Difference between revisions of "Team:KU Leuven/Symposium"

(Created page with "{{KU_Leuven}} {{KU_Leuven/css}} {{KU_Leuven/research/idea/css}} {{KU_Leuven/Lightbox/css}} {{KU_Leuven/EasySwitch/CSS}} <html> <style> #research{ background-color: transpa...")
 
 
(320 intermediate revisions by 8 users not shown)
Line 1: Line 1:
 
{{KU_Leuven}}
 
{{KU_Leuven}}
 
{{KU_Leuven/css}}
 
{{KU_Leuven/css}}
−
{{KU_Leuven/research/idea/css}}
 
 
{{KU_Leuven/Lightbox/css}}
 
{{KU_Leuven/Lightbox/css}}
−
{{KU_Leuven/EasySwitch/CSS}}
 
  
 
<html>
 
<html>
  
−
<style>
+
    <script>
−
#research{  
+
        $(document).onload(function () {
−
    background-color: transparent;
+
            $(".main-navm").hide();
−
    border-style: solid;
+
        }
−
     border: 0px solid transparent;
+
     </script>
−
    border-bottom: 5px solid #8b7a57;   
+
−
}
+
  
−
#centernav:hover #research {  /* this is active when your mouse moves is over the item */
+
     <style>
−
     border-style: solid;
+
−
    border-bottom: 0px solid transparent;
+
−
}
+
  
−
#content {
+
        #outreach {
−
    background-color:transparent;
+
            background-color: transparent;
−
}
+
            border-style: solid;
 +
            border: 0 solid transparent;
 +
            border-bottom: 5px solid #8b7a57;
 +
        }
 +
        #centernav:hover #outreach{
 +
            /* this is active when your mouse moves is over the item */
 +
            border: 0 solid transparent;
 +
            border-bottom: 0 solid transparent;
 +
        }
 +
        .main-navm:hover #outreach {
 +
            /* this is active when your mouse moves is over the item */
 +
            border: 0 solid transparent;
 +
            border-right: 0 solid transparent;
 +
        }
 +
        @media screen and (max-width: 1000px) {#outreach {
 +
                border-bottom: 5px solid transparent;
 +
                border-right: 5px solid #8b7a57;
 +
            }
 +
        }
 +
        #content {
 +
            background-color: transparent;
 +
        }
 +
    </style>
  
−
</style>
+
    <style>
−
+
        h3 {
−
<link rel="stylesheet" type="text/css"  
+
            font-size: 2.3em;
−
href="https://2015.igem.org/Template:KU_Leuven/Lightbox/CSS?action=raw&ctype=text/css" />
+
        }
 +
        h2 {
 +
            font-size: 2.6em;
 +
            line-height: 120%;
 +
            text-allign="center";
 +
            text-transform: none;
 +
        }
 +
        b1 {
 +
            font-size: 0.85em;
 +
        }
 +
        p2 {
 +
            margin-top: 10px;
 +
            margin-bottom: 0;
 +
            margin-left: 10px;
 +
            margin-right: 10px;
 +
            text-allign="left";
 +
        }
 +
        b {
 +
            font-size: 1em;
 +
        }
 +
        #content {
 +
            background-color: transparent;
 +
        }
 +
    </style>
 +
 
 +
    <link
 +
        href="https://2015.igem.org/Template:KU_Leuven/Lightbox/CSS?action=raw&ctype=text/css"
 +
        rel="stylesheet" type="text/css"/>
 
</head>
 
</head>
−
<script type="text/javascript" src="http://yourjavascript.com/11518521655/lightbox.js"></script>
+
<script src="http://yourjavascript.com/11518521655/lightbox.js"
 +
    type="text/javascript"></script>
  
 
<body>
 
<body>
  
−
<div class="summaryheader">
+
    <div class="summaryheader">
−
  <div class="summaryimg">
+
        <div class="summaryimg">
−
  <img src="https://static.igem.org/mediawiki/2015/b/b7/KU_Leuven_Researchbanner.jpg" width="100%">
+
            <img src="https://static.igem.org/mediawiki/2015/4/4d/KU_Leuven_Banner_Blauw.jpg"
−
  <div class="head">
+
                width="100%">
−
    <h2> Symposium </h2>
+
            <div class="head">
−
  </div>
+
                <h2>
−
</div>
+
                    Symposium
−
</div>
+
                </h2>
 +
            </div>
 +
        </div>
 +
    </div>
  
 +
    <!------------------------------------------------------Begin Content------------------------------------------->
 +
    <div class="summarytext1">
 +
          <div class="part">
 +
            <h2>
 +
                Event summary
 +
            </h2>
 +
            <p>
 +
                On September the 7th, 2015 we organized the <b>KU Leuven iGEM 2015 Symposium on Synthetic Biology, Cell Systems and Ethics in Biochemistry</b>. Hosted guests included 4 neighbouring iGEM teams, academic staff, students, sponsors, and iGEM supporters from 6 different European countries. During this full-day event, the participants had a chance to attend the lectures by home speakers as well as by invited international keynote speakers from both the academia and the industry. The iGEM teams presented their research, and probably the most awaited part - the debate on ethics in biochemistry with panel of well selected experts lead by an experienced professional moderator - took place in the afternoon. To fulfil all the needs, goodie-bags, drinks, lunch, and dinner were provided to our guests. The day finished with a sightseeing walk around Leuven centre. Participation was free of charge. We hosted almost a hundred guests and received a very positive feedback. <br/></p>
 +
                <br/>
 +
                <br/>
 +
                <div class="center">
 +
                  <div class="quote">
 +
                    <h2>
 +
                      Evaluation form quotes
 +
                    </h2>
 +
                    <p>
 +
                    "Very professionally organized. Very well run. Great speakers - diverse agenda." <br/>
 +
                    "The lectures were super interesting!" <br/>
 +
                    "Great symposium, I loved it!!" <br/>
 +
                    "Amazing keynote speakers and a fantastic moderator. Everything was perfectly organized.” <br/>
 +
                    "Food was very good!" <br/>
 +
                    "It was the best symposium I have ever attended!!! Outstanding organization!!!" <br/>
 +
                    "Very good symposium. The keynote speakers were marvellous, the debate was interesting, with panel members who were very well selected!" <br/>
 +
                    <p>
 +
                  </div>
 +
                </div>
 +
<div class="whiterow"></div>
 +
            <p>
 +
                Home speaker <b>Vera van Noort</b> talked about lessons from systems biology of a
 +
                minimal organism for synthetic biology. In the EMBL, where she worked, they
 +
                wanted to completely describe one organism, namely <i>Mycoplasma
 +
                pneumoniae</i>. For complete understanding, three different levels were studied: <br/>
 +
                The metabolism, transcription (regulation) and protein complexes. The take-home
 +
                messages for synthetic biology in short: transcription regulation is more than
 +
                operons and transcription factors; enzymes can catalyse multiple reactions and
 +
                are organized in multi-subunit complexes; proteins can be part of multiple
 +
                complexes and can be regulated by post-translational modifications and small
 +
                peptides are essential. <br/><br/>
 +
                <b>Victor Dillard</b>, the first keynote speaker, is the
 +
                founder of Desktop Genetics. The company is building software for biologists,
 +
                with a focus on synthetic biology. The software helps to improve the efficiency
 +
                and to lower the costs. He explained that genome editing remains hard and became
 +
                a design and software challenge, and not purely biological challenge. This is
 +
                because genome editing needs to be accurate, precise, effective and rapid.<br/><br/>
 +
                <b>Sebastian Maerkl</b>, the second keynote speaker, discussed the topic cell-free
 +
                synthetic biology. He explained that microfluidics with cell-free lysate can be
 +
                used for rapid prototyping of biological systems. One of the advantages is that
 +
                it has defined and controllable reaction conditions. <i>In vitro</i> prototyping is
 +
                used to speed up research in synthetic biology. The pipeline of cell-free
 +
                synthetic biology: design a biological circuit, build the circuit, test parts
 +
                and circuits, characterize working circuits, clone and implement <i>in vivo</i>.<br/><br/>
 +
                The second home speaker, <b>Yves Peeters</b>, gave a talk about directed evolution of
 +
                polymerases using synthetic biology methods. The research of Yves Peeters is
 +
                part of the research domain Xenobiology, creating alternative life, one of the
 +
                approaches of synthetic biology. XNA, also called orthogonal DNA, is designed by
 +
                several labs using different strategies. Making organisms with XNA will be an
 +
                ultimate biosafety tool for synthetic biology. Before being able to have a
 +
                liveable organism that uses XNA, there is a need for polymerases recognizing the
 +
                specific XNA. To create the wanted polymerases, Yves uses directed evolution,
 +
                including mutagenesis, screening, amplification and iteration of the most active
 +
                enzymes.<br/>
 +
            </p>
 +
            <br/>
 +
            <br/>
 +
            <div class="center">
 +
              <div id="image1">
 +
              <a class="example-image-set" href="https://static.igem.org/mediawiki/2015/b/b6/KU_Leuven_symp_cut.JPG"
 +
                data-lightbox="example-set" data-title="Photo of the participating speakers and
 +
                iGEM teams at the symposium."><img class="example-image"
 +
                src="https://static.igem.org/mediawiki/2015/b/b6/KU_Leuven_symp_cut.JPG" alt="Photo of the participating speakers and
 +
                iGEM teams at the symposium. " width="49%" height="49%"></a>
  
