Difference between revisions of "Team:Edinburgh/Basic Part"

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<div class="panel panel-default">
 
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  <div class="panel-heading">
 
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    <h3 class="panel-title">Panel title</h3>
 
 
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</div>
 
</div>
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    <div class="panel-group" id="accordion" role="tablist" aria-multiselectable="true">
 
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      <div class="panel panel-default">
 
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        <div class="panel-heading" role="tab" id="headingOne">
 
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          <h4 class="panel-title">
 
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            <a role="button" data-toggle="collapse" data-parent="#accordion" href="#collapseOne" aria-expanded="false" aria-controls="collapseOne">
 
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              1% Agarose Gel
 
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            </a>
 
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          </h4>
 
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        </div>
 
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        <div id="collapseOne" class="panel-collapse collapse in" role="tabpanel" aria-labelledby="headingOne">
 
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          <div class="panel-body">
 
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            <div class="col-md-6">
 
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              <p style="color: black;">
 
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              <h2>Materials</h2>
 
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              <ul>
 
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                <li>1g Agarose
 
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                <li>100ml 1X TAE buffer
 
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                <li>5µl GelRed stain
 
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              </ul>
 
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            </p>
 
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            </div>
 
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            <div class="col-md-6">
 
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              <p class="text-muted">
 
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              <h2>Procedure</h2>
 
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              <ul>
 
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                <li>1. Mix the agarose with the 1X TAE buffer in a flask.
 
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                <li>2. Heat the mixture until all the agarose is dissolved.
 
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                <li>3. Swirl the flask under cold running water to cool the mixture.
 
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                <li> 4. Add the gel stain.
 
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                <li>5. Pour into an assembled gel tray and let it cool.
 
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              </uL>
 
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            </p>
 
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          </div>
 
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            <a class="collapsed" role="button" data-toggle="collapse" data-parent="#accordion" href="#collapseTwo" aria-expanded="false" aria-controls="collapseTwo">
 
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            Agarose Gel Electrophoresis
 
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            <div class="col-md-6">
 
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              <p>
 
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              <h2>Materials</h2>
 
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              <ul>
 
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                <li>1% Agarose
 
−
                <li>1X TAE buffer
 
−
                <li>5X loading dye
 
−
                <li>DNA ladder
 
−
                <li>DNA samples
 
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              </ul>
 
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            </p>
 
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            </div>
 
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            <div class="col-md-6">
 
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              <p>
 
−
              <h2>Procedure</h2>
 
−
              <ul>
 
−
                <li>1. Place gel tray into the electrophoresis apparatus.
 
−
                <li>2. Pour 1X TAE so that the gel is covered by buffer.
 
−
                <li>3. Prepare the samples by adding the appropriate amount of loading dye.
 
−
                <li>4. Load samples and DNA ladder into wells on the gel.
 
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                <li>5. Run the gel at roughly 100V for around an hour
 
  
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              </uL>
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            </p>
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            </div>
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        <div class="panel-heading" role="tab" id="headingThree">
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          <h4 class="panel-title">
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            <a class="collapsed" role="button" data-toggle="collapse" data-parent="#accordion" href="#collapseThree" aria-expanded="false" aria-controls="collapseThree">
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              Culture
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            </a>
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          </h4>
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        <div id="collapseThree" class="panel-collapse collapse" role="tabpanel" aria-labelledby="headingThree">
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          <div class="panel-body">
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          <div class="col-md-6">
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              <p>
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              <h2>Materials</h2>
+
−
              <ul>
+
−
                <li>10ml Luria Broth (LB)
+
−
                <li>10µl Specific Antibiotic at 1000x (Chloramphenicol, Ampicillin or Kanamycin)
+
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                <li>Loop (for picking colony)
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−
                <li>Ethanol
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              </ul>
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            </p>
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            </div>
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            <div class="col-md-6">
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              <p>
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              <h2>Procedure</h2>
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              <ul>
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−
                <li>1. Pour 10ml of LB into a 50ml Falcon tube.
+
−
                <li>2. Pipette 10µl of antibiotic into the broth.
+
−
                <li>3. Dip loop in ethanol and flame to sterilise. Once it is cool, pick colony and transfer to a 50ml Falcon tube.
+
−
                <li>4. Incubate at 37°C overnight in a shaking incubator.
+
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              </uL>
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            </p>
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            </div>
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      <div class="panel panel-default">
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        <div class="panel-heading" role="tab" id="headingFour">
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          <h4 class="panel-title">
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            <a class="collapsed" role="button" data-toggle="collapse" data-parent="#accordion" href="#collapseFour" aria-expanded="false" aria-controls="collapseFour">
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            Gel Purification using QIAquick Gel Extraction Kit
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            </a>
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          </h4>
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        </div>
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      </div>
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    </div>
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  </div>
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       <footer>
 
       <footer>

Revision as of 10:33, 1 September 2015

Basic Parts




Panel title

Materials

  • 1g Agarose
  • 100ml 1X TAE buffer
  • 5µl GelRed stain

Procedure

  • 1. Mix the agarose with the 1X TAE buffer in a flask.
  • 2. Heat the mixture until all the agarose is dissolved.
  • 3. Swirl the flask under cold running water to cool the mixture.
  • 4. Add the gel stain.
  • 5. Pour into an assembled gel tray and let it cool.

