Difference between revisions of "Team:UMaryland/Composite Part"

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<!--Attention! If you are not part of the website team, you are NOT allowed to touch anything above this line without the express permission of Best Kohai.-->
 
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<style type="text/css">
  
<h2> Composite Parts</h2>
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#partcontainer {
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overflow:hidden;
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background-color:#66FF00;
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padding:5px 5px 5px 20px;
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#partname h2{
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font-size:30pt;
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color:#ffffff;
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#partdescription{
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width:400px;
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min-height:200px;
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background-color:#ffffff;
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padding:10px;
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float:left;
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margin:10px;
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border: 2px solid black;
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<h2>Miraculin Generator</h2>
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<div id='partdescription'>
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Combines BBa_K206000 (pBAD promoter) with BBa_K1033120 (B0034-ExpTag-Linker-Miraculin-Linker-6xHis). Codes for a His-tagged miraculin with export tag to periplasm. Induced with arabinose. Miraculin is a protein that interacts with our taste receptors, causing us to taste sour foods as sweet. This part was assembled using 3A Assembly as a training exercise for new UMaryland iGEM members.
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<img src="https://static.igem.org/mediawiki/2015/5/51/000.jpg" height="337" width="457">
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<h2>Hok/Sok coding sequence</h2>
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<div id='partdescription'>
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Codes for the Hok/Sok toxin system. This system naturally evolved in bacteria, possibly through a selfish gene paragiagm, and is used to keep plasmids.
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<div id='partimage'>
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<img src="https://static.igem.org/mediawiki/2015/a/a0/001.jpg" height="337" width="457">
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<h2>Unstable RFP</h2>
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Complete with a constitutive promotor, this fluorescent protein is expressed continuously throughout the cell. The half life of the protein is about 1 hour, as proteases have a high affinity for it.
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<img src="https://static.igem.org/mediawiki/2015/1/11/002.jpg" height="337" width="457">
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<h2>Hok/Sok and RFP</h2>
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<div id='partdescription'>
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Combination of parts K_001 and K_002. This part allows the quantitative testing of
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<img src="https://static.igem.org/mediawiki/2015/9/9c/003.jpg" height="337" width="457">
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<!--Attention! If you are not part of the website team, you are NOT allowed to touch anything above this line without the express permission of Best Kohai.-->
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<h2> Composite Parts</h2>
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<h2> Basic Parts</h2>
  
 
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.part-container .part-example .name{  
 
.part-container .part-example .name{  
 
    
 
    
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font-family: "Avant Garde", Avantgarde, "Century Gothic", CenturyGothic, "AppleGothic", sans-serif;
 
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.part-container .part-example .catalog{  
 
.part-container .part-example .catalog{  
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   font-size: 20px;  
 
   font-family: "Avant Garde", Avantgarde, "Century Gothic", CenturyGothic, "AppleGothic", sans-serif;
 
   font-family: "Avant Garde", Avantgarde, "Century Gothic", CenturyGothic, "AppleGothic", sans-serif;
 
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   background: rgba(0,0,0,0.9);
 
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   <div id = "Part 1">
 
   <div id = "Part 1">
 
   <div class = "part-example col-sm-6">
 
   <div class = "part-example col-sm-6">
     <div class = "name"> <b> Miraculin Generator </b> </div>  
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     <div class = "name"> <b> Basic 1 </b> </div>
       <div class = "catalog"> <a href = http://parts.igem.org/wiki/index.php/Part:BBa_K1783000 /a>  K1783000 </div>
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       <div class = "catalog"> Basic 1 Catalog no </div>
      <div class = desc>
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        <h3> Miraculin Gene with pBAD promoter
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        </h3>
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          <p>
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          pBAD-B0034-ExpTag-Linker-Miraculin-Linker-6xHis. Adds a promoter to BBa_K1033120 in order to characterize it. Under arabinose induction, produces miraculin connected to an export tag and a His-tag via unstructured linkers. Miraculin is a taste modifying protein.
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          </p>
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  <div id = "Part 2">
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  <div class = "part-example col-sm-6">
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    <div class = "name"> <b> Unstable RFP under Hok-Sok Regulation </b> </div>
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      <div class = "catalog"> <a href = http://parts.igem.org/wiki/index.php/Part:BBa_K1783003 /a>  K1783003 </div>
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       <div class = desc>
 
       <div class = desc>
         <h3> Hok-Sok gene linked to unstable RFP
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         <h3> Part 1 Gene
 
         </h3>
 
         </h3>
 
           <p>
 
           <p>
           Produces unstable RFP under a constitutive promoter. This construct carries the hok-sok toxin-antitoxin (TA) plasmid maintenance system and was used to measure the ability of this alternative plasmid maintenance system to keep plasmids within the cell without the use of antibiotics.
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           Part Notes
 
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Revision as of 18:56, 7 September 2015