−
<!------------------------------------------------------Begin Content--------------------------------------------------------->
+
              <a class="example-image-set" href="https://static.igem.org/mediawiki/2015/6/65/KU_Leuven_sym1.jpg"
 +
                data-lightbox="example-set"> </a>
 +
              <a class="example-image-set" href="https://static.igem.org/mediawiki/2015/1/1d/KU_Leuven_sym2.jpg"
 +
                data-lightbox="example-set"> </a>
 +
              <a class="example-image-set" href="https://static.igem.org/mediawiki/2015/0/05/KU_Leuven_sym3.jpg"
 +
                data-lightbox="example-set"> </a>
 +
              <a class="example-image-set" href="https://static.igem.org/mediawiki/2015/b/b6/KU_Leuven_sym6.jpg"
 +
                data-lightbox="example-set"> </a>
 +
              <a class="example-image-set" href="https://static.igem.org/mediawiki/2015/e/ec/KU_Leuven_sym7.jpg"
 +
                data-lightbox="example-set"> </a>
 +
              <a class="example-image-set" href="https://static.igem.org/mediawiki/2015/7/76/KU_Leuven_sym9.jpg"
 +
                data-lightbox="example-set"> </a>
 +
              <a class="example-image-set" href="https://static.igem.org/mediawiki/2015/b/b8/KU_Leuven_sym13.jpg"
 +
                data-lightbox="example-set"> </a>
 +
              <a class="example-image-set" href="https://static.igem.org/mediawiki/2015/3/3f/KU_Leuven_sym15.jpg"
 +
                data-lightbox="example-set"> </a>
 +
              <a class="example-image-set" href="https://static.igem.org/mediawiki/2015/1/11/KU_Leuven_sym16.jpg"
 +
                data-lightbox="example-set"> </a>
 +
              <a class="example-image-set" href="https://static.igem.org/mediawiki/2015/3/3b/KU_Leuven_sym19.jpg"
 +
                data-lightbox="example-set"> </a>
 +
              <a class="example-image-set" href="https://static.igem.org/mediawiki/2015/1/13/KU_Leuven_sym22.jpg"
 +
                data-lightbox="example-set"> </a>
 +
              <a class="example-image-set" href="https://static.igem.org/mediawiki/2015/f/fe/KU_Leuven_sym23.jpg"
 +
                data-lightbox="example-set"> </a>
 +
              <a class="example-image-set" href="https://static.igem.org/mediawiki/2015/d/dc/KU_Leuven_sym24.jpg"
 +
                data-lightbox="example-set"> </a>
 +
              <a class="example-image-set" href="https://static.igem.org/mediawiki/2015/4/4d/KU_Leuven_sym25.jpg"
 +
                data-lightbox="example-set"> </a>
 +
              <a class="example-image-set" href="https://static.igem.org/mediawiki/2015/c/ce/KU_Leuven_sym26.jpg"
 +
                data-lightbox="example-set"> </a>
 +
              <a class="example-image-set" href="https://static.igem.org/mediawiki/2015/c/c1/KU_Leuven_sym27.jpg"
 +
                data-lightbox="example-set"> </a>
 +
              <a class="example-image-set" href="https://static.igem.org/mediawiki/2015/b/b3/KU_Leuven_sym28.jpg"
 +
                data-lightbox="example-set"> </a>
 +
              <a class="example-image-set" href="https://static.igem.org/mediawiki/2015/5/58/KU_Leuven_sym29.jpg"
 +
                data-lightbox="example-set"> </a>
 +
              <a class="example-image-set" href="https://static.igem.org/mediawiki/2015/b/b3/KU_Leuven_sym28.jpg"
 +
                data-lightbox="example-set"> </a>
 +
              <a class="example-image-set" href="https://static.igem.org/mediawiki/2015/7/77/KU_Leuven_sym32.jpg"
 +
                data-lightbox="example-set"> </a>
 +
              <a class="example-image-set" href="https://static.igem.org/mediawiki/2015/5/50/KU_Leuven_sym33.jpg"
 +
                data-lightbox="example-set"> </a>
 +
              <a class="example-image-set" href="https://static.igem.org/mediawiki/2015/f/f1/KU_Leuven_sym34.jpg"
 +
                data-lightbox="example-set"> </a>
 +
              <a class="example-image-set" href="https://static.igem.org/mediawiki/2015/d/da/KU_Leuven_sym35.jpg"
 +
                data-lightbox="example-set"> </a>
 +
              <a class="example-image-set" href="https://static.igem.org/mediawiki/2015/8/80/KU_Leuven_sym36.jpg"
 +
                data-lightbox="example-set"> </a>
 +
              <a class="example-image-set" href="https://static.igem.org/mediawiki/2015/c/cb/KU_Leuven_sym37.jpg"
 +
                data-lightbox="example-set"> </a>
 +
              <a class="example-image-set" href="https://static.igem.org/mediawiki/2015/2/2b/KU_Leuven_sym38.jpg"
 +
                data-lightbox="example-set"> </a>
 +
              <a class="example-image-set" href="https://static.igem.org/mediawiki/2015/5/59/KU_Leuven_sym39.jpg"
 +
                data-lightbox="example-set"> </a>
 +
              <a class="example-image-set" href="https://static.igem.org/mediawiki/2015/5/54/KU_Leuven_sym40.jpg"
 +
                data-lightbox="example-set"> </a>
 +
              <a class="example-image-set" href="https://static.igem.org/mediawiki/2015/9/98/KU_Leuven_sym41.jpg"
 +
                data-lightbox="example-set"> </a>
 +
              <a class="example-image-set" href="https://static.igem.org/mediawiki/2015/d/d0/KU_Leuven_sym42.jpg"
 +
                data-lightbox="example-set"> </a>
 +
              <a class="example-image-set" href="https://static.igem.org/mediawiki/2015/a/ab/KU_Leuven_sym44.jpg"
 +
                data-lightbox="example-set"> </a>
 +
              <a class="example-image-set" href="https://static.igem.org/mediawiki/2015/d/d1/KU_Leuven_sym45.jpg"
 +
                data-lightbox="example-set"> </a>
 +
              <a class="example-image-set" href="https://static.igem.org/mediawiki/2015/b/b1/KU_Leuven_sym47.jpg"
 +
                data-lightbox="example-set"> </a> 
 +
              <a class="example-image-set" href="https://static.igem.org/mediawiki/2015/a/af/KU_Leuven_sym48.jpg"
 +
                data-lightbox="example-set"> </a> 
 +
              <a class="example-image-set" href="https://static.igem.org/mediawiki/2015/3/39/KU_Leuven_sym49.jpg"
 +
                data-lightbox="example-set"> </a> 
 +
              <a class="example-image-set" href="https://static.igem.org/mediawiki/2015/b/b3/KU_Leuven_sym50.jpg"
 +
                data-lightbox="example-set"> </a> 
 +
                <h4><div id=figure2></div> Photo of the
 +
                participating speakers and iGEM teams at the symposium. Click and  on image and use arrow buttons
 +
                to browse trough more pictures. </h4>
 +
              </div>
 +
            </div>
 +
          </div>
 +
        </div>
 +
 
 +
  <div class="part">
 +
        <h3 style="font-size:2.7em">PARTICIPATING iGEM TEAMS</h3><br>
 +
        <div>
 +
            <div class="linewrapper">
 +
                <div align="left" class="teamswrapper" id="left" name="KUL"
 +
                    style="cursor:pointer;">
 +
                    <a href="https://2015.igem.org/Team:KU_Leuven">
 +
                        <img height=100px src="https://static.igem.org/mediawiki/2015/7/76/KUL_SYM_logo.png"
 +
                            width=200px>
 +
                    </a>
 +
                </div>
 +
 
 +
                <div class="teamswrapper" id="middle" name="AMS" style="cursor:pointer;">
 +
                    <a href="https://2015.igem.org/Team:Amsterdam">
 +
                        <img height=120px
 +
                            src="https://static.igem.org/mediawiki/2015/8/81/KUL_Amsterdam_iGEM_logo_transparant.png"
 +
                            width=200px >
 +
                    </a>
 +
                </div>
 +
 
 +
                <div align="right" class="teamswrapper" id="right" name="PAR"
 +
                    style="cursor:pointer;">
 +
                    <a href="https://2015.igem.org/Team:Paris_Saclay">
 +
                        <img height=120px
 +
                            src="https://static.igem.org/mediawiki/2015/a/ab/KUL_logo_Paris_Saclay_logo_transparant.png
 +
"
 +
                            width=200px>
 +
                    </a>
 +
                </div>
 +
            </div>
 +
 
 +
            <div class="linewrapper">
 +
                <div align="left" class="teamswrapper" id="left" name="EIN"
 +
                    style="cursor:pointer;">
 +
                    <a href="https://2015.igem.org/Team:TU_Eindhoven">
 +
                        <img height=120px src="https://static.igem.org/mediawiki/2015/0/06/Logo_website.png"
 +
                            width=200px>
 +
                    </a>
 +
                </div>
 +
 
 +
                <div class="teamswrapper" id="middle" name="BONN" style="cursor:pointer;">
 +
                    <a href="http://2015.igembonn.com">
 +
                        <img height=120px src="https://static.igem.org/mediawiki/2015/f/fb/KUL_Bonn.jpg"
 +
                            width=200px>
 +
                    </a>
 +
                </div>
 +
            </div>
 +
        </div>
 +
    </div>   
 +
   
 +
    <div class="summarytext1">
 +
        <div class="part">
 +
 
 +
            <h3 style="font-size:2.7em">Speakers</h3><br>
 +
 
 +
            <p style="margin-bottom:20px">
 +
                <img ; align="left" height=225px
 +
                    src="https://static.igem.org/mediawiki/2015/f/f8/KU_Leuven_Speaker_SM.jpg"
 +
                    style="margin: 0px 20px 0px 0px;border:3px solid black" width="150px">
 +
                <u>
 +
                    <b>Sebastian Maerkl</b>, École Polytechnique Fédérale de Lausanne, The
 +
                    Laboratory of Biological Network Characterization (LBNC)</u>
 +
                <br>
 +
                Sebastian Maerkl's lab conducts research at the interface of engineering and
 +
                biology and is active in the areas of systems biology, synthetic biology and
 +
                molecular diagnostics. They are driven by the desire to learn how to rationally
 +
                design and engineer biological systems. Sebastian Maerkl’s research aims to
 +
                develop new microfluidic technologies and apply them to solve biological
 +
                problems. His rare expertise allows him to combine the design of new tools with
 +
                advanced research in biology. Sebastian Maerkl is internationally recognized for
 +
                his many outstanding contributions. Particularly in combining synthetic biology
 +
                and computational systems with microfluidics, he demonstrated that the
 +
                expression of genes in vivo can be provided based on the binding energy profiles
 +
                in vitro. His studies will focus on five areas: the bioengineering of
 +
                biosystems, the engineering of transcriptional regulatory networks, the
 +
                engineering of genes and genomes, the engineering of biological systems de novo
 +
                and the development of a new generation of diagnostic devices.
 +
            </p>
 +
        </div>
 +
    </div>
 +
    <br>
 +
    <br>
 +
    <br>
 +
    <br>
 +
    <div class="summarytext1">
 +
        <div class="part">
 +
            <p style="margin-bottom:20px">
 +
                <img ; align="right" height="210px"
 +
                    src="https://static.igem.org/mediawiki/2015/archive/7/7f/20150820114120%21KUL_SYM_VD.png"
 +
                    style="margin: 0px 0px 0px 20px;border:3px solid black" width="180px">
 +
                <u>
 +
                    <b>Victor Dillard</b>, Chief Operating Officer & Founder, Desktop Genetics</u>
 +
                <br>
 +
                Victor obtained his masters in chemical engineering with honours at Imperial
 +
                College London before completing a specialist biotechnology and business masters
 +
                with distinction at the University of Cambridge. Since graduating, Victor
 +
                founded Desktop Genetics with a vision to change modern biotech R&D and enable
 +
                rapid and accurate end-to-end genome engineering experiments through their
 +
                proprietary software platform. Within two years of founding Desktop Genetics,
 +
                Victor has raised over $600,000 of private equity and grant financing, and
 +
                delivered over $400,000 of revenue. Today, Victor heads the company's business
 +
                development and operations and is leading the product and technology expansion
 +
                into CRISPR and genome editing.
 +
            </p>
 +
        </div>
 +
    </div>
 +
    <div class="whitespace"></div>
 +
    <br>
 +
    <br>
 +
    <br>
 +
    <br>
 +
    <div class="summarytext1">
 +
        <div class="part">
 +
            <p>
 +
                <img ; align="left" height=200px
 +
                    src="https://static.igem.org/mediawiki/2015/b/b7/KUL_SYM_VVN.png"
 +
                    style="margin-right:20px" width="150px">
 +
                <u>
 +
                    <b>Vera van Noort</b>, Center for Microbial and Plant Genetics
 +
                </u><br>
 +
                The research group led by Vera van Noort is interested in understanding
 +
                biological systems as a whole. They try to achieve this through computational
 +
                analysis of large-scale data generated by the ever growing number of new
 +
                technologies that can systematically measure the behaviour of multiple cellular
 +
                components, such as biochemical activities, biophysical properties, subcellular
 +
                localization and interaction. They use and develop new methods to integrate,
 +
                visualize and query the large amounts of information available and in such a way
 +
                come to new biological discoveries. A particular focus of the group is
 +
                proteomics and post-translational modifications.
 +
            </p>
 +
        </div>
 +
    </div>
 +
    <div class="whitespace"></div>
 +
    <br>
 +
    <br>
 +
    <br>
 +
    <br>
 +
    <br>
 +
    <br>
 +
    <br>
 +
    <div class="summarytext1">
 +
        <div class="part">
 +
            <p>
 +
                <img align="right" height=200px
 +
                    src="https://static.igem.org/mediawiki/2015/e/e2/KUL_SYM_YP.png"
 +
                    style="margin-left:20px;border:3px solid black;z-index:100;" width="140px">
 +
                <u>
 +
                    <b>Yves Peeters</b>, Laboratory of Biochemistry, Molecular and Structural Biology</u>
 +
                <br>
 +
                After completing his master thesis at KU Leuven, Yves obtained an IWT fellowship
 +
                for his PhD work in the field of synthetic biology. His primary interest goes to
 +
                DNA polymerases and their modifications towards creation of artificial nucleic
 +
                acids.
 +
            </p>
 +
        </div>
 +
    </div>
 +
    <br>
 +
    <br>
 +
    <br>
 +
    <br>
 +
   
