Difference between revisions of "Team:METU Turkey/Experiments"
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<a class="one" href="https://2015.igem.org/Team:METU_Turkey/Safety">Safety</a> | <a class="one" href="https://2015.igem.org/Team:METU_Turkey/Safety">Safety</a> | ||
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<div class="highlightBox"> | <div class="highlightBox"> | ||
<h2 align=center>Experiments/Documentation of the development of our project</h2> | <h2 align=center>Experiments/Documentation of the development of our project</h2> | ||
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<pre> | <pre> | ||
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Competent cells were prepared | Competent cells were prepared | ||
7 strains | 7 strains | ||
− | Transformation was done at night for control purposes, 5 strains were negative (amp plate 4 min 4000 rpm) | + | Transformation was done at night for control purposes, |
+ | 5 strains were negative (amp plate 4 min 4000 rpm) | ||
July 17th, Friday | July 17th, Friday | ||
Kitten gene was extracted | Kitten gene was extracted | ||
5 strains | 5 strains | ||
− | Transformation was done for control purposes. This was so because 5 strains were negative in the previous one. | + | Transformation was done for control purposes. |
+ | This was so because 5 strains were negative in the previous one. | ||
Kumomax enzyme was planted from agar plate to agar plate. | Kumomax enzyme was planted from agar plate to agar plate. | ||
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Plasmid isolation was done for 101, 106, 117 and GFP | Plasmid isolation was done for 101, 106, 117 and GFP | ||
Restriction was done | Restriction was done | ||
− | Gel electrophoresis was done for the cut 101, 106, 117 and GFP, the results were negative | + | Gel electrophoresis was done for the cut 101, 106, 117 and GFP, |
+ | the results were negative | ||
101, 106, 117 and GFP were again planted in LB from agar | 101, 106, 117 and GFP were again planted in LB from agar | ||
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Plasmid isolation was done for 101, 106, 117 and GFP | Plasmid isolation was done for 101, 106, 117 and GFP | ||
Restriction was done for 101, 106, 117 and GFP | Restriction was done for 101, 106, 117 and GFP | ||
− | Gel electrophoresis was done for the cut 101, 106, 117 and GFP, the results were positive only for the promoters, 101, 106, and 117 | + | Gel electrophoresis was done for the cut 101, 106, 117 and GFP, |
+ | the results were positive only for the promoters, 101, 106, and 117 | ||
Gel extraction was done for promoters | Gel extraction was done for promoters | ||
Nanodrop results were low | Nanodrop results were low | ||
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Optimization was done for restriction | Optimization was done for restriction | ||
− | + | <img hspace="80px" src="https://static.igem.org/mediawiki/2015/2/2b/4_sayfa_elif_tablo.PNG" width="400px"> | |
− | <img hspace=" | + | |
− | + | ||
− | The electrophoresis results were acquired for set 2 and set 2, set 1 did not produce results | + | The electrophoresis results were acquired for set 2 and set 2, set 1 |
+ | did not produce results | ||
Gel extraction was done for the 4 bands that yielded positive results | Gel extraction was done for the 4 bands that yielded positive results | ||
Nanodrop results were low | Nanodrop results were low | ||
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August 7th, Friday | August 7th, Friday | ||
− | The transformations done for promoter-GFP and PGapZ-alpha-kumomaz were negative | + | The transformations done for promoter-GFP and PGapZ-alpha- |
+ | kumomaz were negative | ||
400 mL Amp agar medium was prepared, put into plates | 400 mL Amp agar medium was prepared, put into plates | ||
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August 11th, Tuesday | August 11th, Tuesday | ||
− | The streaked plated 101, 106,117 and GFP were planted in LB (8 tubes in total) | + | The streaked plated 101, 106,117 and GFP were planted in |
+ | LB (8 tubes in total) | ||
LB was prepared (400 mL, 5 g salt) | LB was prepared (400 mL, 5 g salt) | ||
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Plasmid isolation was done for 101, 106, 117 and GFP | Plasmid isolation was done for 101, 106, 117 and GFP | ||
Restriction was done for GFP | Restriction was done for GFP | ||
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+ | <img hspace="80px" src="https://static.igem.org/mediawiki/2015/3/3a/7_son_sayfa_elif_tablo.PNG" width="400px"> | ||
</pre> | </pre> | ||
</div></br> | </div></br> |
Latest revision as of 13:29, 4 October 2015
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