/Fixednavbar}}
 +
{{:Team:British Columbia/Notebook/ProtocolsStyles}}
 
<html lang="en">
 
<html lang="en">
−
+
<style>
 +
.hexagon{
 +
padding-top:10px;
 +
}
 +
.nav-pills>li.active>a, .nav-pills>li.active>a:focus, .nav-pills>li.active>a:hover{
 +
background-color:#e1d1b4;
 +
}
 +
a:visited, a{
 +
color:grey;
 +
}
 +
 
 +
</style>
 
   <body>
 
   <body>
 
<div class="bod">
 
<div class="bod">
Line 13: Line 25:
 
<div style="clear: both;"></div>
 
<div style="clear: both;"></div>
 
<div id="UBCbody">
 
<div id="UBCbody">
−
<h1><small>Use the categories on the left to find the protocol you need:</small></h1>
+
<h1><small>Select one of the categories below to find the protocol you need:</small></h1>
  
 
<div class="container">
 
<div class="container">
Line 22: Line 34:
 
             <div class="tabbable">
 
             <div class="tabbable">
 
                 <ul class="nav nav-pills nav-stacked col-md-2" style="width:1000px;">
 
                 <ul class="nav nav-pills nav-stacked col-md-2" style="width:1000px;">
−
                     <li style="float:left;"><a href="#a" data-toggle="tab"><strong>Growth</strong></a></li>
+
                     <li style="text-align: left;"><a href="#a" data-toggle="tab"><strong>Genetic Tool Development</strong></a></li>
−
                     <li style="float:left;"><a href="#b" data-toggle="tab">Imidacloprid Modification</a></li>
+
                     <li style="text-align: center;"><a href="#b" data-toggle="tab"><strong>Imidacloprid Modification</strong></a></li>
−
                     <li style="float:left;"><a href="#c" data-toggle="tab">6-CNA Degradation</a></li>
+
                     <li style="text-align: right;"><a href="#c" data-toggle="tab"><strong>6-CNA Degradation</strong></a></li>
−
                    <li style="float:left;"><a href="#d" data-toggle="tab">Screening</a></li>
+
 
                 </ul>
 
                 </ul>
−
                 <div class="tab-content col-md-9">
+
                 <div class="tab-content col-md-9" style="width:1000px; text-indent:0;">
−
                   
+
  
−
<div class="tab-pane" id="a"><div class="panel-group" id="accordion" role="tablist" aria-multiselectable="true">
+
<div class="tab-pane" id="a">
−
      <div class="panel panel-default">
+
 
−
        <div class="panel-heading" role="tab" id="headingOne">
+
<a href="#"><div class="hexagon"> <a href="https://static.igem.org/mediawiki/2015/1/15/UBC_growinggapicolaprotocol.pdf"><h4>Growing<br /> <i>G. apicola</i></h4></a>
−
          <h4 id="-collapsible-group-item-#1-" class="panel-title">
+
<div class="face1"></div>
−
            <a class="collapsed" data-toggle="collapse" data-parent="#accordion" href="#collapseOne" aria-expanded="false" aria-controls="collapseOne">
+
  <div class="face2"></div>
−
              <strong>Colony PCR DNA Amplification</strong>  
+
</div></a>
−
            </a>
+
 
−
          <a class="anchorjs-link" href="#-collapsible-group-item-#1-"><span class="anchorjs-icon"></span></a></h4>
+
<a href="#"><div class="hexagon"> <a href="https://static.igem.org/mediawiki/2015/1/1f/UBC_colonyPCRprotocol.pdf"><h4>Colony PCR Amplification</h4></a>
−
        </div>
+
<div class="face1"></div>
−
        <div style="height: 0px;" aria-expanded="false" id="collapseOne" class="panel-collapse collapse" role="tabpanel" aria-labelledby="headingOne">
+
  <div class="face2"></div>
−
          <div class="panel-body">
+
</div></a>
−
            <p>
+
 
 +
<a href="#"><div class="hexagon"><a href="https://static.igem.org/mediawiki/2015/3/3b/UBC_compcellsprotocol.pdf"><h4>Inducing Competence</h4></a>
 +
<div class="face1"></div>
 +
  <div class="face2"></div>
 +
</div></a>
 +
 