 
<div class="summarytext1">
 
<div class="summarytext1">
−
<div class="part">
+
        <div class="part">
 +
            <h3 style="font-size:2.7em">Details</h3>
 +
            <br>
 +
            <div align="left">
 +
                <p>
 +
                    <b>DATE:</b><br>
 +
                    07.09.2015 10:00 – 19:00
 +
                    <br>
 +
                    <b>VENUE:</b>
 +
                    <br>
 +
                    KU Leuven Campus Arenberg, Celestijnenlaan 200A (Computer Science) aula 00.225,
 +
                    Heverlee, Belgium
 +
                    <br>
 +
                    <br>
 +
                    <br>
 +
                    <br>
 +
                </p>
 +
            </div>
 +
            <h3 style="font-size:2.7em">PROGRAM</h3><br>
 +
            <style type="text/css">
 +
                .tg {
 +
                    border-spacing: 0;
 +
                    border-color: #bbb;
 +
                    width=100%;
 +
                }
 +
                .tg td {
 +
                    font-family: Arial, sans-serif;
 +
                    font-size: 14px;
 +
                    padding: 10px 5px;
 +
                    border-style: none;
 +
                    overflow: hidden;
 +
                    word-break: normal;
 +
                    border-color: #bbb;
 +
                    color: #594f4f;
 +
                    background-color: #e0ffeb;
 +
                }
 +
                .tg th {
 +
                    font-family: Arial, sans-serif;
 +
                    font-size: 14px;
 +
                    font-weight: normal;
 +
                    padding: 10px 5px;
 +
                    border-style: none;
 +
                    overflow: hidden;
 +
                    word-break: normal;
 +
                    border-color: #bbb;
 +
                    color: #493f3f;
 +
                    background-color: #52bcec;
 +
                    line-height: 120%;
 +
                }
 +
                .tg .tg-title {
 +
                    font-weight: bold;
 +
                    font-size: 24px;
 +
                    text-align: center;
 +
                }
 +
                .tg .tg-grey {
 +
                    background-color: #c6dee9;
 +
                    text-align: left;
 +
                }
 +
                .tg .tg-lblue {
 +
                    background-color: #aae2fc;
 +
                    text-align: left;
 +
                }
 +
                .tg .tg-dblue {
 +
                    background-color: #89cfef;
 +
                    text-align: left;
 +
                }
 +
                @media screen and (max-width: 100%) {.tg {
 +
                        width: 100%;
 +
                    }
 +
                    .tg col {
 +
                        width: 100%;
 +
                    }
 +
                    .tg-wrap {
 +
                        overflow-x: auto;
 +
                        -webkit-overflow-scrolling: touch;
 +
                    }
 +
                }
 +
            </style>
 +
            <div class="tg-wrap" style="width:100%">
 +
                <table class="tg" style="width:100%">
 +
                    <tr>
 +
                        <th class="tg-title" colspan="3">KU Leuven iGEM 2015 Symposium
 +
                            <br>
 +
                            on Synthetic Biology, Cell Systems and Ethics in Biochemistry
 +
                            <br>
 +
                            Leuven 07.09.2015</th>
 +
                    </tr>
 +
                    <tr>
 +
                        <td class="tg-grey"></td>
 +
                        <td class="tg-grey">9:00-10:00</td>
 +
                        <td class="tg-grey">
 +
                            <b>Registration and welcome tea/coffee</b>
 +
                        </td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td class="tg-dblue" rowspan="6">Morning Block</td>
 +
                        <td class="tg-dblue">10:00-10:10</td>
 +
                        <td class="tg-dblue">Welcome words by Prof. Johan Robben</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td class="tg-dblue">10:10-10:35</td>
 +
                        <td class="tg-dblue">
 +
                            <b>Home speaker: Vera van Noort
 +
                            </b>
 +
                            <br>
 +
                            Center for Microbial and Plant Genetics<br>
 +
                            <i>"Lessons from systems biology of a minimal organism for synthetic biology"</i>
 +
                        </td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td class="tg-dblue">10:35-11:35</td>
 +
                        <td class="tg-dblue">
 +
                            <b>Keynote speaker: Victor Dillard</b>
 +
                            <br>
 +
                            Chief Operating Officer & Founder, Desktop Genetics<br>
 +
                            <i>
 +
                                "Through synthetic biology to entrepreneurship"</i>
 +
                        </td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td class="tg-dblue"></td>
 +
                        <td class="tg-dblue">
 +
                            <b>
 +
                                Presentation by the iGEM Teams</b>
 +
                        </td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td class="tg-dblue">11:40-11:50</td>
 +
                        <td class="tg-dblue">
 +
                            iGEM Paris-Saclay: "SafetE.coli"</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td class="tg-dblue">11:55-12:05</td>
 +
                        <td class="tg-dblue">
 +
                            iGEM TU Eindhoven: "Click Coli"</td>
 +
                    </tr>
  
−
<div class="onoffswitch">
+
                    <tr>
−
    <input type="checkbox" name="onoffswitch" class="onoffswitch-checkbox" id="EasySwitch" onchange="EasySwitch()" onmouseover="WhatSwitch()" style="cursor:Pointer;" title="Find out more about James Bond and his line of work">
+
                        <td class="tg-grey"></td>
−
    <label class="onoffswitch-label" for="EasySwitch">
+
                        <td class="tg-grey">12:05-13:05</td>
−
         <span class="onoffswitch-inner" title="Press me please"></span>
+
                        <td class="tg-grey">
−
         <span class="onoffswitch-switch" title="Press me please"></span>
+
                            <b>Lunch Break, networking</b>
−
     </label>
+
                        </td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td class="tg-lblue" rowspan="6">Early Afternoon Block</td>
 +
                        <td class="tg-lblue">13:05-14:05</td>
 +
                        <td class="tg-lblue">
 +
                            <b>Keynote speaker: Sebastian Maerkl
 +
                            </b>
 +
                            <br>
 +
                            École Polytechnique Fédérale de Lausanne, LBNC<br>
 +
                            <i>
 +
                                "Cell-Free synthetic biology"
 +
                            </i>
 +
                        </td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td class="tg-lblue">14:05-14:30</td>
 +
                        <td class="tg-lblue">
 +
                            <b>Home speaker: Yves Peeters</b><br>
 +
                            Laboratory of Biochemistry: Molecular and Structural Biology
 +
                            <br>
 +
                            <i>
 +
                                "Directed evolution of polymerases using synthetic biology methods"</i>
 +
                        </td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td class="tg-lblue"></td>
 +
                        <td class="tg-lblue">
 +
                            <b>
 +
                                Presentation by the iGEM Teams</b>
 +
                        </td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td class="tg-lblue">14:35-14:45</td>
 +
                        <td class="tg-lblue">
 +
                            iGEM Amsterdam: "[Photo]Synthetic Romance"</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td class="tg-lblue">14:50-15:00</td>
 +
                        <td class="tg-lblue">
 +
                            <del>
 +
                                iGEM TU Darmstadt: "Building with light/Labsurfing"
 +
                            </del>
 +
                        </td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td class="tg-lblue">15:05-15:20</td>
 +
                        <td class="tg-lblue">
 +
                            iGEM KU Leuven: "Spot E.Shape"</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td class="tg-grey"></td>
 +
                        <td class="tg-grey">15:25-15:50</td>
 +
                        <td class="tg-grey">
 +
                            <b>Tea/Coffee break</b>
 +
                        </td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td class="tg-dblue" rowspan="3">Evening block</td>
 +
                        <td class="tg-dblue">15:50-16:00</td>
 +
                        <td class="tg-dblue">Introduction of the debate experts: Prof. Bart De Moor (KU
 +
                            Leuven), Prof. Johan Robben (KU Leuven), Dr. Stijn Bruers (UGent), Prof. Vera
 +
                            van Noort (KU Leuven), Victor Dillard (Desktop Genetics).
 +
                            <br>
 +
                            Moderator: Prof. Piet Van der Meer (Ugent/VUB)
 +
                        </td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td class="tg-dblue">16:00-17:00</td>
 +
                        <td class="tg-dblue">A debate on the ethics in synthetic biology and biochemistry</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td class="tg-dblue">17:00-17:10</td>
 +
                        <td class="tg-dblue">Closing words</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td class="tg-grey"></td>
 +
                        <td class="tg-grey">17:10-19:00</td>
 +
                        <td class="tg-grey">
 +
                            <b>Wok and Talk Chinese dinner reception
 +
                            </b>
 +
                        </td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td class="tg-grey"></td>
 +
                        <td class="tg-grey">19:30</td>
 +
                        <td class="tg-grey">Leuven Kermis – Visit to Leuven Centrum for interested people</td>
 +
                    </tr>
 +
                </table>
 +
            </div>
 +
            <br>
 +
            <br>
 +
        </div>
 +
    </div>
 +
 
