Difference between revisions of "Team:British Columbia/Part Collection"

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<p> Composite parts with multiple degradation genes were designed so that each gene in the construct had a dedicated rbs, promoter, and double terminator. </p>
 
<p> Composite parts with multiple degradation genes were designed so that each gene in the construct had a dedicated rbs, promoter, and double terminator. </p>
  
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Revision as of 00:43, 19 September 2015

UBC iGEM 2015

 

Part Collection

 

Our collection of parts contains genes required for modification of imidacloprid and degradation of 6-chloronicotinic acid (6-CNA). Final parts were assembled in pSB1C3 backbone harboring the chloramphenicol resistance gene and designed to have a LacI repressor, ribosome binding site, pTAC promoter, our degradation genes of interest, and double terminator.

The three composite parts each containing a cytochrome P450 (CYP) were designed to include an N-terminal pelB signal sequence to target expression to the periplasm and a cytochrome P450 reductase (CPR) for functionality of the CYP. The CPR was also made with it’s own designated rbs, promoter, pelB signal sequence, and terminator.

Composite parts with multiple degradation genes were designed so that each gene in the construct had a dedicated rbs, promoter, and double terminator.

3 Column Responsive Layout

1st Content Area

This page demonstrates a 3 column responsive layout, complete with responsive images and jquery slideshow.

2nd Content Area

At full width all three columns will be displayed side by side. As the page is resized the third column will collapse under the first and second. At the smallest screen size all three columns will be stacked on top of one another.

Ut wisi enim ad minim veniam, quis nostrud exerci tation ullamcorper suscipit lobortis nisl ut aliquip ex ea commodo consequat. Duis autem vel eum iriure dolor in hendrerit in vulputate velit esse molestie consequat, vel illum dolore eu feugiat nulla facilisis at vero eros et accumsan et iusto odio dignissim qui blandit praesent luptatum zzril delenit augue duis dolore te feugait nulla facilisi.

Footer

Footer text