 +
<a href="#"><div class="hexagon"><a href="https://static.igem.org/mediawiki/2015/1/1d/UBC_electroporationprotocol.pdf"><h4 style="font-size:16px;">Electroporation</h4></a>
 +
<div class="face1"></div>
 +
  <div class="face2"></div>
 +
</div></a>
 +
 
 +
<a href="#"><div class="hexagon"><a href="https://static.igem.org/mediawiki/2015/7/71/UBC_heatshockprotocol.pdf"><h4>Heat Shock</h4></a>
 +
<div class="face1"></div>
 +
  <div class="face2"></div>
 +
</div></a>
 +
 
 +
<a href="#"><div class="hexagon"><a href="https://static.igem.org/mediawiki/2015/b/b6/UBC_conjugationprotocol.pdf"><h4>Conjugation</h4></a>
 +
<div class="face1"></div>
 +
  <div class="face2"></div>
 +
</div></a>
  
−
<div class="button top">
 
−
    <div class="button bottom"></div>
 
 
</div>
 
</div>
−
</p>
+
 
−
<p><a class="btn btn-default" href="https://2015.igem.org/Team:British_Columbia/Notebook/Protocols/PhusionPCR" role="button">View details &raquo;</a></p>
+
<div class="tab-pane" id="b">
−
          </div>
+
 
−
        </div>
+
<a href="#"><div class="hexagon"> <a href="https://static.igem.org/mediawiki/2015/b/b9/Electroporation_IMI_UBC.pdf"><h4>Electroporation</h4></a>
−
      </div>
+
<div class="face1"></div>
−
      <div class="panel panel-default">
+
  <div class="face2"></div>
−
        <div class="panel-heading" role="tab" id="headingTwo">
+
</div></a>
−
          <h4 id="-collapsible-group-item-#2-" class="panel-title">
+
 
−
            <a class="collapsed" data-toggle="collapse" data-parent="#accordion" href="#collapseTwo" aria-expanded="false" aria-controls="collapseTwo">
+
 
−
              <strong>Colony PCR for screening - Taq DNA Polymerase</strong>
+
<a href="#"><div class="hexagon"> <a href="https://static.igem.org/mediawiki/2015/6/61/Gibson_Assembly_UBC.pdf"><h4>Gibson Assembly</h4></a>
−
            </a>
+
<div class="face1"></div>
−
          <a class="anchorjs-link" href="#-collapsible-group-item-#2-"><span class="anchorjs-icon"></span></a></h4>
+
  <div class="face2"></div>
−
        </div>
+
</div></a>
−
        <div style="height: 0px;" aria-expanded="false" id="collapseTwo" class="panel-collapse collapse" role="tabpanel" aria-labelledby="headingTwo">
+
 
−
          <div class="panel-body">
+
<a href="#"><div class="hexagon"> <a href="https://static.igem.org/mediawiki/2015/5/5d/Heat_Shock_IMI_UBC.pdf"><h4>Heat Shock</h4></a>
−
            <p>Colony PCR is commonly used to screen for the presence of a insert in a plasmid during cloning.</p>
+
<div class="face1"></div>
−
<p><a class="btn btn-default" href="https://2015.igem.org/wiki/index.php?title=Team:British_Columbia/Notebook/Protocols/ColonyPCR" role="button">View details &raquo;</a></p>  
+
  <div class="face2"></div>
−
          </div>
+
</div></a>
−
        </div>
+
 
−
      </div>
+
<a href="#"><div class="hexagon"> <a href="https://static.igem.org/mediawiki/2015/a/a5/HPLC_UBC.pdf"><h4>HPLC</h4></a>
−
      <div class="panel panel-default">
+
<div class="face1"></div>
−
        <div class="panel-heading" role="tab" id="headingThree">
+
  <div class="face2"></div>
−
          <h4 id="-collapsible-group-item-#3-" class="panel-title">
+
</div></a>
−
            <a class="collapsed" data-toggle="collapse" data-parent="#accordion" href="#collapseThree" aria-expanded="false" aria-controls="collapseThree">
+
 