 +
    <div class="summarytext1">
 +
         <div class="part"> 
 +
        <br>
 +
        <br>
 +
        <h2 style="font-size:2.7em">Drinks and food</h2>
 +
         <p>
 +
            Beverages, lunch sandwiches and dinner-reception where be provided for all the
 +
            participants free of charge.
 +
        </p>
 +
        <br>
 +
        <br>
 +
        <p>
 +
            <h2 style="font-size:2.7em">Map</h2><br>
 +
            <img height=80%
 +
                src="https://static.igem.org/mediawiki/2015/1/17/KUL_Campus_Arenberg.png" width=100%>
 +
        </p>
 +
    </div>
 +
 
 +
    <br/>
 +
     <br/>
 
</div>
 
</div>
 +
   
 +
          <div class="subsections">
 +
            <div class="subsectionwrapper">
 +
                <div class="subimgrow">
 +
                    <div class="whitespace1"></div>
 +
                    <div class="subimg">
 +
                        <a href="https://2015.igem.org/Team:KU_Leuven/Outreach/Survey">
 +
                            <img
 +
                                src="https://static.igem.org/mediawiki/2015/3/36/KU_Leuven_Wiki_Button_-_Survey2.png"
 +
                                width="100%">
 +
                        </a>
 +
                    </div>
  
−
<br>
+
                    <div class="whitespace"></div>
−
<br>
+
  
 +
                    <div class="subimg">
 +
                        <a href="https://2015.igem.org/Team:KU_Leuven/Practices/Public">
 +
                            <img
 +
                                src="https://static.igem.org/mediawiki/2015/8/84/KU_Leuven_Wiki_Button_-_Outreach2.png"
 +
                                width="100%">
 +
                        </a>
 +
                    </div>
  
−
<!------------------------------------------------------Scientific----------------------------------------------------------->
+
                    <div class="whitespace"></div>
−
<div class="scientific">
+
−
<h2> Introduction </h2>
+
−
<p>
+
−
Intrigued by the patterns occuring in nature, we started our research to design possible interaction schemes and genetic circuits. Looking at specialised literature, we were able to find some interesting papers concerning the formation of patterns using
+
−
<i>Escherichia coli</i>
+
−
(<i>E. coli</i>). For example, Basu <i>et al.</i> created ring-like patterns based on chemical gradients of an acyl-homoserine lactone (OHHL) signal that is synthesized by ‘sender’ cells. In ‘receiver’ cells, designed genetic networks respond to differences in OHHL concentrations
+
−
<sup><a href="#Basu2005">[1]</a></sup>.
+
−
Liu <i> et al.</i> created periodic stripes of high and low cell densities of
+
−
<i>E. coli</i>, by inhibiting cell motility when cell density was high
+
−
<sup><a href="#Liu2011">[2]</a></sup>.
+
−
Combining our innovative and altered chemotaxis intercellular relationship with basic principles from both papers, allowed us to design our own circuit which will be elucidated in the following paragraphs.
+
−
</p>
+
−
</br>
+
−
</br>
+
−
+
  
−
<div class="center">
+
                    <div class="subimg">
−
<div id="image1">
+
                        <a href="https://2015.igem.org/Team:KU_Leuven/Practices/Ethics">
−
    <img src="https://static.igem.org/mediawiki/2015/1/17/KU_Leuven_Scheme_motility_bacteria.png" style="width:100%">
+
                            <img
−
<h3>  
+
                                src="https://static.igem.org/mediawiki/2015/7/70/KU_Leuven_Wiki_Button_-_Ethics2.png"
−
<b>Figure 1</b>
+
                                width="100%">
−
Signalling cascade for motility in <i> E.coli </i> </h3>
+
                        </a>
−
</div>
+
                    </div>
−
</div>
+
                    <div class="whitespace1"></div>
 +
                </div>
  
 +
                <div class="subtextrow">
 +
                    <div class="whitespace1"></div>
 +
                    <div class="subtext">
 +
                        <a href="https://2015.igem.org/Team:KU_Leuven/Outreach/Survey">
 +
                            <h2>Survey</h2>
 +
                            <p>We conducted a survey to shed light on the perception of synthetic biology in
 +
                                Belgium.
 +
                            </p>
 +
                        </a>
 +
                    </div>
  
−
</br>
+
                    <div class="whitespace"></div>
  
−
<h2> The designed circuit </h2>
+
                    <div class="subtext">
 +
                        <a href="  https://2015.igem.org/Team:KU_Leuven/Practices/Public">
 +
                            <h2>Public Engagement</h2>
 +
                            <p>How we engaged generally and intra university-wise.
 +
                            </p>
 +
                        </a>
 +
                    </div>
  
−
</br>
+
                    <div class="whitespace"></div>
−
   
+
−
<p>
+
−
Two different cell types, called A and B, interact and create patterns. In order to achieve the desired behavior, the cells used in our experiments are derived from
+
−
<i>Escherichia coli </i>
+
−
K12 and have specific knockouts. Cell type A has a deletion of
+
−
<i>tar</i> and
+
−
<i>tsr</i>
+
−
, whereas in cell type B both
+
−
<i>tar</i> and
+
−
<i>cheZ</i> are knocked out. Tar and Tsr are two major chemotactic receptors, guiding the cells towards nutrients. When a certain small molecule binds one of these receptors, a signalling cascade is initiated. In our specific case, cell’s A deletion of both
+
−
<i>tsr</i> and
+
−
<i>tar</i> causes them to be insensitive to leucine (as a repellent and attractant). Cell B on the other hand, who lost only Tar, is repelled by leucine.
+
−
<sup><a href="#Khan2004">[3]</a></sup>
+
−
Upon recognition of the small molecule (in this case leucine), the receptor transduces the signal through a set of Che proteins. This group of methylesterases and phosphatases regulates the rotation of the flagella (figure 1). In <i> E. coli </i>, the motors turn counterclockwise (CCW) in their default state, allowing the several filaments on a cell to join together in a bundle and propel the cell smoothly forward. The interaction of phospho-CheY and a motorprotein causes the motors to switch to clockwise (CW) rotation, inducing dissociation of the filament bundle and tumbling of the cell. (see figure 1)
+
−
<sup><a href="#Sarkar2010">[4]</a></sup>
+
−
To regulate CheY activity, a protein called CheZ removes a phosphate group and subsequently inhibits its activity. Cells lacking this protein  are not able to swim and will tumble excessively and incessantly
+
−
<sup><a href="#Kuo1987">[5]</a></sup>.
+
−
</br>
+
−
</br>
+
−
</p>
+
−
</br>
+
  
−
<div class="center">
+
                    <div class="subtext">
−
<div id="image2">
+
                        <a href="https://2015.igem.org/Team:KU_Leuven/Practices/Ethics">
−
            <a class="example-image-link" href="https://static.igem.org/mediawiki/2015/f/f7/KU_Leuven_PlasmidA_zoomed_noBG.png" data-lightbox="Plasmid A" data-title="Plasmid A"><img class="example-image" src="https://static.igem.org/mediawiki/2015/f/f7/KU_Leuven_PlasmidA_zoomed_noBG.png" alt="Plasmid A" width="49%" height="49%"></a>
+
                            <h2>Ethics</h2>
−
            <a class="example-image-link" href="https://static.igem.org/mediawiki/2015/e/ed/KU_Leuven_PlasmidB_zoomed_noBG.png" data-lightbox="Plasmid B" data-title="Plasmid B"><img class="example-image" src="https://static.igem.org/mediawiki/2015/e/ed/KU_Leuven_PlasmidB_zoomed_noBG.png" alt="Plasmid B" width="45%" height="45%"></a>
+
                            <p>
−
<h3> Figure 2. Left: plasmid A, Right: plasmid B. Click to enlarge </h3>
+
                                What we learned from a panel of experts we invited to discuss ethical and legal
−
          </div>
+
                                questions.
−
</div>
+
                            </p>
 +
                        </a>
 +
                    </div>
 +
                    <div class="whitespace1"></div>
 +
                </div>
  
−
</br>
+
                <div class="subimgreadmore">
−
<p>
+
                    <div class="whitespace1"></div>
−
Both cells are transfected with only one plasmid each (Figure 2). Both plasmids contain a temperature sensitive cI repressor protein, which is constitutively expressed. This repressor is only able to bind to the lambda promoter at temperatures below a certain threshold (between 30°C and 42°C). At elevated temperatures, cI is unable to bind to the promoter region enabeling RNA polymerase to start transcription.
+
                    <div class="subimgrm">
−
<sup><a href="#Villaverde1993">[6]</a></sup>  
+
                        <a href="https://2015.igem.org/Team:KU_Leuven/Outreach/Survey">
−
From the lambda promoter in cell A, two essential protagonists for our system are expressed: the OHHL-producing enzyme LuxI and the transcription activator LuxR
+
                            <div id="more">
−
<sup><a href="#Fuqua2001">[7]</a></sup>.
+
                                <img alt="Read more" height="40%"
−
On the contrary, cell B only contains the lambda promoter coupled to the <i>luxR</i> gene. In cell A, we coupled the expression of an autoaggregation factor Ag43-YFP and a leucine-producing enzyme Transaminase B to the OHHL-LuxR regulated promoter. Ag43 causes cells A to stick together and form clumps
+
                                    src="https://static.igem.org/mediawiki/2015/7/73/KUL_Wiki_Button_-_Read_more.png"
−
<sup><a href="#Ullet2006">[8]</a></sup>.
+
                                    width="85%">
−
At the same time, Transaminase B catalyses the production of the repellent leucine
+
                            </div>
−
<sup><a href="#Cox1989">[9]</a></sup>.  
+
                        </a>
−
The repellent has no impact on cell A, since it does not contain the necessary receptors  anymore. Cell B on the other hand is repelled by leucine, and contains the Lux promoter coupled to CheZ-GFP and the transcription repressor PenI. This repressor binds to the Pen-promoter
+
                    </div>
−
<sup><a href="#Wittman">[10]</a></sup>
+
−
, which regulates the transcription of a red fluorescent protein (RFP). The use of fluorescent fusion proteins specific for a certain state (aggregated, swimming, tumbling) facilitates the read-out of our patterns. In order to measure the concentration of the key proteins LuxI and LuxR, they were fused with a His-tag and an E-tag respectively. To ensure rapid degradation of proteins whose expression is dependent on OHHL concentration, an LVA tag was fused to CheZ, GFP and RFP. (figure 3)
+
−
</br>
+
−
</br>
+
−
</p>
+
−
<div class="center">
+
−
<div id="image3">
+
−
+
−
    <img src="https://static.igem.org/mediawiki/2015/4/4d/KU_Leuven_scheme_pattern_formation.png" style="width:100%">
+
−
<h3>
+
−
<div id=figure2> <b>Figure 3</b> </div>
+
−
The designed circuit </h3>
+
−
</div>
+
−
</div>
+
  
