Difference between revisions of "Team:Austin UTexas/Project/Plasmid Study"

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<p>Plasmids were constructed with standard BioBrick cloning procedures and enzymes. Composite plasmids were constructed using the following BioBrick combinations:</p>
 
<p>Plasmids were constructed with standard BioBrick cloning procedures and enzymes. Composite plasmids were constructed using the following BioBrick combinations:</p>
 
<div align=center>
 
<div align=center>
−
<font color="017e70"><b>REPLACE LIST WITH TABLE AND/OR FIGURE</b></font>
+
 
−
BBa_K864100+BBa_K314100<p>
+
 
−
BBa_E0020+BBa_K608007</p><p>
+
<table style="width:60%">
−
BBa_EE030+BBa_K608006</p><p>
+
 
−
BBa_E0020+BBa_K608003</p><p>
+
  <tr>
−
BBa_E0030+BBa_K608007</p><p>
+
    <td><b><u>Insert BioBrick</u></b></td>
−
BBa_K592101+BBa_K608006</p><p>
+
    <td><b><u>Insert Phenotype</u></b></td>
−
BBa_K592100+BBa_K608004</p><p>
+
    <td><b><u>Vector BioBrick</u></b></td>
−
BBa_K592101+BBa_K608002</p><p>
+
  </tr>
−
BBa_K864100+BBa_K608006</p><p>
+
<tr></tr>
−
BBa_E0030+BBa_K314100</p><p>
+
<tr>
−
BBa_E0020+BBa_K608002</p><p>
+
    <td>BBa_K592100</td>
−
BBa_K864100+BBa_K608002</p><p>
+
    <td>BFP</td>
−
BBa_E0030+BBa_K314100</p><p>
+
    <td>BBa_K608004</td>
−
BBa_K592101+BBa_K608007</p><p>
+
  </tr>
 +
  <tr>
 +
    <td>BBa_E0020</td>
 +
    <td>ECFP</td>
 +
    <td>BBa_K608007</td>
 +
  </tr>
 +
<tr>
 +
    <td>BBa_E0020</td>
 +
    <td>ECFP</td>
 +
    <td>BBa_K608003</td>
 +
  </tr>
 +
<tr>
 +
    <td>BBa_E0020</td>
 +
    <td>ECFP</td>
 +
    <td>BBa_K608002</td>
 +
  </tr>
 +
<tr>
 +
    <td>BBa_K592101</td>
 +
    <td>YFP</td>
 +
    <td>BBa_K608002</td>
 +
  </tr>
 +
<tr>
 +
    <td>BBa_K592101</td>
 +
    <td>YFP</td>
 +
    <td>BBa_K608006</td>
 +
  </tr>
 +
<tr>
 +
    <td>BBa_K592101</td>
 +
    <td>YFP</td>
 +
    <td>BBa_K608007</td>
 +
  </tr>
 +
<tr>
 +
    <td>BBa_K864100</td>
 +
    <td>SYFP</td>
 +
    <td>BBa_K608002</td>
 +
  </tr>
 +
<tr>
 +
    <td>BBa_K864100</td>
 +
    <td>SYFP</td>
 +
    <td>BBa_K314100</td>
 +
  </tr>
 +
<tr>
 +
    <td>BBa_864100</td>
 +
    <td>SYFP</td>
 +
    <td>BBa_K608006</td>
 +
  </tr>
 +
<tr>
 +
    <td>BBa_E0030</td>
 +
    <td>EYFP</td>
 +
    <td>BBa_K608007</td>
 +
  </tr>
 +
<tr>
 +
    <td>BBa_EE030</td>
 +
    <td>EYFP</td>
 +
    <td>BBa_K608006</td>
 +
  </tr>
 +
<tr>
 +
    <td>BBa_E0030</td>
 +
    <td>EYFP</td>
 +
    <td>BBa_K314100</td>
 +
  </tr>
 +
<tr>
 +
    <td>BBa_E0030</td>
 +
    <td>EYFP</td>
 +
    <td>BBa_K314100</td>
 +
  </tr>
 +
 
 +
 
 +
 
 +
 
 +
</table>
 +
 
 +
 
 
<div align=left>
 
<div align=left>
 
<br>
 
<br>

Revision as of 21:33, 13 September 2015

--this is a first draft--

Spring 2015 Data Set

Plasmid Construction and Transformation


Plasmids were constructed with standard BioBrick cloning procedures and enzymes. Composite plasmids were constructed using the following BioBrick combinations:


Insert BioBrick Insert Phenotype Vector BioBrick
BBa_K592100 BFP BBa_K608004
BBa_E0020 ECFP BBa_K608007
BBa_E0020 ECFP BBa_K608003
BBa_E0020 ECFP BBa_K608002
BBa_K592101 YFP BBa_K608002
BBa_K592101 YFP BBa_K608006
BBa_K592101 YFP BBa_K608007
BBa_K864100 SYFP BBa_K608002
BBa_K864100 SYFP BBa_K314100
BBa_864100 SYFP BBa_K608006
BBa_E0030 EYFP BBa_K608007
BBa_EE030 EYFP BBa_K608006
BBa_E0030 EYFP BBa_K314100
BBa_E0030 EYFP BBa_K314100



discuss what these BioBricks are
plasmids transformed by electroporation into pSB1c3



Fluorescence Readings

Fig.2 ^^^REPLACE WITH BETTER RESOLUTION

relative fluorescence units
96 well plate
fluorescence data summary
summary figure(?)

Protocol