Since our project involved expression of many antigenic peptides we decided to share those antigen-sequences with the iGEM community. We obtained most of the sequences via paper research and we would like to give special thanks to the group of Prof. Dr. Michael Hust (TU Braunschweig) who provided us with expression plasmids for the Salmonella Typhimurium antigen and a corresponding single chain variable fragment. The codon optimization tool from Integrated DNA Technologies was used to improve most sequences for expression in E. coli. We also removed all restriction sites that are not allowed in RFC[10], so that all sequences are compatible with the submission vector pSB1C3. When we started working with our first plasmids for this project, we decided to use a specific nomenclature. Every plasmid name starts with “pIG15” which is short for “plasmid igem 2015”. According to this, we named the plasmids containing our biobricks in the shipping backbone pRIG15 (“pRIG” as in “brick”)

In the table below we listed all our biobricks and our biobrick improvement, pOP. We decided on pOP as our favorite biobrick because it provides the iGEM communitiy with an igem conform backbone for protein expression. When we started our project we faced the problem of pSB1C3 being a plasmid designated for cloning colliding with our need of a plasmid for expression of proteins. To be able to express proteins in a vector that exhibits all the properties of an iGEM standard backbone, we improved pSB1C3 (or whatever biobrick we improved) in a way that makes it possible to express proteins in E.coli. Details on which parts of pSB1C3 we changed and how we did this can be found here.