−
</br>
+
                    <div class="whitespace"></div>
−
<h2>Hypotheses</h2>
+
−
<p>
+
−
</br>
+
−
We expect that by raising the temperature, cells of type A will start to secrete OHHL. This OHHL will activate cells A to produce Ag43 and leucine and cells B to express CheZ-GFP and PenI. Cells A will start to form distinct spots on the agar plate and will emit yellow light (λ = 528 nm). At the same time, cells B will be repelled from cells A by leucine, the expression of PenI will repress the expression of RFP and thus the red color, whereas the expression of CheZ will enable the bacteria to swim and render them green. When they have swum too far away from cells A, the concentration of OHHL decreases to levels which are too low for LuxR to bind to the promotor. When LuxR cannot bind anymore, CheZ and PenI will no longer be expressed causing the bacteria to tumble incessantly and become red again.
+
−
</p>
+
  
−
</div>
+
                    <div class="subimgrm">
 +
                        <a href="https://2015.igem.org/Team:KU_Leuven/Practices/Public">
 +
                            <div id="more">
 +
                                <img alt="Read more" height="40%"
 +
                                    src="https://static.igem.org/mediawiki/2015/7/73/KUL_Wiki_Button_-_Read_more.png"
 +
                                    width="85%">
 +
                            </div>
 +
                        </a>
 +
                    </div>
  
−
<!------------------------------------------------------Easy-------------------------------------------------------------->
+
                    <div class="whitespace"></div>
−
<div class="easy">
+
  
−
<h2> Introduction </h2>
+
                    <div class="subimgrm">
−
<p>
+
                        <a href="https://2015.igem.org/Team:KU_Leuven/Practices/Ethics">
−
Scientists have been interested in pattern formation for a long time. Using different techniques, they were already able to generate nice and sometimes complex patterns on a petri dish (see figure 1). </p>
+
                            <div id="more">
−
<br>
+
                                <img alt="Read more" height="40%"
−
<div class="center">
+
                                    src="https://static.igem.org/mediawiki/2015/7/73/KUL_Wiki_Button_-_Read_more.png"
−
<div id="image1">
+
                                    width="85%">
−
    <img src="https://static.igem.org/mediawiki/2015/4/49/KU_Leuven_patternExample.png" style="width:100%">
+
                            </div>
−
<h3>  
+
                        </a>
−
<b>Figure 1</b>
+
                    </div>
−
</br>
+
                    <div class="whitespace1"></div>
−
Example of pattern formation on a petri dish (picture from Chenli Liu <i> et al. </i> ) </h3>
+
                </div>
−
</div>
+
            </div>
−
</div>
+
            <div class="whiterow"></div>
 +
            <div class="subsectionwrapper">
 +
                <div class="subimgrow">
 +
                    <div class="whitespace1"></div>
 +
                    <div class="subimg">
 +
                        <a href="https://2015.igem.org/Team:KU_Leuven/Practices/Education">
 +
                            <img
 +
                                src="https://static.igem.org/mediawiki/2015/7/78/KU_Leuven_Wiki_Button_-_Education2.png"
 +
                                width="100%">
 +
                        </a>
 +
                    </div>
  
−
<p>
+
                    <div class="whitespace"></div>
−
The basic principles behind this formation rely on two fundamental properties of bacteria: the communication between different cells and their ability to swim. Our project combines both properties, and adds another dimension to it. In our system, we also included a mechanism that causes the first type of bacteria to clump together and at the same time chase away the second type. According to our first rough models, this should allow to generate nice patterns.</p>
+
−
<br>
+
−
<h2> Our circuit </h2>
+
−
<p>
+
−
Our circuit consists of two different cells, custom-made for this project. We made the first one, called cell A, in this way that it is never repelled by itself or the other cell. The second one, called cell B, is unable to swim under normal circumstances and is sensitive to certain repelling molecules.  Each of these cells produce different proteins. These cellular workforces execute a variety of functions: </p>
+
−
<br>
+
−
<p>
+
  
−
Cell A contains two proteins (LuxI and LuxR).  LuxI produces a small molecule called OHHL. Binding of OHHL together with LuxR to a specific DNA is needed to start up the synthesis of a second group of proteins (Transaminase B and Ag43). Ag43 is some sort of molecular velcro, causing cell A to aggregate. Transaminase B makes the molecules that scare away cell B. </p>
+
                    <div class="subimg">
−
<br>
+
                        <a href="https://2015.igem.org/Team:KU_Leuven/Collaborations">
−
<p>
+
                            <img
 +
                                src="https://static.igem.org/mediawiki/2015/e/e0/KU_Leuven_Wiki_Button_-_Collaboration2.png"
 +
                                width="100%">
 +
                        </a>
 +
                    </div>
  
−
Cell B on the other hand only contains LuxR instead of both LuxI and LuxR. Because of this lack of the OHHL producing enzyme LuxI, the proteins whose production is LuxR and OHHL dependent (in this case CheZ and PenI) are only expressed when cell B is close to cell A. In this case, expression of CheZ restores the ability to swim, whereas PenI stops the synthesis of a red fluorescent protein. This red color indicates the standard and non-induced situation for cell B, and is used as an easy read-out to distinguish the different cells. </p>
+
                    <div class="whitespace"></div>
−
<br>
+
  
−
<div class="center">
+
                  <div class="subimg">
−
<div id="image3">
+
                        <a href="https://2015.igem.org/Team:KU_Leuven/Practices">
−
    <img src="https://static.igem.org/mediawiki/2015/4/4d/KU_Leuven_scheme_pattern_formation.png" style="width:100%">
+
                            <img src="https://static.igem.org/mediawiki/2015/c/cb/KUL_Wiki_Button_-_Back.png"
−
<h3>
+
                                width="100%">
−
<div id=figure2> <b>Figure 2</b> </div>
+
                        </a>
−
The designed circuit </h3>
+
                    </div>
−
</div>
+
−
</div>
+
  
−
<p>
+
                    <div class="whitespace1"></div>
−
In order to visualize induced patterns nicely, we also gave some proteins (Ag43 and CheZ) a color by fusing two fluorescent proteins to them. The whole system is temperature sensitive, since the expression of LuxI and LuxR starts when temperatures are elevated to around 42°C. To achieve this we include a protein (cI) that binds to the DNA at a specific site and blocks production of LuxI and/or LuxR. However, at higher temperatures, this protein starts to loose its three dimensional structure and the blockade is lifted initiating the whole pattern formation process. Figure 2 gives a summary of our circuit</p>
+
                </div>
−
<br>
+
−
<h2> Expectations </h2>
+
−
<p>
+
−
So, in theory, when we raise the temperature, the pattern formation will start. Cell A will start to aggregate and will obtain a yellow color. At the same time, it will produce the repellents for cell B. In cell B, the red color will disappear, and the swimming motility will be restored. A green color will appear simultaneously. However, when cell B is too far from cell A, the second group of proteins is no longer expressed, leading to a subsequent loss of motility and green color, and the return of the red fluorescent color. </p>
+
−
<br>
+
−
<p>
+
−
Normally, this will produce distinct yellow spots, with a green circle around of swimming cells B. At the edges, the immotile red cells B will start to appear. </p>
+
−
<br>
+
−
<br>
+
−
</div>
+
−
</div>
+
−
</div>
+
−
<!------------------------------------------------------References--------------------------------------------------------->
+
−
<div class="scientific">  
+
−
<div class="summaryheader">
+
−
    <div class="summaryimg">
+
−
  <img src="https://static.igem.org/mediawiki/2015/b/b7/KU_Leuven_Researchbanner.jpg" width="100%">
+
−
  <div class="head">
+
−
      <h2> References </h2>
+
−
    </div>
+
−
  </div>
+
−
  </div>
+
−
<div class="summarytext2">
+
−
<div class="part">
+
  
−
<p id="ref1"> <a name="Basu2005"></a><a href="http://dx.doi.org/10.1038/nature03461#" target="_blank">[1] Subhayu Basu, Yoram Gerchman, Cynthia H. Collins, Frances H. Arnold & Ron Weiss “A synthetic multicellular system for programmed pattern formation” Nature 434 (2005): 1130-1134. </a></p>
+
                <div class="subtextrow">
−
</br>
+
                    <div class="whitespace1"></div>
−
<p id="ref2"><a name="Liu2011"></a><a href="http://dx.doi.org/10.1126/science.1209042" target="_blank">[2] Chenli Liu, Xiongfei Fu, Lizhong Liu, Xiaojing Ren, Carlos K.L. Chau, Sihong Li, Lu Xiang, Hualing Zeng, Guanhua Chen, Lei-Han Tang, Peter Lenz, Xiaodong Cui, Wei Huang, Terence Hwa, Jian-Dong Huang” Sequential Establishment of Stripe Patterns in an Expanding Cell Population” Science 334 no. 6053 (2011) : 238-241. </a></p>
+
                    <div class="subtext">
−
</br>
+
                        <a href="https://2015.igem.org/Team:KU_Leuven/Practices/Education">
−
<p id="ref3"><a name="Khan2004"></a><a href="http://dx.doi.org/10.1128/JB.186.2.588-592.2004" target="_blank">[3] Khan, S., and D. R. Trentham. “Biphasic Excitation by Leucine in Escherichia Coli Chemotaxis.” Journal of Bacteriology 186, no. 2 (2004): 588-592. </a></p>
+
                            <h2>Education</h2>
−
</br>
+
                            <p>
−
<p id="ref4"><a name="Sarkar2010"></a> <a href="http://dx.doi.org/10.1073/pnas.1000935107" target="_blank">[4] Sarkar MK, Paul K, Blair D , “Chemotaxis signaling protein CheY binds to the rotor protein FliN to control the direction of flagellar rotation in Escherichia coli.” PNAS 107, no. 20 (2010): 9370-9375. </a></p>
+
                                We went teaching and devised an educational card game, which is fun to play.
−
</br>
+
                            </p>
−
<p id="ref5"><a name="Kuo1987"></a><a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC211935/" target="_blank">[5] Kuo, Scot C., and D. E. Koshland. “Roles of cheY and cheZ Gene Products in Controlling Flagellar Rotation in Bacterial Chemotaxis of Escherichia Coli.” Journal of Bacteriology 169, no. 3 (1987): 1307–14. </a></p>
+
                        </a>
−
</br>
+
                    </div>
−
<p id="ref6"><a name="Villaverde1993"></a><a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC182479/" target="_blank">[6] Villaverde, A., A. Benito, E. Viaplana, and R. Cubarsi. “Fine Regulation of cI857-Controlled Gene Expression in Continuous Culture of Recombinant Escherichia Coli by Temperature.” Applied and Environmental Microbiology 59, no. 10 (1993): 3485–87.</a></p>
+
−
</br>
+
−
<p id="ref7"><a name="Fuqua2001"></a><a href="http://dx.doi.org/10.1146/annurev.genet.35.102401.090913" target="_blank">[7] Clay Fuqua , Matthew R. Parsek , and E. Peter Greenberg “Regulation of Gene Expression by Cell-to-Cell Communication: Acyl-Homoserine Lactone Quorum Sensing” , Annu. Rev. Genet. (2001) 35:439–68 </a></p> 
+
−
</br>
+
−
<p id="ref8"><a name="Ulett2006"></a><a href="http://dx.doi.org/10.1099/mic.0.28607-0" target="_blank">[8] Ulett, G. C. “Antigen-43-Mediated Autoaggregation Impairs Motility in Escherichia Coli.” Microbiology 152, no. 7 (2006): 2101–2110. </a></p>
+
−
</br>
+
−
<p id="ref9"> <a name="Cox1989"></a><a href="http://www.ncbi.nlm.nih.gov/pubmed/3315862" target="_blank"> [9] James L. Cox, Betty J. Cox , Vincenzo Fidanza , David H. Calhoun “The complete nucleotide sequence of the iZvGMEDA cluster of Escherichia coli K-12” Gene 56, Issues 2–3 (1987): 185–198. </a></p>
+
−
</br>
+
−
<p id="ref10"><a name="Wittman1993"></a><a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC206883/" target="_blank">[10] Wittman, V., H. C. Lin, and H. C. Wong. “Functional Domains of the Penicillinase  Repressor of Bacillus Licheniformis.” Journal of Bacteriology 175, no. 22 (1993):  7383–7390. </a></p>
+
−
</div>
+
−
</div>
+
−
</div>
+
−
<!--------------------------------------------------Foot don't touch!------------------------------------------------------>
+
  
