Difference between revisions of "Team:Freiburg/Safety"

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{{Freiburg}}
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{{Freiburg/CSS}}
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{{Freiburg/Menubar}}
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{{Team:Freiburg/wiki_content_start_bubble}}
 
<html>
 
<html>
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<h2>Safety in iGEM</h2>
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<style>
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/*========= BEGIN: style for navigation bar ==========*/
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#notebook {
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    background: url(https://static.igem.org/mediawiki/2015/c/cd/Freiburg_icon_notebook_active_yellow.png) no-repeat;
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}
  
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<p>Please visit <a href="https://2015.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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#notebook a {
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    color: #ecdc18;
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}
  
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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#runningchip {
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    left: 85.5%;
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}
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/*========= END: style for navigation bar ==========*/
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</style>
  
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<script type="text/javascript">
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//===================BEGIN:Amazing Bubble Sidebar==========================
  
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<h4>Safe Project Design</h4>
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$(document).ready(function(){
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  // CHANGE THE FOLLOWING ATTRIBUTES //
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  var href_text1='https://2015.igem.org/Team:Freiburg/Notebook',
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  // Text2 needs no href as it is the actual page //
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  img_url='https://static.igem.org/mediawiki/2015/9/99/Freibur_icon_notebook_white_03.png',
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  href_text3='https://2015.igem.org/Team:Freiburg/Wikimigration',
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  // Text1 needs no text as it is a pic //
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  text2='Safety';
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  // Text3 needs no text as its always 'next' //
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  // HOLD ON CHANGING THINGS --JABBERWOCK  //
  
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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  $('#bubble1').attr('href',href_text1);
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  $('#bubble1_img').attr('src', img_url);
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  $('#bubble3').attr('href',href_text3);
  
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  $('#bubble2').text(text2);
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});
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//===================END:Amazing Bubble Sidebar==========================
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</script>
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<div class="content_box">
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<h1>Safety</h1>
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</br>
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<h2>Safety Instructions</h2>
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<p>Every member of our team received an instruction for general lab safety from Dr. Nicole Gensch, the official safety instructor of the BIOSS (Centre for Biological Signalling Studies). Furthermore, our team received a special training for correct handling and disposal of liquid nitrogen from Dr. Johannes Kaiser, the executive director of the BIOSS. As we had to perform some of our measurements in a specialized lab at the ZBSA (Center for Biological Systems Analysis) we also received a safety instruction for their spatiality from Dr. Günter Roth.</p>
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<h2>Lab Safety</h2>
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<p> In order to avoid any harmful influences to one of our precious team members all lab work was performed in accordance to the German regulations for lab safety (GUV-R 120, TRBA 100). This includes, but is not limited, to the following examples:</p>
 
<ul>
 
<ul>
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<li>Choosing a non-pathogenic chassis</li>
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<li>Wearing personal protection equipment like lab coats, safety goggles and rubber gloves if needed</li>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<li>Consuming any food or drinks in the lab area is prohibited</li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li>Smoking in the lab area is prohibited</li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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<li>Autoclaving all waste that came into contact with biological material is mandatory</li>
 
</ul>
 
</ul>
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<br>
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<div class="image_box center">
  
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<h4>Safe Lab Work</h4>
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<div class="thumb2 trien" style="width:310px">
  
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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                                <div class="thumbinner">
  
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<h4>Safe Shipment</h4>
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                                <a href="https://static.igem.org/mediawiki/2015/b/b8/Freiburg_safety.jpeg" class="lightbox_trigger">
  
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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                                  <img src="https://static.igem.org/mediawiki/2015/b/b8/Freiburg_safety.jpeg" width="300px">  
  
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                                                <div class="thumbcaption">
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                                                </a>
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                                              </div>
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                                              </div>
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                                              </div>
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                                              </div>
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<h2>Minimizing Risks</h2>
 +
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<p>To further reduce the possibility of a potential dangerous situation happening to one of our team members, we designed our project to be as safe as possible.
 +
</p>
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<h3>Dangerous Chemicals</h3>
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<p>We tried to avoid using dangerous chemicals whenever possible. We completely prohibited the use of ethidium bromide for example and used Midori Green as safer alternative to dye our DNA gels. We also restricted all use of this chemical to a small and clearly labelled area of the lab.</p>
 +
 +
<h3>Biological Hazards</h3>
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<p> Because of the nature of our project, working with antigens derived from pathogenic organisms was unavoidable. To nevertheless ensure safe working conditions, we decided to use only proteins that have no virulent or toxic potential. Furthermore, we tried not to use whole proteins but only immunogenic epitopes. Like this, the detection of antibodies was still maintained while at the same time not using whole functional proteins.</br>
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To further reduce the risk of biological hazards we only used harmless <a class="wikilink1" href="https://2015.igem.org/Team:Freiburg/Project/Coli_Strains" title="strains">lab strains of <i>E. coli</i></a> (risk group 1) for cloning and expression of all of our needed plasmids and proteins.
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</p>
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<h3>Working Structure</h3>
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<p> By using a seminar room of the University of Freiburg for relaxing, meetings and all kind of organisational work we were able to reserve the lab for actual lab work. This clear cut between lab and office work helps to prevent unnecessary complications and contaminations that might occur in a hectic and overcrowded lab.
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</p>
  
 
</div>
 
</div>
 
</html>
 
</html>
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{{Freiburg/wiki_content_end}}

Latest revision as of 01:27, 19 September 2015

""

Safety


Safety Instructions

Every member of our team received an instruction for general lab safety from Dr. Nicole Gensch, the official safety instructor of the BIOSS (Centre for Biological Signalling Studies). Furthermore, our team received a special training for correct handling and disposal of liquid nitrogen from Dr. Johannes Kaiser, the executive director of the BIOSS. As we had to perform some of our measurements in a specialized lab at the ZBSA (Center for Biological Systems Analysis) we also received a safety instruction for their spatiality from Dr. Günter Roth.

Lab Safety

In order to avoid any harmful influences to one of our precious team members all lab work was performed in accordance to the German regulations for lab safety (GUV-R 120, TRBA 100). This includes, but is not limited, to the following examples:

  • Wearing personal protection equipment like lab coats, safety goggles and rubber gloves if needed
  • Consuming any food or drinks in the lab area is prohibited
  • Smoking in the lab area is prohibited
  • Autoclaving all waste that came into contact with biological material is mandatory

Minimizing Risks

To further reduce the possibility of a potential dangerous situation happening to one of our team members, we designed our project to be as safe as possible.

Dangerous Chemicals

We tried to avoid using dangerous chemicals whenever possible. We completely prohibited the use of ethidium bromide for example and used Midori Green as safer alternative to dye our DNA gels. We also restricted all use of this chemical to a small and clearly labelled area of the lab.

Biological Hazards

Because of the nature of our project, working with antigens derived from pathogenic organisms was unavoidable. To nevertheless ensure safe working conditions, we decided to use only proteins that have no virulent or toxic potential. Furthermore, we tried not to use whole proteins but only immunogenic epitopes. Like this, the detection of antibodies was still maintained while at the same time not using whole functional proteins.
To further reduce the risk of biological hazards we only used harmless lab strains of E. coli (risk group 1) for cloning and expression of all of our needed plasmids and proteins.

Working Structure

By using a seminar room of the University of Freiburg for relaxing, meetings and all kind of organisational work we were able to reserve the lab for actual lab work. This clear cut between lab and office work helps to prevent unnecessary complications and contaminations that might occur in a hectic and overcrowded lab.