−
              <strong>Preparing primers for PCR</strong>
+
 
−
            </a>
+
<a href="#"><div class="hexagon"> <a href="https://static.igem.org/mediawiki/2015/7/7f/Making_Megaprimers_UBC.pdf"><h4>Making Megaprimers</h4></a>
−
          <a class="anchorjs-link" href="#-collapsible-group-item-#3-"><span class="anchorjs-icon"></span></a></h4>
+
<div class="face1"></div>
−
        </div>
+
  <div class="face2"></div>
−
        <div style="height: 0px;" aria-expanded="false" id="collapseThree" class="panel-collapse collapse" role="tabpanel" aria-labelledby="headingThree">
+
</div></a>
−
          <div class="panel-body">
+
 
−
            <p>Before primers can be used for PCR they need to be resuspended and diluted.</p>
+
<a href="#"><div class="hexagon"> <a href="https://static.igem.org/mediawiki/2015/f/fc/Mutagenesis_PCR_UBC.pdf"><h4>Mutagenesis PCR</h4></a>
−
<p><a class="btn btn-default" href="https://2015.igem.org/Team:British_Columbia/Notebook/Protocols/Primers" role="button">View details &raquo;</a></p>
+
<div class="face1"></div>
−
          </div>
+
  <div class="face2"></div>
−
        </div>
+
</div></a>
−
      </div>
+
 
−
    </div>
+
 
 +
<a href="#"><div class="hexagon"> <a href="https://static.igem.org/mediawiki/2015/c/c7/SDS_Page_IMI_UBC.pdf"><h4>SDS Page</h4></a>
 +
<div class="face1"></div>
 +
  <div class="face2"></div>
 +
</div></a>
 +
 
 +
 
 +
<a href="#"><div class="hexagon"> <a href="https://static.igem.org/mediawiki/2015/a/aa/Sonication_UBC.pdf"><h4>Sonication</h4></a>
 +
<div class="face1"></div>
 +
  <div class="face2"></div>
 +
</div></a>
 +
 
 +
<a href="#"><div class="hexagon"> <a href="https://static.igem.org/mediawiki/2015/5/56/Whole_Plasmid_PCR_UBC.pdf"><h4>Whole Plasmid PCR</h4></a>
 +
<div class="face1"></div>
 +
  <div class="face2"></div>
 +
</div></a>
 +
 
 +
 
 +
<a href="#"><div class="hexagon"> <a href="https://static.igem.org/mediawiki/2015/2/2f/Restriction_Digest_UBC.pdf"><h4>Restriction Digest</h4></a>
 +
<div class="face1"></div>
 +
  <div class="face2"></div>
 +
</div></a>
 +
 
 +
 
 
</div>
 
</div>
  
  
 +
<div class="tab-pane" id="c">
  
−
<div class="tab-pane" id="b">Secondo sed ac orci quis tortor imperdiet venenatis. Duis elementum auctor accumsan. Aliquam in felis sit amet augue.</div>
+
<a href="#"><div class="hexagon"> <a href="https://static.igem.org/mediawiki/2015/6/68/GCMS_Protocol_UBC.pdf"><h4>GC/MS Conditions & Preparation</h4></a>
 +
<div class="face1"></div>
 +
  <div class="face2"></div>
 +
</div></a>
  
 +
<a href="#"><div class="hexagon"> <a href="https://static.igem.org/mediawiki/2015/2/2f/Restriction_Digest_UBC.pdf"><h4>Restriction Digest</h4></a>
 +
<div class="face1"></div>
 +
  <div class="face2"></div>
 +
</div></a>
  