 +
                    <div class="whitespace"></div>
  
−
<div class="subsections">
+
                    <div class="subtext">
−
<div class="subsectionwrapper">
+
                        <a href="https://2015.igem.org/Team:KU_Leuven/Collaborations">
−
<div class="subimgrow">
+
                            <h2>Collaborations</h2>
−
<div class="subimg">
+
                            <p>
−
<img src="https://static.igem.org/mediawiki/2015/4/4e/KU_Leuven_Gblocks.jpg" width="100%">
+
                                We worked together with other teams to improve both projects.
−
</div>
+
                            </p>
 +
                        </a>
 +
                    </div>
  
−
<div class="whitespace">
+
                    <div class="whitespace"></div>
−
</div>
+
  
−
<div class="subimg">
+
                    <div class="subtext">
−
<img src="https://static.igem.org/mediawiki/2015/3/32/KU_Leuven_AgarPlates.jpg" width="100%">
+
−
</div>
+
  
−
<div class="whitespace">
+
                        <a href="https://2015.igem.org/Team:KU_Leuven/Practices">
−
</div>
+
                            <h2>Back</h2>
 +
                            <p>
 +
                                Go back to the Outreach page.
 +
                            </p>
 +
                        </a>
 +
                    </div>
  
−
<div class="subimg">
+
                    <div class="whitespace1"></div>
−
<img src="https://static.igem.org/mediawiki/2015/b/b7/KU_Leuven_Motility2.jpg" width="92.4%">
+
                </div>
−
</div>
+
  
−
<div class="whitespace">
+
                <div class="subimgreadmore">
−
</div>
+
                    <div class="whitespace1"></div>
 +
                    <div class="subimgrm">
 +
                        <a href="https://2015.igem.org/Team:KU_Leuven/Practices/Education">
 +
                            <div id="more">
 +
                                <img alt="Read more" height="40%"
 +
                                    src="https://static.igem.org/mediawiki/2015/7/73/KUL_Wiki_Button_-_Read_more.png"
 +
                                    width="85%">
 +
                            </div>
 +
                        </a>
 +
                    </div>
  
−
<div class="subimg">
+
                    <div class="whitespace"></div>
−
<a href="https://2015.igem.org/Team:KU_Leuven/Wetlab">
+
−
<!---<img src="https://static.igem.org/mediawiki/2015/2/2b/KU_Leuven_Monocotyl.jpg" width="100%" height="100%" >-->
+
−
</a>
+
−
</div>
+
−
</div>
+
  
−
<div class="subtext">
+
                    <div class="subimgrm">
−
<h2>Parts</h2>
+
                        <a href="https://2015.igem.org/Team:KU_Leuven/Collaborations">
−
<p>
+
                            <div id="more">
−
Coming Soon
+
                                <img alt="Read more" height="40%"
−
</p>
+
                                    src="https://static.igem.org/mediawiki/2015/7/73/KUL_Wiki_Button_-_Read_more.png"
−
</div>
+
                                    width="85%">
 +
                            </div>
 +
                        </a>
 +
                    </div>
  
−
<div class="whitespace">
+
                    <div class="whitespace"></div>
−
</div>
+
  
−
<div class="subtext">
+
                    <div class="subimgrm">
−
<h2>Methods</h2>
+
                        <a href="https://2015.igem.org/Team:KU_Leuven/Practices">
−
<p>  
+
                            <div id="back">
−
Coming Soon
+
                                <img alt="Read more" height="40%"
−
</p>
+
                                    src="https://static.igem.org/mediawiki/2015/7/73/KUL_Wiki_Button_-_Read_more.png"
−
</div>
+
                                    width="85%">
 +
                            </div>
 +
                        </a>
 +
                    </div>
  
−
<div class="whitespace">
 
−
</div>
 
  
−
<div class="subtext">
+
                    <div class="whitespace1"></div>
−
<h2>Results</h2>
+
                </div>
−
<p>
+
−
Coming Soon
+
−
</p>
+
−
</div>
+
  
−
<div class="whitespace">
+
                <!--          Mobile starts here!!!        -->
−
</div>
+
                <div class="subimgrowm">
 +
                    <a href="https://2015.igem.org/Team:KU_Leuven/Outreach/Survey">
 +
                        <div class="subimgm">
 +
                            <b>Survey</b>
 +
                            <img
 +
                                src="https://static.igem.org/mediawiki/2015/3/36/KU_Leuven_Wiki_Button_-_Survey2.png"
 +
                                width="100%">
 +
                        </div>
  
−
<div class="subtext">
+
                        <div class="whitespace"></div>
−
<a href="https://2015.igem.org/Team:KU_Leuven/Research">
+
 
−
  <h2>Back</h2>
+
                        <div class="subtextm">
 +
                            <p>
 +
                                We did a survey to shed light on the perception of synthetic biology in belgium.
 +
                            </p>
 +
                        </div>
 +
                    </a>
 +
                </div>
 +
 
 +
                <div class="subimgrowm">
 +
                    <div class="whiterow"></div>
 +
                </div>
 +
 
 +
                <div class="subimgrowm">
 +
                    <a href="https://2015.igem.org/Team:KU_Leuven/Practices/Public">
 +
                        <div class="subimgm">
 +
                            <b>Public Engagement</b>
 +
                            <img
 +
                                src="https://static.igem.org/mediawiki/2015/8/84/KU_Leuven_Wiki_Button_-_Outreach2.png"
 +
                                width="100%">
 +
                        </div>
 +
 
 +
                        <div class="whitespace"></div>
 +
 
 +
                        <div class="subtextm">
 +
                            <p>
 +
                                How we engaged generally and intra university-wise.
 +
                            </p>
 +
                        </div>
 +
                    </a>
 +
                </div>
 +
 
 +
                <div class="subimgrowm">
 +
                    <div class="whiterow"></div>
 +
                </div>
 +
 
 +
                <div class="subimgrowm">
 +
                    <a href="https://2015.igem.org/Team:KU_Leuven/Practices/Ethics">
 +
                        <div class="subimgm">
 +
                            <b>Ethics</b>
 +
                            <img
 +
                                src="https://static.igem.org/mediawiki/2015/7/70/KU_Leuven_Wiki_Button_-_Ethics2.png"
 +
                                width="100%">
 +
                        </div>
 +
 
 +
                        <div class="whitespace"></div>
 +
 
 +
                        <div class="subtextm">
 +
                            <p>
 +
                                We invited a panel of experts to discuss ethical questions with us.
 +
                            </p>
 +
                        </div>
 +
                    </a>
 +
                </div>
 +
 
 +
                <div class="subimgrowm">
 +
                    <div class="whiterow"></div>
 +
                </div>
 +
 
 +
                <div class="subimgrowm">
 +
                    <a href="https://2015.igem.org/Team:KU_Leuven/Practices/Education">
 +
                        <div class="subimgm">
 +
                            <b>Education</b>
 +
                            <img
 +
                                src="https://static.igem.org/mediawiki/2015/7/78/KU_Leuven_Wiki_Button_-_Education2.png"
 +
                                width="100%">
 +
                        </div>
 +
 
 +
                        <div class="whitespace"></div>
 +
 
 +
                        <div class="subtextm">
 +
                            <p>
 +
                                We went teaching and devised an educational card game, which is fun to play.
 +
                            </p>
 +
                        </div>
 +
                    </a>
 +
                </div>
 +
 
 +
                <div class="subimgrowm">
 +
                    <div class="whiterow"></div>
 +
                </div>
 +
 
 +
                <div class="subimgrowm">
 +
                    <a href="https://2015.igem.org/Team:KU_Leuven/Collaborations">
 +
                        <div class="subimgm">
 +
                            <b>Collaborations</b>
 +
                            <img
 +
                                src="https://static.igem.org/mediawiki/2015/e/e0/KU_Leuven_Wiki_Button_-_Collaboration2.png"
 +
                                width="100%">
 +
                        </div>
 +
                        <div class="whitespace"></div>
 +
 
 +
                        <div class="subtextm">
 +
                            <p>
 +
                                We worked together with other teams to improve both our projects.
 +
                            </p>
 +
                        </div>
 +
                    </a>
 +
                </div>
 +
 
 +
                <div class="subimgrowm">
 +
                    <div class="whiterow"></div>
 +
                </div>
 +
 
 +
               
 +
                <div class="subimgrowm">
 +
                    <a href="https://2015.igem.org/Team:KU_Leuven/Practices">
 +
                        <div class="subimgm">
 +
                            <b>Back</b>
 +
                            <img src="https://static.igem.org/mediawiki/2015/c/cb/KUL_Wiki_Button_-_Back.png"
 +
                                width="100%">
 +
                        </div>
 +
                        <div class="whitespace"></div>
 +
 
