Difference between revisions of "Team:Stony Brook"

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<h2 id=sbu> Stony Brook iGEM 2015 </h2>
 
<h2 id=sbu> Stony Brook iGEM 2015 </h2>
 
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<br>
 
<h2 id = "sbu"> Project Description </h2>
 
<h2 id = "sbu"> Project Description </h2>
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<h4>Background</h4>
 
<h4>Background</h4>
 
<p> The human body has many feedback systems to maintain a suitable environment for its cells.  In its simplest form, a homeostatic system consists of a sensor and a response that the sensor can trigger.  Our project investigates recreating such a system using E. Coli to determine the robustness and reliability of such an endeavor.  Our project has its roots in type II diabetes, when we thought about how people with this disease cannot get rid of excess glucose in their blood naturally.  We imagined a system in which E. Coli detect high levels of glucose, and help the human body eliminate it.  Most importantly, we decided to try and do this without the use of insulin in any way, due to the insulin insensitivity of the afflicted.</p>
 
<p> The human body has many feedback systems to maintain a suitable environment for its cells.  In its simplest form, a homeostatic system consists of a sensor and a response that the sensor can trigger.  Our project investigates recreating such a system using E. Coli to determine the robustness and reliability of such an endeavor.  Our project has its roots in type II diabetes, when we thought about how people with this disease cannot get rid of excess glucose in their blood naturally.  We imagined a system in which E. Coli detect high levels of glucose, and help the human body eliminate it.  Most importantly, we decided to try and do this without the use of insulin in any way, due to the insulin insensitivity of the afflicted.</p>
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<h4>Future Plans</h4>
 
<h4>Future Plans</h4>
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<p> Our hopes for our system are to create cells which successfully create our tripeptide in response to glucose, and cells that successfully express our protease.  Then we hope to put them together to study their interactions.  Besides creating our system, we hope to investigate a better sensor, as well as perhaps how to design specific sensors.  EnvZ is a histidine kinase, which has a cytoplasmic domain and a periplasmic domain.  The periplasmic domain seems to be the part that controls what triggers the protein, while the cytoplasmic domain controls what happens when it triggers.  We want to research possible ways to replace the periplasmic domain while retaining the cytoplasmic domain to possibly create a sensor that can detect other things, like glucose specifically.</p>
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<p> Our hopes for our system are to create cells which successfully create our tripeptide in response to glucose, and cells that successfully express our protease.  Then we hope to put them together to study their interactions.  Besides creating our system, we hope to investigate a better sensor, as well as perhaps how to design specific sensors.  EnvZ is a histidine kinase, which has a cytoplasmic domain and a periplasmic domain.  The periplasmic domain seems to be the part that controls what triggers the protein, while the cytoplasmic domain controls what happens when it triggers.  We want to research possible ways to replace the periplasmic domain while retaining the cytoplasmic domain to possibly create a sensor that can detect other things, like glucose specifically.</p>-->
  
  
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Revision as of 18:02, 4 August 2015

Stony Brook iGEM 2015


Project Description