−
<div class="tab-pane" id="c">hirdamuno, ipsum dolor sit amet, consectetur adipiscing elit. Duis elementum auctor accumsan. Duis pharetra varius quam sit amet vulputate. Quisque mauris augue, molestie tincidunt condimentum vitae. Thirdamuno, ipsum dolor sit amet, consectetur adipiscing elit. Duis elementum auctor accumsan. Duis pharetra varius quam sit amet vulputate. Quisque mauris augue, molestie tincidunt condimentum vitae. Thirdamuno, ipsum dolor sit amet, consectetur adipiscing elit. Duis elementum auctor accumsan. Duis pharetra varius quam sit amet vulputate. Quisque mauris augue, molestie tincidunt condimentum vitae. Thirdamuno, ipsum dolor sit amet, consectetur adipiscing elit. Duis elementum auctor accumsan. Duis pharetra varius quam sit amet vulputate. Quisque mauris augue, molestie tincidunt condimentum vitae.Thirdamuno, ipsum dolor sit amet, consectetur adipiscing elit. Duis elementum auctor accumsan. Duis pharetra varius quam sit amet vulputate. Quisque mauris augue, molestie tincidunt condimentum vitae. Thirdamuno, ipsum dolor sit amet, consectetur adipiscing elit. Duis elementum auctor accumsan. Duis pharetra varius quam sit amet vulputate. Quisque mauris augue, molestie tincidunt condimentum vitae. Thirdamuno, ipsum dolor sit amet, consectetur adipiscing elit. Duis elementum auctor accumsan. Duis pharetra varius quam sit amet vulputate. Quisque mauris augue, molestie tincidunt condimentum vitae. Thirdamuno, ipsum dolor sit amet, consectetur adipiscing elit. Duis elementum auctor accumsan. Duis pharetra varius quam sit amet vulputate. Quisque mauris augue, molestie tincidunt condimentum vitae. </div>
+
<a href="#"><div class="hexagon"> <a href="https://static.igem.org/mediawiki/2015/1/1e/Liquid_Liquid_Extraction_Protocol_UBC.pdf"><h4>Liquid-Liquid Extraction</h4></a>
 +
<div class="face1"></div>
 +
  <div class="face2"></div>
 +
</div></a>
 +
 
 +
<a href="#"><div class="hexagon"> <a href="https://static.igem.org/mediawiki/2015/b/b8/Preparation_of_Samples_to_run_SDS_UBC.pdf"><h4>Sample Prep for SDS</h4></a>
 +
<div class="face1"></div>
 +
  <div class="face2"></div>
 +
</div></a>
 +
 
 +
<a href="#"><div class="hexagon"> <a href="https://static.igem.org/mediawiki/2015/8/8e/Resting_Cell_Assay_UBC.pdf"><h4>Resting Cell Assay</h4></a>
 +
<div class="face1"></div>
 +
  <div class="face2"></div>
 +
</div></a>
 +
 
 +
<a href="#"><div class="hexagon"> <a href="https://static.igem.org/mediawiki/2015/2/2c/SDS_Gel_Prep_UBC.pdf"><h4>SDS Gel Prep</h4></a>
 +
<div class="face1"></div>
 +
  <div class="face2"></div>
 +
</div></a>
 +
 
 +
<a href="#"><div class="hexagon"> <a href="https://static.igem.org/mediawiki/2015/d/d7/Cell_Lysate_Experiment_of_CCH2_UBC.pdf"><h4>Cell Lysate Experiment</h4></a>
 +
<div class="face1"></div>
 +
  <div class="face2"></div>
 +
</div></a>
 +
 
 +
<a href="#"><div class="hexagon"> <a href="https://static.igem.org/mediawiki/2015/7/7f/Determination_of_CCH2_Rate_UBC.pdf"><h4>CCH2 Rate Experiment</h4></a>
 +
<div class="face1"></div>
 +
  <div class="face2"></div>
 +
</div></a>
 +
 
 +
</div>
 
                 </div>
 
                 </div>
 
             </div>
 
             </div>
Line 98: Line 186:
 
     <!-- /row -->
 
     <!-- /row -->
 
</div>
 
</div>
−
 
+
<div style="clear:both;"></div>
 
   </body>
 
   </body>
 
</html>
 
</html>
 
{{:Team:British Columbia/Template/Fixedfooter}}
 
{{:Team:British Columbia/Template/Fixedfooter}}

Latest revision as of 22:26, 18 September 2015

UBC iGEM 2015

 

Protocols