 +
                        <div class="subtextm">
 +
                            <p>
 +
                                Go back to the Outreach page.
 +
                            </p>
 +
                        </div>
 +
                    </a>
 +
                </div>
 +
 
 +
            </div>
 +
        </div>
 +
    </div>
 +
 
 +
 
 +
  <div id="summarywrapper">
 +
        <div class="summary">
 +
            <h3>
 +
                Contact
 +
            </h3>
 +
            <p style="font-size:1.3em; text-align: center">
 +
                Address: Celestijnenlaan 200G room 00.08 - 3001 Heverlee<br>
 +
                Telephone: +32(0)16 32 73 19<br>
 +
                Email: igem@chem.kuleuven.be<br>
 +
            </p>
 +
        </div>
 +
    </div>
 +
 
 +
<div class="whiterow"></div>
 +
 
 +
    <div id="footer">
 +
        <div class="rowfooter">
 +
            <div class="emptyfooter"></div>
 +
            <div id="bioscenter">
 +
                <a href="http://www.kuleuven.be/bioscenter/"><img alt="bioSCENTer"
 +
                    src="https://static.igem.org/mediawiki/2015/3/3c/KU_Leuven_Logo_BioSCENTer.png"
 +
                    width="95%"></a>
 +
            </div>
 +
            <div class="emptyfooter"></div>
 +
        </div>
 +
 
 +
        <div class="rowfooter">
 +
            <div id="lrd">
 +
                <a href="http://lrd.kuleuven.be/"><img alt="KU Leuven Research & Development"
 +
                    src="https://static.igem.org/mediawiki/2015/b/b4/KU_Leuven_HR_LRD_CMYK_LOGO.jpg"
 +
                    width="95%"></a>
 +
            </div>
 +
            <div id="youreca">
 +
                <a href="http://www.kuleuven.be/onderzoek/youreca/"><img alt="YOURECA"
 +
                    src="https://static.igem.org/mediawiki/2015/4/4f/KU_Leuven_YOURECA.png" width="95%"></a>
 +
            </div>
 +
            <div id="solvay">
 +
                <a href="http://www.solvay.com/"><img alt="SOLVAY"
 +
                    src="https://static.igem.org/mediawiki/2015/8/89/KU_Leuven_Solvay_Transparant.png"
 +
                    width="95%"></a>
 +
            </div>
 +
            <div id="medicine">
 +
                <a href="http://gbiomed.kuleuven.be/english"><img alt="Faculty of Medicine"
 +
                    src="https://static.igem.org/mediawiki/2015/8/83/KU_Leuven_Biomedische_Wetenschappen_logo_.png"
 +
                    width="95%"></a>
 +
            </div>
 +
        </div>
 +
 
 +
        <div class="rowfooter">
 +
            <div id="imec">
 +
                <a href="http://www2.imec.be/be_en/home.html"><img alt="imec"
 +
                    src="https://static.igem.org/mediawiki/2015/7/70/KU_Leuven_Imec_logo_transparant.png"
 +
                    width="95%"></a>
 +
            </div>
 +
            <div id="genzyme">
 +
                <a href="http://www.genzyme.be/"><img alt="Genzyme"
 +
                    src="https://static.igem.org/mediawiki/2015/b/b7/KU_Leuven_Genzyme_logo_transparant.png"
 +
                    width="95%"></a>
 +
            </div>
 +
            <div id="eppendorf">
 +
                <a href="https://www.eppendorf.com/BE-en/"><img alt="Eppendorf"
 +
                    src="https://static.igem.org/mediawiki/2015/9/96/KU_Leuven_Logo_Eppendorf_transparant.png"
 +
                    width="95%"></a>
 +
            </div>
 +
  <div id="saillart">
 +
      <a href="http://www.glasatelier-saillart.be/English/english.html"><img src="https://static.igem.org/mediawiki/2015/c/ce/KU_Leuven_Sponsor_Saillard.png" alt="Glasatelier Saillart" width="95%"></a>
 +
  </div>
 +
            <div id="kuleuven">
 +
                <a href="http://www.kuleuven.be/english"><img alt="bioSCENTer"
 +
                    src="https://static.igem.org/mediawiki/2015/0/08/KULEUVEN_groot.png" width="95%"></a>
 +
            </div>
 +
            <div class="spacefooter"></div>
 +
            <div id="gips">
 +
                <a href="http://www.gipsmineral.de/"><img alt="Gips Mineral"
 +
                    src="https://static.igem.org/mediawiki/2015/3/3d/KU_Leuven_Gips_Mineral_logo_transparant.png"
 +
                    width="95%"></a>
 +
            </div>
 +
            <div id="kolo">
 +
                <a><img alt="Ko-Lo Instruments"
 +
                    src="https://static.igem.org/mediawiki/2015/1/15/KUL_Ko-Lo_Instruments_logo_transparant.png"
 +
                    width="95%"></a>
 +
            </div>
 +
        </div>
 +
 
 +
        <div class="rowfooter">
 +
            <div id="bioke">
 +
                <a href="https://www.bioke.com/"><img alt="Bioké"
 +
                    src="https://static.igem.org/mediawiki/2015/e/e1/KUL_Biok%C3%A9_logo_transparant.png"
 +
                    width="95%"></a>
 +
            </div>
 +
          <div class="logonormal">
 +
<div id="regensys">
 +
      <a href="http://regenesys.eu/"><img src="https://static.igem.org/mediawiki/2015/e/eb/KU_Leuven_Logo_Regenesys_Transparant.png" alt="Regenesys" width="95%"></a>
 +
  </div>
 +
<div class="whiterow"></div>
 +
  <div id="thermofisher">
 +
      <a href="https://www.fishersci.com/us/en/home.html"><img src="https://static.igem.org/mediawiki/2015/a/aa/KUL_Fischer_Scientific_logo_transparant.png" alt="Thermo Fisher Scientific" width="95%"></a>
 +
  </div>
 +
</div>
 +
<div class="logonormal2">
 +
<div id="vwr">
 +
      <a href="https://be.vwr.com/store/?&_requestid=866148&_DARGS=/store/cms/be.vwr.com/nl_BE/header_20159241139103.jsp.1_AF&_dynSessConf=4047468000326453053&targetURL=/store/%3F%26_requestid%3D866148&lastLanguage=en&/vwr/userprofiling/EditPersonalInfoFormHandler.updateLocale=&_D%3AcurrentLanguage=+&currentLanguage=en&_D%3AlastLanguage=+&_D%3A/vwr/userprofiling/EditPersonalInfoFormHandler.updateLocale=+"><img src="https://static.igem.org/mediawiki/2015/8/8d/KU_Leuven_Logo_VWR_transparant_.png" alt="VWR" width="95%"></a>
 +
  </div>
 +
<div class = "whiterow"></div>
 +
<div id="lgc">
 +
<a href="http://www.lgcgroup.com/our-science/genomics-solutions/#.Vfx9V9yLTIU">
 +
                <img src="https://static.igem.org/mediawiki/2015/e/e6/KU_Leuven_LOGO_LGC.png" alt="LGC Genomics" width="80%">
 
</a>
 
</a>
 
</div>
 
</div>
 
</div>
 
</div>
 +
            <div id="footerimg">
 +
                <img
 +
                    src="https://static.igem.org/mediawiki/2015/b/b9/KU_Leuven_Zebra_spots_wiki_footer_main.png"
 +
                    width="95%">
 +
            </div>
 +
<div class="logonormal2">
 +
<div id="gimv">
 +
      <a href="http://www.gimv.com/en"><img src="https://static.igem.org/mediawiki/2015/a/ac/KU_Leuven_Logo_Gimv_Transparant.png" alt="Gimv" width="95%"></a>
 +
  </div>
 +
<div class = "whiterow"></div>
 +
<div id="sopach">
 +
      <a href="http://www.sopachem.com/"><img src="https://static.igem.org/mediawiki/2015/5/55/KU_Leuven_Sopachem.jpeg" alt="Sopachem" width="95%"></a>
 +
  </div>
 
</div>
 
</div>
 +
            <div id="machery">
 +
                <a href="http://www.filterservice.be/"><img alt="Machery Nagel"
 +
                    src="https://static.igem.org/mediawiki/2015/4/41/KU_Leuven_Macherey_Nagel_logo_transparant.png"
 +
                    width="95%"></a>
 +
            </div>
 +
            <div class="logosmall">
 +
    <div id="sigma">
 +
      <a href="https://www.sigmaaldrich.com/belgium-nederlands.html"><img src="https://static.igem.org/mediawiki/2015/4/4b/KUL_Sigma-Aldrich_logo_transparant.png" alt="Sigma-Aldrich" width="95%"></a>
 +
    </div>
 +
    <div class="whiterow"></div>
 +
    <div id="egilab">
 +
      <a href="http://www.egilabo.be/"><img src="https://static.igem.org/mediawiki/2015/e/e9/KUL_Egilabo_logo_transparant.png" alt="Egilabo" width="95%"></a>
 +
    </div>
 +
    <div class="whiterow"></div>
 +
    <div id="novolab">
 +
      <a href="https://www.novolab.be/"><img src="https://static.igem.org/mediawiki/2015/4/4c/KU_Leuven_Novalab.png" alt="Novolab" height="95%"></a>
 +
    </div>
 +
  </div>
 +
        </div>
  
−
<div class="center" id="footer">
+
     </body>
−
<div class="sponsorsleft">
+
−
  <div id="kuleuven">
+
−
  <a href="http://www.kuleuven.be/">
+
−
  <img src="https://static.igem.org/mediawiki/2015/0/08/KULEUVEN_groot.png" alt="KU Leuven" width="100%">
+
−
  </a>
+
−
  </div>
+
−
  <div id="krd">
+
−
  <a href="http://lrd.kuleuven.be/">
+
−
  <img src="https://static.igem.org/mediawiki/2015/b/b4/KU_Leuven_HR_LRD_CMYK_LOGO.jpg" alt="KU Leuven Research and Development" width="100%">
+
−
  </a>
+
−
  </div>
+
−
  <div id="youreca">
+
−
  <a href="http://www.kuleuven.be/onderzoek/youreca/">
+
−
  <img src="https://static.igem.org/mediawiki/2015/4/4f/KU_Leuven_YOURECA.png" alt="YOURECA" width="100%">
+
−
  </a>
+
−
  </div>
+
−
</div>
+
−
<div id="footercenter" >
+
−
  <div id="bioscenter">
+
−
  <a href="http://www.kuleuven.be/bioscenter/">
+
−
     <img src="https://static.igem.org/mediawiki/2015/3/3c/KU_Leuven_Logo_BioSCENTer.png" alt="bioSCENTer" width="100%">
+
−
  </a> 
+
−
  </div>
+
−
  <div id="footerimg">
+
−
  <img src="https://static.igem.org/mediawiki/2015/b/b9/KU_Leuven_Zebra_spots_wiki_footer_main.png" width="100%">
+
−
  </div>
+
−
</div>
+
−
<div class="sponsorsright">
+
−
  <div id="medicine">
+
−
  <a href="http://gbiomed.kuleuven.be/english">
+
−
  <img src="https://static.igem.org/mediawiki/2015/2/22/KU_Leuven_Medicine.png" alt="Faculty of Medicine" width="100%">
+
−
  </a>
+
−
  </div>
+
−
  <div id="solvay">
+
−
  <a href="http://www.solvay.com/">
+
−
  <img src="https://static.igem.org/mediawiki/2015/8/89/KU_Leuven_Solvay_Transparant.png" alt="SOLVAY" width="100%">
+
−
  </a>
+
−
  </div>
+
−
</div>
+
−
</div>
+
−
 