Materials

  • 1% Agarose
  • 1X TAE buffer
  • 5X loading dye
  • DNA ladder
  • DNA samples

Procedure

  • 1. Place gel tray into the electrophoresis apparatus.
  • 2. Pour 1X TAE so that the gel is covered by buffer.
  • 3. Prepare the samples by adding the appropriate amount of loading dye.
  • 4. Load samples and DNA ladder into wells on the gel.
  • 5. Run the gel at roughly 100V for around an hour

Materials

  • 10ml Luria Broth (LB)
  • 10µl Specific Antibiotic at 1000x (Chloramphenicol, Ampicillin or Kanamycin)
  • Loop (for picking colony)
  • Ethanol

Procedure

  • 1. Pour 10ml of LB into a 50ml Falcon tube.
  • 2. Pipette 10µl of antibiotic into the broth.
  • 3. Dip loop in ethanol and flame to sterilise. Once it is cool, pick colony and transfer to a 50ml Falcon tube.
  • 4. Incubate at 37°C overnight in a shaking incubator.

Panel title

Materials

  • 1g Agarose
  • 100ml 1X TAE buffer
  • 5µl GelRed stain

Procedure

  • 1. Mix the agarose with the 1X TAE buffer in a flask.
  • 2. Heat the mixture until all the agarose is dissolved.
  • 3. Swirl the flask under cold running water to cool the mixture.
  • 4. Add the gel stain.
  • 5. Pour into an assembled gel tray and let it cool.

Materials

  • 1% Agarose
  • 1X TAE buffer
  • 5X loading dye
  • DNA ladder
  • DNA samples

Procedure

  • 1. Place gel tray into the electrophoresis apparatus.
  • 2. Pour 1X TAE so that the gel is covered by buffer.
  • 3. Prepare the samples by adding the appropriate amount of loading dye.
  • 4. Load samples and DNA ladder into wells on the gel.
  • 5. Run the gel at roughly 100V for around an hour

Materials

  • 10ml Luria Broth (LB)
  • 10µl Specific Antibiotic at 1000x (Chloramphenicol, Ampicillin or Kanamycin)
  • Loop (for picking colony)
  • Ethanol

Procedure

  • 1. Pour 10ml of LB into a 50ml Falcon tube.
  • 2. Pipette 10µl of antibiotic into the broth.
  • 3. Dip loop in ethanol and flame to sterilise. Once it is cool, pick colony and transfer to a 50ml Falcon tube.
  • 4. Incubate at 37°C overnight in a shaking incubator.

Panel title

Materials

  • 1g Agarose
  • 100ml 1X TAE buffer
  • 5µl GelRed stain

Procedure

  • 1. Mix the agarose with the 1X TAE buffer in a flask.
  • 2. Heat the mixture until all the agarose is dissolved.
  • 3. Swirl the flask under cold running water to cool the mixture.
  • 4. Add the gel stain.
  • 5. Pour into an assembled gel tray and let it cool.

Materials

  • 1% Agarose
  • 1X TAE buffer
  • 5X loading dye
  • DNA ladder
  • DNA samples

Procedure

  • 1. Place gel tray into the electrophoresis apparatus.
  • 2. Pour 1X TAE so that the gel is covered by buffer.
  • 3. Prepare the samples by adding the appropriate amount of loading dye.
  • 4. Load samples and DNA ladder into wells on the gel.
  • 5. Run the gel at roughly 100V for around an hour

Materials

  • 10ml Luria Broth (LB)
  • 10µl Specific Antibiotic at 1000x (Chloramphenicol, Ampicillin or Kanamycin)
  • Loop (for picking colony)
  • Ethanol

Procedure

  • 1. Pour 10ml of LB into a 50ml Falcon tube.
  • 2. Pipette 10µl of antibiotic into the broth.
  • 3. Dip loop in ethanol and flame to sterilise. Once it is cool, pick colony and transfer to a 50ml Falcon tube.
  • 4. Incubate at 37°C overnight in a shaking incubator.