Difference between revisions of "Team:Stanford-Brown/Parts"
Line 34: | Line 34: | ||
<a href="http://parts.igem.org/Part:BBa_K1692028" class="btn" id="be1" target="_blank"> | <a href="http://parts.igem.org/Part:BBa_K1692028" class="btn" id="be1" target="_blank"> | ||
<h4>Biobrick : BBa_K1692028</h4> | <h4>Biobrick : BBa_K1692028</h4> | ||
− | <p><b>cotz-aeBlue-CipA</b> the first step involved cloning a Bacillus construct in Escherichia coli of a fusion protein sequencing consisting of a spore coat protein, cotZ (building off work done on Sporobeads by the LMU Munich 2012 iGEM team), and a cellulose binding domain (CIPA). Additionally, we decided to add aeBlue, a chromogenic protein, between cotZ and CIPA to be able to see with the naked eye whether Bacillus is in a vegetative or a spore state. </p> | + | <p><b>cotz-aeBlue-CipA</b> the first step involved cloning a </i>Bacillus</i> construct in </i>Escherichia coli</i> of a fusion protein sequencing consisting of a spore coat protein, cotZ (building off work done on Sporobeads by the LMU Munich 2012 iGEM team), and a cellulose binding domain (CIPA). Additionally, we decided to add aeBlue, a chromogenic protein, between cotZ and CIPA to be able to see with the naked eye whether </i>Bacillus</i> is in a vegetative or a spore state. </p> |
</a> | </a> | ||
</div> | </div> | ||
Line 68: | Line 68: | ||
<a href="http://parts.igem.org/Part:BBa_K1692028" class="btn" id="be1" target="_blank"> | <a href="http://parts.igem.org/Part:BBa_K1692028" class="btn" id="be1" target="_blank"> | ||
<h4>Biobrick : BBa_K1692028</h4> | <h4>Biobrick : BBa_K1692028</h4> | ||
− | <p><b>cotz-aeBlue-CipA</b> the first step involved cloning a Bacillus construct in Escherichia coli of a fusion protein sequencing consisting of a spore coat protein, cotZ (building off work done on Sporobeads by the LMU Munich 2012 iGEM team), and a cellulose binding domain (CIPA). Additionally, we decided to add aeBlue, a chromogenic protein, between cotZ and CIPA to be able to see with the naked eye whether Bacillus is in a vegetative or a spore state. </p> | + | <p><b>cotz-aeBlue-CipA</b> the first step involved cloning a </i>Bacillus</i> construct in Escherichia coli of a fusion protein sequencing consisting of a spore coat protein, cotZ (building off work done on Sporobeads by the LMU Munich 2012 iGEM team), and a cellulose binding domain (CIPA). Additionally, we decided to add aeBlue, a chromogenic protein, between cotZ and CIPA to be able to see with the naked eye whether Bacillus is in a vegetative or a spore state. </p> |
</a> | </a> | ||
</div> | </div> |
Revision as of 22:58, 18 September 2015
Our Biobricks So many bricks
See our bricks below!
BioHYDRA BioBricks
Polystyrene Synthesis BioBricks
P(3HB) Synthesis BioBricks
Cellulose Sheets BioBricks