+
−
 
+
−
<!--------------------------------------------------EasySwitch-------------------------------------------------------------->
+
−
<script language="javascript" type="text/javascript">
+
−
function EasySwitch(){
+
−
var lckv= document.getElementById("EasySwitch").checked;
+
−
if (lckv==true)
+
−
{
+
−
$('.easy').css('display', 'inline');
+
−
$('.scientific').css('display', 'none');
+
−
}
+
−
else
+
−
{
+
−
$('.easy').css('display', 'none');
+
−
$('.easyleft').css('display', 'none');
+
−
$('.scientific').css('display', 'inline');
+
−
}
+
−
}
+
−
 
+
−
$( "#Switch" ).mouseenter(function() {
+
−
      $("#SwitchText").show();
+
−
});
+
−
$( "#Switch" ).mouseleave(function() {
+
−
    $("#SwitchText").hide();
+
−
});
+
−
</script>
+
−
</body>
+
 
</html>
 
</html>

Latest revision as of 10:04, 20 October 2015

Logo

Symposium

Event summary

On September the 7th, 2015 we organized the KU Leuven iGEM 2015 Symposium on Synthetic Biology, Cell Systems and Ethics in Biochemistry. Hosted guests included 4 neighbouring iGEM teams, academic staff, students, sponsors, and iGEM supporters from 6 different European countries. During this full-day event, the participants had a chance to attend the lectures by home speakers as well as by invited international keynote speakers from both the academia and the industry. The iGEM teams presented their research, and probably the most awaited part - the debate on ethics in biochemistry with panel of well selected experts lead by an experienced professional moderator - took place in the afternoon. To fulfil all the needs, goodie-bags, drinks, lunch, and dinner were provided to our guests. The day finished with a sightseeing walk around Leuven centre. Participation was free of charge. We hosted almost a hundred guests and received a very positive feedback.



Evaluation form quotes

"Very professionally organized. Very well run. Great speakers - diverse agenda."
"The lectures were super interesting!"
"Great symposium, I loved it!!"
"Amazing keynote speakers and a fantastic moderator. Everything was perfectly organized.”
"Food was very good!"
"It was the best symposium I have ever attended!!! Outstanding organization!!!"
"Very good symposium. The keynote speakers were marvellous, the debate was interesting, with panel members who were very well selected!"

Home speaker Vera van Noort talked about lessons from systems biology of a minimal organism for synthetic biology. In the EMBL, where she worked, they wanted to completely describe one organism, namely Mycoplasma pneumoniae. For complete understanding, three different levels were studied:
The metabolism, transcription (regulation) and protein complexes. The take-home messages for synthetic biology in short: transcription regulation is more than operons and transcription factors; enzymes can catalyse multiple reactions and are organized in multi-subunit complexes; proteins can be part of multiple complexes and can be regulated by post-translational modifications and small peptides are essential.

Victor Dillard, the first keynote speaker, is the founder of Desktop Genetics. The company is building software for biologists, with a focus on synthetic biology. The software helps to improve the efficiency and to lower the costs. He explained that genome editing remains hard and became a design and software challenge, and not purely biological challenge. This is because genome editing needs to be accurate, precise, effective and rapid.

Sebastian Maerkl, the second keynote speaker, discussed the topic cell-free synthetic biology. He explained that microfluidics with cell-free lysate can be used for rapid prototyping of biological systems. One of the advantages is that it has defined and controllable reaction conditions. In vitro prototyping is used to speed up research in synthetic biology. The pipeline of cell-free synthetic biology: design a biological circuit, build the circuit, test parts and circuits, characterize working circuits, clone and implement in vivo.

The second home speaker, Yves Peeters, gave a talk about directed evolution of polymerases using synthetic biology methods. The research of Yves Peeters is part of the research domain Xenobiology, creating alternative life, one of the approaches of synthetic biology. XNA, also called orthogonal DNA, is designed by several labs using different strategies. Making organisms with XNA will be an ultimate biosafety tool for synthetic biology. Before being able to have a liveable organism that uses XNA, there is a need for polymerases recognizing the specific XNA. To create the wanted polymerases, Yves uses directed evolution, including mutagenesis, screening, amplification and iteration of the most active enzymes.



Photo of the participating speakers and iGEM teams at the symposium.

Photo of the participating speakers and iGEM teams at the symposium. Click and on image and use arrow buttons to browse trough more pictures.

PARTICIPATING iGEM TEAMS


Speakers


Sebastian Maerkl, École Polytechnique Fédérale de Lausanne, The Laboratory of Biological Network Characterization (LBNC)
Sebastian Maerkl's lab conducts research at the interface of engineering and biology and is active in the areas of systems biology, synthetic biology and molecular diagnostics. They are driven by the desire to learn how to rationally design and engineer biological systems. Sebastian Maerkl’s research aims to develop new microfluidic technologies and apply them to solve biological problems. His rare expertise allows him to combine the design of new tools with advanced research in biology. Sebastian Maerkl is internationally recognized for his many outstanding contributions. Particularly in combining synthetic biology and computational systems with microfluidics, he demonstrated that the expression of genes in vivo can be provided based on the binding energy profiles in vitro. His studies will focus on five areas: the bioengineering of biosystems, the engineering of transcriptional regulatory networks, the engineering of genes and genomes, the engineering of biological systems de novo and the development of a new generation of diagnostic devices.





Victor Dillard, Chief Operating Officer & Founder, Desktop Genetics
Victor obtained his masters in chemical engineering with honours at Imperial College London before completing a specialist biotechnology and business masters with distinction at the University of Cambridge. Since graduating, Victor founded Desktop Genetics with a vision to change modern biotech R&D and enable rapid and accurate end-to-end genome engineering experiments through their proprietary software platform. Within two years of founding Desktop Genetics, Victor has raised over $600,000 of private equity and grant financing, and delivered over $400,000 of revenue. Today, Victor heads the company's business development and operations and is leading the product and technology expansion into CRISPR and genome editing.





Vera van Noort, Center for Microbial and Plant Genetics
The research group led by Vera van Noort is interested in understanding biological systems as a whole. They try to achieve this through computational analysis of large-scale data generated by the ever growing number of new technologies that can systematically measure the behaviour of multiple cellular components, such as biochemical activities, biophysical properties, subcellular localization and interaction. They use and develop new methods to integrate, visualize and query the large amounts of information available and in such a way come to new biological discoveries. A particular focus of the group is proteomics and post-translational modifications.








Yves Peeters, Laboratory of Biochemistry, Molecular and Structural Biology
After completing his master thesis at KU Leuven, Yves obtained an IWT fellowship for his PhD work in the field of synthetic biology. His primary interest goes to DNA polymerases and their modifications towards creation of artificial nucleic acids.





Details


DATE:
07.09.2015 10:00 – 19:00
VENUE:
KU Leuven Campus Arenberg, Celestijnenlaan 200A (Computer Science) aula 00.225, Heverlee, Belgium



PROGRAM


KU Leuven iGEM 2015 Symposium
on Synthetic Biology, Cell Systems and Ethics in Biochemistry
Leuven 07.09.2015
9:00-10:00 Registration and welcome tea/coffee
Morning Block 10:00-10:10 Welcome words by Prof. Johan Robben
10:10-10:35 Home speaker: Vera van Noort
Center for Microbial and Plant Genetics
"Lessons from systems biology of a minimal organism for synthetic biology"
10:35-11:35 Keynote speaker: Victor Dillard
Chief Operating Officer & Founder, Desktop Genetics
"Through synthetic biology to entrepreneurship"
Presentation by the iGEM Teams
11:40-11:50 iGEM Paris-Saclay: "SafetE.coli"
11:55-12:05 iGEM TU Eindhoven: "Click Coli"
12:05-13:05 Lunch Break, networking
Early Afternoon Block 13:05-14:05 Keynote speaker: Sebastian Maerkl
École Polytechnique Fédérale de Lausanne, LBNC
"Cell-Free synthetic biology"
14:05-14:30 Home speaker: Yves Peeters
Laboratory of Biochemistry: Molecular and Structural Biology
"Directed evolution of polymerases using synthetic biology methods"
Presentation by the iGEM Teams
14:35-14:45 iGEM Amsterdam: "[Photo]Synthetic Romance"
14:50-15:00 iGEM TU Darmstadt: "Building with light/Labsurfing"
15:05-15:20 iGEM KU Leuven: "Spot E.Shape"
15:25-15:50 Tea/Coffee break
Evening block 15:50-16:00 Introduction of the debate experts: Prof. Bart De Moor (KU Leuven), Prof. Johan Robben (KU Leuven), Dr. Stijn Bruers (UGent), Prof. Vera van Noort (KU Leuven), Victor Dillard (Desktop Genetics).
Moderator: Prof. Piet Van der Meer (Ugent/VUB)
16:00-17:00 A debate on the ethics in synthetic biology and biochemistry
17:00-17:10 Closing words
17:10-19:00 Wok and Talk Chinese dinner reception
19:30 Leuven Kermis – Visit to Leuven Centrum for interested people




Drinks and food

Beverages, lunch sandwiches and dinner-reception where be provided for all the participants free of charge.



Map




Survey

We conducted a survey to shed light on the perception of synthetic biology in Belgium.

Public Engagement

How we engaged generally and intra university-wise.

Ethics

What we learned from a panel of experts we invited to discuss ethical and legal questions.

Read more
Read more
Read more

Education

We went teaching and devised an educational card game, which is fun to play.

Collaborations

We worked together with other teams to improve both projects.

Back

Go back to the Outreach page.

Read more
Read more
Read more
Survey

We did a survey to shed light on the perception of synthetic biology in belgium.

Public Engagement

How we engaged generally and intra university-wise.

Ethics

We invited a panel of experts to discuss ethical questions with us.

Education

We went teaching and devised an educational card game, which is fun to play.

Collaborations

We worked together with other teams to improve both our projects.

Back

Go back to the Outreach page.

Contact

Address: Celestijnenlaan 200G room 00.08 - 3001 Heverlee
Telephone: +32(0)16 32 73 19
Email: igem@chem.kuleuven.be