Difference between revisions of "Team:British Columbia/Part Collection"
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| + | Cch2 is a novel chlorohydrolase that was identified in a soil bacterium, Bradyrhizobiaceae strain SG-6C, that is capable of dechlorinating 6-chloronicotinic acid (6-CNA) to 6-hydroxynicotinic acid (6-HNA). 6-CNA has been found to accumulate in soils and plant material following application of imidacloprid. Due to the associated toxicity of 6-CNA to bees, demonstrating that this part can be used to degrade 6-CNA to 6-HNA, a less toxic metabolite, was a big accomplishment (see data here). rem ipsum dolor sit amet, consectetur adipiscing elit. Sed posuere eget nulla a pharetra. Suspendisse ac venenatis odio. Sed blandit posuere erat a posuere. Suspendisse odio erat, elementum sodales ante vel, malesuada rutrum turpis.</p> | ||
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Revision as of 01:29, 19 September 2015
Part Collection
Parts Collection!
Our collection of parts contains genes required for modification of imidacloprid and degradation of 6-chloronicotinic acid (6-CNA). Final parts were assembled in pSB1C3 backbone harboring the chloramphenicol resistance gene and designed to have a LacI repressor, ribosome binding site, pTAC promoter, our degradation genes of interest, and double terminator.
The three composite parts each containing a cytochrome P450 (CYP) were designed to include an N-terminal pelB signal sequence to target expression to the periplasm and a cytochrome P450 reductase (CPR) for functionality of the CYP. The CPR was also made with it’s own designated rbs, promoter, pelB signal sequence, and terminator.
Composite parts with multiple degradation genes were designed so that each gene in the construct had a dedicated rbs, promoter, and double terminator.
Favourite Parts:
LoBBa_K1813026 Cch2 is a novel chlorohydrolase that was identified in a soil bacterium, Bradyrhizobiaceae strain SG-6C, that is capable of dechlorinating 6-chloronicotinic acid (6-CNA) to 6-hydroxynicotinic acid (6-HNA). 6-CNA has been found to accumulate in soils and plant material following application of imidacloprid. Due to the associated toxicity of 6-CNA to bees, demonstrating that this part can be used to degrade 6-CNA to 6-HNA, a less toxic metabolite, was a big accomplishment (see data here). rem ipsum dolor sit amet, consectetur adipiscing elit. Sed posuere eget nulla a pharetra. Suspendisse ac venenatis odio. Sed blandit posuere erat a posuere. Suspendisse odio erat, elementum sodales ante vel, malesuada rutrum turpis.
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Sed posuere eget nulla a pharetra. Suspendisse ac venenatis odio. Sed blandit posuere erat a posuere. Suspendisse odio erat, elementum sodales ante vel, malesuada rutrum turpis.
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Sed posuere eget nulla a pharetra. Suspendisse ac venenatis odio. Sed blandit posuere erat a posuere. Suspendisse odio erat, elementum sodales ante vel, malesuada rutrum turpis.
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Sed posuere eget nulla a pharetra. Suspendisse ac venenatis odio. Sed blandit posuere erat a posuere. Suspendisse odio erat, elementum sodales ante vel, malesuada rutrum turpis.
UBC iGEM 2015