Difference between revisions of "Team:EPF Lausanne/Part Collection"
E.Cuillery (Talk | contribs) |
E.Cuillery (Talk | contribs) |
||
| Line 18: | Line 18: | ||
<div class="row"> | <div class="row"> | ||
| + | <br><br> | ||
<div class="col-md-3 text-center"> | <div class="col-md-3 text-center"> | ||
<img src="https://static.igem.org/mediawiki/2015/2/25/EPF_Lausanne_Parts_dCas9-w.png" style="width:60%"> | <img src="https://static.igem.org/mediawiki/2015/2/25/EPF_Lausanne_Parts_dCas9-w.png" style="width:60%"> | ||
Revision as of 11:54, 7 September 2015
dCas9-ω
dCa9s-ω is our main tool to build our project in E. coli. This catalytically dead Cas9 fused to the RNA polymerase ω subunit can act as an activator or as an inhibitor when provided the right guide RNA.
PAM rich URS J23117
This promoter is composed of the weak J23117 promoter, BBa_J23117, fused to a PAM rich upstream regulatory region (URS) that is needed for dCas9 to bind as dCas9 cannot bind without a PAM sequence.
