Difference between revisions of "Team:FAFU-CHINA/Description"

 
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<p>The Chinese Sacbrood Virus(CSBV) is one of the main factors contributing to the significant decrease of the amount of Chinese honeybee(Apis cerana cerana Fabricius) in the past 40 years. Since there is no an efficient therapeutic method so far, the preventative strategy is the only one the beekeepers can adopt currently.</p>
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<p>Chinese sacbrood virus (CSBV), one of the strains of the sacbrood virus (SBV), causes death of larvae of the Chinese honeybee (Apis cerana cerana) by forming a sac-like shape, resulting in failure of pupation. CSBV is one of the main causes of the significant decrease of Chinese honeybee populations in the past 40 years. So far, no effective control method exists for this bee disease, besides the prevention strategy, the only one beekeepers adopting currently.</p>
  
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<p>This year, with the RNA-interference technology, FAFU-China is aiming to cure this disease by silencing the CSBV’s gene, which is expressed as it’s RNA polymerase. To achieve this, two recombinant plasmids are necessary. In order to apply RNA-interference technology to production practice, our task is to clone RDRP gene into plasmid L4440, and have the plasmid transformed into the brewer yeast (saccharomyces cerevisiae). However, the brewer yeast is unable to identify the T7 promoter, which is exactly the promoter of RDRP gene fragment in the L4440, for it has no T7 RNA Polymerase, and because of this, another challenge for us is to recombine the plasmid PYES2 with the T7 RNA polymerase gene fragment, and similarly transform it into the brewer yeast. </p>
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<p>FAFU-China team aims to investigate the possibility of controlling this disease through silence of CSBV’s RdRp (RNA-dependent RNA polymerase) gene by using RNA-interference technology. Basicly, we try to clone the RdRp gene into plasmid L4440, and then transform the brewer yeast (Saccharomyces cerevisiae) with the modified plasmid. Due to lack of T7 RNA polymerase, the brewer yeast cannot identify the T7 promoter, which is exactly the promoter of RdRp gene fragment in L4440, Therefore, another challenge task for us is to recombine the plasmid PYES2 with the T7 RNA polymerase gene fragment, and then similarly transform the brewer yeast.</p>
  
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<p>Now, when the Chinese honeybee fed with the honey that contains the recombinant plasmid, the second stage of the treatment starts. With the plasmid PYES2, the yeast is able to replicate the double-stranded RNA. Neverthless, the dsRNA will soon be cut into pieces by the dicer, which is also known as endoribonuclease. After being cut by the dicer, the dsRNA become siRNA(Small interfering), and this is what causes the silence of the RDRP RNA information in the mRNA of the virus.</p>
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<p>When bees feed with honey containing the recombinant plasmid, the second stage of the treatment starts. With the plasmid PYES2, the yeast is able to replicate the double-stranded RNA (dsRNA), which nevertheless are soon cut into pieces by the dicer, also known as endoribonuclease. After being cut by the dicer, the dsRNA become siRNA (Small interfering RNA), which cause silence of RdRp gene.</p>
  
 
<h4>Inspiration</h4>
 
<h4>Inspiration</h4>

Latest revision as of 09:38, 15 July 2015




Indian Institute of Science Education and Research (IISER), Pune, India

Project Description

Tell us about your project, describe what moves you and why this is something important for your team.


What should this page contain?
  • A clear and concise description of your project.
  • A detailed explanation of why your team chose to work on this particular project.
  • References and sources to document your research.
  • Use illustrations and other visual resources to explain your project.

Advice on writing your Project Description

We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be consist, accurate and unambiguous in your achievements.

Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.


References

iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you though about your project and what works inspired you.


PROJECT DESCRIPTION

Chinese sacbrood virus (CSBV), one of the strains of the sacbrood virus (SBV), causes death of larvae of the Chinese honeybee (Apis cerana cerana) by forming a sac-like shape, resulting in failure of pupation. CSBV is one of the main causes of the significant decrease of Chinese honeybee populations in the past 40 years. So far, no effective control method exists for this bee disease, besides the prevention strategy, the only one beekeepers adopting currently.

FAFU-China team aims to investigate the possibility of controlling this disease through silence of CSBV’s RdRp (RNA-dependent RNA polymerase) gene by using RNA-interference technology. Basicly, we try to clone the RdRp gene into plasmid L4440, and then transform the brewer yeast (Saccharomyces cerevisiae) with the modified plasmid. Due to lack of T7 RNA polymerase, the brewer yeast cannot identify the T7 promoter, which is exactly the promoter of RdRp gene fragment in L4440, Therefore, another challenge task for us is to recombine the plasmid PYES2 with the T7 RNA polymerase gene fragment, and then similarly transform the brewer yeast.

When bees feed with honey containing the recombinant plasmid, the second stage of the treatment starts. With the plasmid PYES2, the yeast is able to replicate the double-stranded RNA (dsRNA), which nevertheless are soon cut into pieces by the dicer, also known as endoribonuclease. After being cut by the dicer, the dsRNA become siRNA (Small interfering RNA), which cause silence of RdRp gene.

Inspiration

See how other teams have described and presented their projects: