Difference between revisions of "Team:British Columbia/Notebook/Protocols"

 
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<h1>Protocols: <small>Use the categories on the left to find the protocol you need:</small></h1>
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<div id="one">&nbsp;</div>
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<div id="three"><h3>Protocols</h3></div>
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    <div id="two">&nbsp;</div>
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<h1><small>Select one of the categories below to find the protocol you need:</small></h1>
  
 
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                     <li><a href="#a" data-toggle="tab"><strong>PCR</strong></a></li>
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                     <li style="text-align: left;"><a href="#a" data-toggle="tab"><strong>Genetic Tool Development</strong></a></li>
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                     <li><a href="#b" data-toggle="tab">Two</a></li>
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                     <li style="text-align: center;"><a href="#b" data-toggle="tab"><strong>Imidacloprid Modification</strong></a></li>
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                     <li><a href="#c" data-toggle="tab">Twee</a></li>
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                     <li style="text-align: right;"><a href="#c" data-toggle="tab"><strong>6-CNA Degradation</strong></a></li>
 
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<a href="#"><div class="hexagon"> <a href="https://static.igem.org/mediawiki/2015/1/15/UBC_growinggapicolaprotocol.pdf"><h4>Growing<br /> <i>G. apicola</i></h4></a>
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<a href="#"><div class="hexagon"> <a href="https://static.igem.org/mediawiki/2015/1/1f/UBC_colonyPCRprotocol.pdf"><h4>Colony PCR Amplification</h4></a>
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<a href="#"><div class="hexagon"><a href="https://static.igem.org/mediawiki/2015/3/3b/UBC_compcellsprotocol.pdf"><h4>Inducing Competence</h4></a>
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<a href="#"><div class="hexagon"><a href="https://static.igem.org/mediawiki/2015/1/1d/UBC_electroporationprotocol.pdf"><h4 style="font-size:16px;">Electroporation</h4></a>
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<a href="#"><div class="hexagon"><a href="https://static.igem.org/mediawiki/2015/7/71/UBC_heatshockprotocol.pdf"><h4>Heat Shock</h4></a>
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<a href="#"><div class="hexagon"><a href="https://static.igem.org/mediawiki/2015/b/b6/UBC_conjugationprotocol.pdf"><h4>Conjugation</h4></a>
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              <strong>PCR of inserts for cloning - Phusion DNA polymerase</strong>
 
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            To amplify an insert for cloning, using high-fidelity phusion polymerase.
 
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<p><a class="btn btn-default" href="#" role="button">View details &raquo;</a></p>
 
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              <strong>Colony PCR for screening - Taq DNA Polymerase</strong>
 
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            Colony PCR is commonly used to screen for the presence of a insert in a plasmid during cloning.
 
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              <strong>Preparing primers for PCR</strong>
 
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            Before primers can be used for PCR they need to be resuspended and diluted.
 
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<a href="#"><div class="hexagon"> <a href="https://static.igem.org/mediawiki/2015/b/b9/Electroporation_IMI_UBC.pdf"><h4>Electroporation</h4></a>
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<a href="#"><div class="hexagon"> <a href="https://static.igem.org/mediawiki/2015/6/61/Gibson_Assembly_UBC.pdf"><h4>Gibson Assembly</h4></a>
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<a href="#"><div class="hexagon"> <a href="https://static.igem.org/mediawiki/2015/5/5d/Heat_Shock_IMI_UBC.pdf"><h4>Heat Shock</h4></a>
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<a href="#"><div class="hexagon"> <a href="https://static.igem.org/mediawiki/2015/a/a5/HPLC_UBC.pdf"><h4>HPLC</h4></a>
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<a href="#"><div class="hexagon"> <a href="https://static.igem.org/mediawiki/2015/c/c7/SDS_Page_IMI_UBC.pdf"><h4>SDS Page</h4></a>
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<a href="#"><div class="hexagon"> <a href="https://static.igem.org/mediawiki/2015/a/aa/Sonication_UBC.pdf"><h4>Sonication</h4></a>
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<a href="#"><div class="hexagon"> <a href="https://static.igem.org/mediawiki/2015/6/68/GCMS_Protocol_UBC.pdf"><h4>GC/MS Conditions & Preparation</h4></a>
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Latest revision as of 22:26, 18 September 2015

UBC iGEM 2015

 

Protocols