Difference between revisions of "Team:Carnegie Mellon/Protocols"

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<div id = "flip1">Heyo</div>
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<div id = "flip_proteinextraction">His-Tag Soluble Protein Extraction</div>
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<div id = "panel1">wow, did this work</div>
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<div id = "panel_proteinextraction"><ol>
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  <li>Centrifuge 1.5 ml of culture for 1min full speed at 15000 rpm.</li>
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  <li>Remove supernatant.</li>
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  <li>Add 250 µl of extraction buffer (1% octyl-beta-thioglucoside in 10mM Tris-Cl, pH7.5).</li>
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  <li>Incubate at room temperature for 10 min.</li></ol>
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  <b>Wash Beads</b>
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  <ol><li>Remove 50 µl of Ni-NTA in 10 mM Trischloride bead solution and place in new tube for each purification.</li>
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  <li>Add 1 mL buffer (see table).</li>
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  <li>Centrifuge at 800 rpm for 30 seconds.</li>
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  <li>Remove supernatant.</li>
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  <li>Repeat 3 times and resuspend in 1 mL of Bead Wash Solution.</li>
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  </ol></div>
  
 
<div id = "estrogen_sensor">Estrogen Sensor</div>
 
<div id = "estrogen_sensor">Estrogen Sensor</div>

Revision as of 20:18, 9 July 2015

Under Construction.

This is almost as well-documented as Kim Kardashian's wedding.

Click to slide the panel down or up
Hello world!
His-Tag Soluble Protein Extraction
  1. Centrifuge 1.5 ml of culture for 1min full speed at 15000 rpm.
  2. Remove supernatant.
  3. Add 250 µl of extraction buffer (1% octyl-beta-thioglucoside in 10mM Tris-Cl, pH7.5).
  4. Incubate at room temperature for 10 min.
Wash Beads
  1. Remove 50 µl of Ni-NTA in 10 mM Trischloride bead solution and place in new tube for each purification.
  2. Add 1 mL buffer (see table).
  3. Centrifuge at 800 rpm for 30 seconds.
  4. Remove supernatant.
  5. Repeat 3 times and resuspend in 1 mL of Bead Wash Solution.
Estrogen Sensor
Estrogen Sensor Protocol